Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: Seroprevalence and genotypes
Data on the genotypes of Toxoplasma gondii circulating in wildlife are scarce. In the present study, foxes and rodents from two Federal States in Central or Eastern Germany were examined for T. gondii infections. Body fluids were collected at necropsy or fluids were obtained from frozen tissues of n...
Published in: | Veterinary Parasitology |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.vetpar.2011.10.030 https://www.openagrar.de/receive/fimport_mods_00001227 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00001533/SD201288.pdf http://www.sciencedirect.com/science/article/pii/S0304401711006893 |
id |
ftopenagrar:oai:www.openagrar.de:fimport_mods_00001227 |
---|---|
record_format |
openpolar |
spelling |
ftopenagrar:oai:www.openagrar.de:fimport_mods_00001227 2024-09-15T18:18:47+00:00 Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: Seroprevalence and genotypes Herrmann, Daland Maksimov, Pavlo Maksimov, Aline Sutor, Astrid Siglinde Schwarz, Sabine Jaschke, W. Schliephake, A. Denzin, Nicolai Conraths, Franz Josef Schares, Gereon 2011-10-29 https://doi.org/10.1016/j.vetpar.2011.10.030 https://www.openagrar.de/receive/fimport_mods_00001227 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00001533/SD201288.pdf http://www.sciencedirect.com/science/article/pii/S0304401711006893 eng eng Veterinary parasitology -- Vet. Parasitol. -- 0304-4017 -- 1873-2550 -- 196831-2 -- 1498947-5 -- https://www.sciencedirect.com/journal/veterinary-parasitology-regional-studies-and-reports/issues -- http://www.bibliothek.uni-regensburg.de/ezeit/?2851859 https://doi.org/10.1016/j.vetpar.2011.10.030 https://www.openagrar.de/receive/fimport_mods_00001227 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00001533/SD201288.pdf http://www.sciencedirect.com/science/article/pii/S0304401711006893 all rights reserved only signed in user info:eu-repo/semantics/restrictedAccess article Text ddc:570 Genotyping RFLP Foxes WILDLIFE article Text doc-type:article 2011 ftopenagrar https://doi.org/10.1016/j.vetpar.2011.10.030 2024-08-12T23:39:04Z Data on the genotypes of Toxoplasma gondii circulating in wildlife are scarce. In the present study, foxes and rodents from two Federal States in Central or Eastern Germany were examined for T. gondii infections. Body fluids were collected at necropsy or fluids were obtained from frozen tissues of naturally exposed red foxes (Vulpes vulpes), voles (Microtus arvalis), shrews (Neomys anomalus) and a striped field mouse (Apodemus agrarius) and tested for T. gondii by serology. DNA isolated from tissues of seropositive foxes and all the rodents was examined by PCR. In the German Federal States of Brandenburg and Saxony-Anhalt 152/204 (74.5%) and 149/176 (84.7%) of foxes, respectively, but none of the rodents (0/72) had antibodies to T. gondii. Only 28/152 (18.4%) and 20/149 (13.4%) of seropositive foxes from Brandenburg and Saxony-Anhalt, respectively, but none of the rodents tested PCR-positive for T. gondii. The complete T. gondii genotype could be determined for twelve samples using nine PCR-restriction fragment length polymorphism (PCR-RFLP) markers (newSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, L358 and Apico). In addition to T. gondii clonal type II (Apico II) and type II (Apico I), type III and T. gondii genotypes showing non-canonical allele patterns were observed in foxes. This suggests that, while T. gondii type II prevails in foxes, other genotypes circulate in wildlife. The population structure of T. gondii in Germany may be more diverse than previously thought. Article in Journal/Newspaper Microtus arvalis OpenAgrar (OA) Veterinary Parasitology 185 2-4 78 85 |
institution |
Open Polar |
collection |
OpenAgrar (OA) |
op_collection_id |
ftopenagrar |
language |
English |
topic |
article Text ddc:570 Genotyping RFLP Foxes WILDLIFE |
spellingShingle |
article Text ddc:570 Genotyping RFLP Foxes WILDLIFE Herrmann, Daland Maksimov, Pavlo Maksimov, Aline Sutor, Astrid Siglinde Schwarz, Sabine Jaschke, W. Schliephake, A. Denzin, Nicolai Conraths, Franz Josef Schares, Gereon Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: Seroprevalence and genotypes |
topic_facet |
article Text ddc:570 Genotyping RFLP Foxes WILDLIFE |
description |
Data on the genotypes of Toxoplasma gondii circulating in wildlife are scarce. In the present study, foxes and rodents from two Federal States in Central or Eastern Germany were examined for T. gondii infections. Body fluids were collected at necropsy or fluids were obtained from frozen tissues of naturally exposed red foxes (Vulpes vulpes), voles (Microtus arvalis), shrews (Neomys anomalus) and a striped field mouse (Apodemus agrarius) and tested for T. gondii by serology. DNA isolated from tissues of seropositive foxes and all the rodents was examined by PCR. In the German Federal States of Brandenburg and Saxony-Anhalt 152/204 (74.5%) and 149/176 (84.7%) of foxes, respectively, but none of the rodents (0/72) had antibodies to T. gondii. Only 28/152 (18.4%) and 20/149 (13.4%) of seropositive foxes from Brandenburg and Saxony-Anhalt, respectively, but none of the rodents tested PCR-positive for T. gondii. The complete T. gondii genotype could be determined for twelve samples using nine PCR-restriction fragment length polymorphism (PCR-RFLP) markers (newSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, L358 and Apico). In addition to T. gondii clonal type II (Apico II) and type II (Apico I), type III and T. gondii genotypes showing non-canonical allele patterns were observed in foxes. This suggests that, while T. gondii type II prevails in foxes, other genotypes circulate in wildlife. The population structure of T. gondii in Germany may be more diverse than previously thought. |
format |
Article in Journal/Newspaper |
author |
Herrmann, Daland Maksimov, Pavlo Maksimov, Aline Sutor, Astrid Siglinde Schwarz, Sabine Jaschke, W. Schliephake, A. Denzin, Nicolai Conraths, Franz Josef Schares, Gereon |
author_facet |
Herrmann, Daland Maksimov, Pavlo Maksimov, Aline Sutor, Astrid Siglinde Schwarz, Sabine Jaschke, W. Schliephake, A. Denzin, Nicolai Conraths, Franz Josef Schares, Gereon |
author_sort |
Herrmann, Daland |
title |
Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: Seroprevalence and genotypes |
title_short |
Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: Seroprevalence and genotypes |
title_full |
Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: Seroprevalence and genotypes |
title_fullStr |
Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: Seroprevalence and genotypes |
title_full_unstemmed |
Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: Seroprevalence and genotypes |
title_sort |
toxoplasma gondii in foxes and rodents from the german federal states of brandenburg and saxony-anhalt: seroprevalence and genotypes |
publishDate |
2011 |
url |
https://doi.org/10.1016/j.vetpar.2011.10.030 https://www.openagrar.de/receive/fimport_mods_00001227 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00001533/SD201288.pdf http://www.sciencedirect.com/science/article/pii/S0304401711006893 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_relation |
Veterinary parasitology -- Vet. Parasitol. -- 0304-4017 -- 1873-2550 -- 196831-2 -- 1498947-5 -- https://www.sciencedirect.com/journal/veterinary-parasitology-regional-studies-and-reports/issues -- http://www.bibliothek.uni-regensburg.de/ezeit/?2851859 https://doi.org/10.1016/j.vetpar.2011.10.030 https://www.openagrar.de/receive/fimport_mods_00001227 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00001533/SD201288.pdf http://www.sciencedirect.com/science/article/pii/S0304401711006893 |
op_rights |
all rights reserved only signed in user info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.vetpar.2011.10.030 |
container_title |
Veterinary Parasitology |
container_volume |
185 |
container_issue |
2-4 |
container_start_page |
78 |
op_container_end_page |
85 |
_version_ |
1810456857113788416 |