Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes
A SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s...
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ftopenaccessrep:oai:zenodo.org:63874 2023-05-15T15:27:05+02:00 Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes Maretto, F. Reffo, E. Dalvit, C. GIANNI BARCACCIA Mantovani, R. 2010-04-01 https://www.openaccessrepository.it/record/63874 https://doi.org/10.4081/ijas.2007.1s.161 eng eng url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/63874 doi:10.4081/ijas.2007.1s.161 info:eu-repo/semantics/openAccess Animal Science and Zoology info:eu-repo/semantics/article publication-article 2010 ftopenaccessrep https://doi.org/10.4081/ijas.2007.1s.161 2022-11-23T06:39:25Z A SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s rRNA gene was amplified using interspecific conserved primer and sequenced. The sequences were aligned and analyzed for the presence of SNPs; three SNPs (MerSNP1, MerSNP7 and MerSNP9) were identified and selected to allow discrimination between the four species. Aplotypes were TCC, CCC, CAT and CAC for Pacific cod, Atlantic cod, Haddock and Ling respectively. Confirmation of results was achieved by sequencing 16s rRNA gene fragments of 16 G. morhua, 7 G. macrocephalus, 15 M. aeglefinus and 5 M. molva samples collected at different fish catching campaign. Nucleotide sequence of 16s rRNA mitochondial gene has been shown to be a useful tool to allow rapid reliable and fully automatable for discrimination of 4 economically important species in fisheries industry. Article in Journal/Newspaper atlantic cod Gadus morhua Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository Pacific Italian Journal of Animal Science 6 sup1 161 163 |
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Open Polar |
collection |
Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository |
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ftopenaccessrep |
language |
English |
topic |
Animal Science and Zoology |
spellingShingle |
Animal Science and Zoology Maretto, F. Reffo, E. Dalvit, C. GIANNI BARCACCIA Mantovani, R. Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
topic_facet |
Animal Science and Zoology |
description |
A SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s rRNA gene was amplified using interspecific conserved primer and sequenced. The sequences were aligned and analyzed for the presence of SNPs; three SNPs (MerSNP1, MerSNP7 and MerSNP9) were identified and selected to allow discrimination between the four species. Aplotypes were TCC, CCC, CAT and CAC for Pacific cod, Atlantic cod, Haddock and Ling respectively. Confirmation of results was achieved by sequencing 16s rRNA gene fragments of 16 G. morhua, 7 G. macrocephalus, 15 M. aeglefinus and 5 M. molva samples collected at different fish catching campaign. Nucleotide sequence of 16s rRNA mitochondial gene has been shown to be a useful tool to allow rapid reliable and fully automatable for discrimination of 4 economically important species in fisheries industry. |
format |
Article in Journal/Newspaper |
author |
Maretto, F. Reffo, E. Dalvit, C. GIANNI BARCACCIA Mantovani, R. |
author_facet |
Maretto, F. Reffo, E. Dalvit, C. GIANNI BARCACCIA Mantovani, R. |
author_sort |
Maretto, F. |
title |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_short |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_full |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_fullStr |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_full_unstemmed |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_sort |
finding 16s rrna gene-based snps for the genetic traceability of commercial species belonging to gadiformes |
publishDate |
2010 |
url |
https://www.openaccessrepository.it/record/63874 https://doi.org/10.4081/ijas.2007.1s.161 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/63874 doi:10.4081/ijas.2007.1s.161 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.4081/ijas.2007.1s.161 |
container_title |
Italian Journal of Animal Science |
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6 |
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sup1 |
container_start_page |
161 |
op_container_end_page |
163 |
_version_ |
1766357551361818624 |