Novel organ-specific effects of Ketoprofen and its enantiomer, dexketoprofen on toxicological response transcripts and their functional products in salmon.
Racemic ketoprofen (RS-KP) and its enantiomer, dexketoprofen (S(+)-KP) are widely used non-steroidal anti-inflammatory drugs (NSAIDs), and commonly detected in the aquatic environment. The present study has evaluated the toxicological effects of RS-KP and S(+)-KP on biotransformation and oxidative s...
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ftopenaccessrep:oai:zenodo.org:82903 2023-10-25T01:36:44+02:00 Novel organ-specific effects of Ketoprofen and its enantiomer, dexketoprofen on toxicological response transcripts and their functional products in salmon. Augustine Arukwe Francesca Cappelli Luigi Intorre Valentina Meucci Elvira Mennillo Carlo Pretti Giacomo Luci 2020-08-21 https://www.openaccessrepository.it/record/82903 https://doi.org/10.1016/j.aquatox.2020.105677 eng eng url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/82903 doi:10.1016/j.aquatox.2020.105677 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ European Marine Science Health Toxicology and Mutagenesis Aquatic Science info:eu-repo/semantics/article publication-article 2020 ftopenaccessrep https://doi.org/10.1016/j.aquatox.2020.105677 2023-09-26T22:20:08Z Racemic ketoprofen (RS-KP) and its enantiomer, dexketoprofen (S(+)-KP) are widely used non-steroidal anti-inflammatory drugs (NSAIDs), and commonly detected in the aquatic environment. The present study has evaluated the toxicological effects of RS-KP and S(+)-KP on biotransformation and oxidative stress responses in gills and liver of Atlantic salmon. Fish were exposed for 10 days using different concentrations of RS-KP (1, 10 and 100 μg/L) and S(+)-KP (0.5, 5 and 50 μg/L). Biotransformation and oxidative stress responses were analysed at both transcript and functional levels. In the gills, significant inhibitory effect at transcriptional and enzymatic levels were observed for biotransformation and oxidative stress responses. On the contrary, biotransformation responses were significantly increased at transcriptional and translational levels in the liver, while the associated enzymatic activities did not parallel this trend and were inhibited and further demonstrated by principal component analysis (PCA). Our findings showed that both compounds produced comparable toxicological effects, by producing organ-specific effect differences. RS-KP and S(+)-KP did not bioaccumulate in fish muscle, either due to rapid metabolism or excretion as a result of their hydrophobic properties. Interestingly, the inhibitory effects observed in the gills suggest that these drugs may not undergo first pass metabolism, that might result to downstream differences in toxicological outcomes. Article in Journal/Newspaper Atlantic salmon Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository Aquatic Toxicology 229 105677 |
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Open Polar |
collection |
Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository |
op_collection_id |
ftopenaccessrep |
language |
English |
topic |
European Marine Science Health Toxicology and Mutagenesis Aquatic Science |
spellingShingle |
European Marine Science Health Toxicology and Mutagenesis Aquatic Science Augustine Arukwe Francesca Cappelli Luigi Intorre Valentina Meucci Elvira Mennillo Carlo Pretti Giacomo Luci Novel organ-specific effects of Ketoprofen and its enantiomer, dexketoprofen on toxicological response transcripts and their functional products in salmon. |
topic_facet |
European Marine Science Health Toxicology and Mutagenesis Aquatic Science |
description |
Racemic ketoprofen (RS-KP) and its enantiomer, dexketoprofen (S(+)-KP) are widely used non-steroidal anti-inflammatory drugs (NSAIDs), and commonly detected in the aquatic environment. The present study has evaluated the toxicological effects of RS-KP and S(+)-KP on biotransformation and oxidative stress responses in gills and liver of Atlantic salmon. Fish were exposed for 10 days using different concentrations of RS-KP (1, 10 and 100 μg/L) and S(+)-KP (0.5, 5 and 50 μg/L). Biotransformation and oxidative stress responses were analysed at both transcript and functional levels. In the gills, significant inhibitory effect at transcriptional and enzymatic levels were observed for biotransformation and oxidative stress responses. On the contrary, biotransformation responses were significantly increased at transcriptional and translational levels in the liver, while the associated enzymatic activities did not parallel this trend and were inhibited and further demonstrated by principal component analysis (PCA). Our findings showed that both compounds produced comparable toxicological effects, by producing organ-specific effect differences. RS-KP and S(+)-KP did not bioaccumulate in fish muscle, either due to rapid metabolism or excretion as a result of their hydrophobic properties. Interestingly, the inhibitory effects observed in the gills suggest that these drugs may not undergo first pass metabolism, that might result to downstream differences in toxicological outcomes. |
format |
Article in Journal/Newspaper |
author |
Augustine Arukwe Francesca Cappelli Luigi Intorre Valentina Meucci Elvira Mennillo Carlo Pretti Giacomo Luci |
author_facet |
Augustine Arukwe Francesca Cappelli Luigi Intorre Valentina Meucci Elvira Mennillo Carlo Pretti Giacomo Luci |
author_sort |
Augustine Arukwe |
title |
Novel organ-specific effects of Ketoprofen and its enantiomer, dexketoprofen on toxicological response transcripts and their functional products in salmon. |
title_short |
Novel organ-specific effects of Ketoprofen and its enantiomer, dexketoprofen on toxicological response transcripts and their functional products in salmon. |
title_full |
Novel organ-specific effects of Ketoprofen and its enantiomer, dexketoprofen on toxicological response transcripts and their functional products in salmon. |
title_fullStr |
Novel organ-specific effects of Ketoprofen and its enantiomer, dexketoprofen on toxicological response transcripts and their functional products in salmon. |
title_full_unstemmed |
Novel organ-specific effects of Ketoprofen and its enantiomer, dexketoprofen on toxicological response transcripts and their functional products in salmon. |
title_sort |
novel organ-specific effects of ketoprofen and its enantiomer, dexketoprofen on toxicological response transcripts and their functional products in salmon. |
publishDate |
2020 |
url |
https://www.openaccessrepository.it/record/82903 https://doi.org/10.1016/j.aquatox.2020.105677 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/82903 doi:10.1016/j.aquatox.2020.105677 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1016/j.aquatox.2020.105677 |
container_title |
Aquatic Toxicology |
container_volume |
229 |
container_start_page |
105677 |
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1780731728778756096 |