Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes
Abstract With the fast growth of today's aquaculture industry, the demand for aquafeeds is expanding dramatically. Insects, which are part of the natural diet of salmonids, could represent a sustainable ingredient for aquaculture feed. The aim of the current study was to test how a partial or t...
Published in: | Fish & Shellfish Immunology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2019
|
Subjects: | |
Online Access: | https://www.openaccessrepository.it/record/35898 https://doi.org/10.1016/j.fsi.2019.05.042 |
id |
ftopenaccessrep:oai:zenodo.org:35898 |
---|---|
record_format |
openpolar |
spelling |
ftopenaccessrep:oai:zenodo.org:35898 2023-05-15T15:31:28+02:00 Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes Oda Kvalsvik Stenberg Elisabeth Holen Luisa Piemontese Nina S. Liland Erik-Jan Lock Marit Espe Ikram Belghit 2019-08-01 https://www.openaccessrepository.it/record/35898 https://doi.org/10.1016/j.fsi.2019.05.042 eng eng url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/35898 doi:10.1016/j.fsi.2019.05.042 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ CC-BY-NC-ND info:eu-repo/semantics/article publication-article 2019 ftopenaccessrep https://doi.org/10.1016/j.fsi.2019.05.042 2022-11-23T06:46:21Z Abstract With the fast growth of today's aquaculture industry, the demand for aquafeeds is expanding dramatically. Insects, which are part of the natural diet of salmonids, could represent a sustainable ingredient for aquaculture feed. The aim of the current study was to test how a partial or total replacement of dietary fishmeal with insect meal affect gene responses involved in inflammation, the eicosanoid pathway and stress response in Atlantic salmon (Salmo salar L.) in isolated head kidney leukocytes after exposure to bacterial or viral mimic. Insect meal (IM) was produced from black soldier fly (BSF, Hermetia illucens) larvae. Seawater Atlantic salmon were fed three different diets for 8 weeks; a control diet (IM0, protein from fishmeal and plant based ingredients (25:75) and lipid from fish oil and vegetable oil (33:66); and two insect-meal containing diets, IM66 and IM100, where 66 and 100% of the fishmeal protein was replaced with IM, respectively. Leukocytes were isolated from the head kidney of fish (n = 6) from each of the three dietary groups. Isolated leukocytes were seeded into culture wells and added either a bacterial mimic (lipopolysaccharide, LPS) or a viral mimic (polyinosinic acid: polycytidylic acid, poly I: C) to induce an inflammatory response. Controls (Ctl) without LPS and poly I: C were included. The transcription of interleukins IL-1β, IL-8, IL-10 and TNF-α were elevated in LPS treated leukocytes isolated from salmon fed the three dietary groups (IM0, IM66 and IM100). The inflammatory-related gene expression in head kidney cells were, however, not affected by the pre-fed substitution of fish meal with IM in the diet of salmon. Gene transcriptions of PTGDS and PTGES were neither affected by LPS, poly I: C or the experimental diets fed prior to cell isolation, while salmon fed with IM showed a lower expression of LOX5. The gene expression of TLR22 and C/EBP-β were down-regulated by the LPS treatment in the cells isolated from salmon fed insect-based diets (IM66 and IM100) compared to ... Article in Journal/Newspaper Atlantic salmon Salmo salar Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository Fish & Shellfish Immunology 91 223 232 |
institution |
Open Polar |
collection |
Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository |
op_collection_id |
ftopenaccessrep |
language |
English |
description |
Abstract With the fast growth of today's aquaculture industry, the demand for aquafeeds is expanding dramatically. Insects, which are part of the natural diet of salmonids, could represent a sustainable ingredient for aquaculture feed. The aim of the current study was to test how a partial or total replacement of dietary fishmeal with insect meal affect gene responses involved in inflammation, the eicosanoid pathway and stress response in Atlantic salmon (Salmo salar L.) in isolated head kidney leukocytes after exposure to bacterial or viral mimic. Insect meal (IM) was produced from black soldier fly (BSF, Hermetia illucens) larvae. Seawater Atlantic salmon were fed three different diets for 8 weeks; a control diet (IM0, protein from fishmeal and plant based ingredients (25:75) and lipid from fish oil and vegetable oil (33:66); and two insect-meal containing diets, IM66 and IM100, where 66 and 100% of the fishmeal protein was replaced with IM, respectively. Leukocytes were isolated from the head kidney of fish (n = 6) from each of the three dietary groups. Isolated leukocytes were seeded into culture wells and added either a bacterial mimic (lipopolysaccharide, LPS) or a viral mimic (polyinosinic acid: polycytidylic acid, poly I: C) to induce an inflammatory response. Controls (Ctl) without LPS and poly I: C were included. The transcription of interleukins IL-1β, IL-8, IL-10 and TNF-α were elevated in LPS treated leukocytes isolated from salmon fed the three dietary groups (IM0, IM66 and IM100). The inflammatory-related gene expression in head kidney cells were, however, not affected by the pre-fed substitution of fish meal with IM in the diet of salmon. Gene transcriptions of PTGDS and PTGES were neither affected by LPS, poly I: C or the experimental diets fed prior to cell isolation, while salmon fed with IM showed a lower expression of LOX5. The gene expression of TLR22 and C/EBP-β were down-regulated by the LPS treatment in the cells isolated from salmon fed insect-based diets (IM66 and IM100) compared to ... |
format |
Article in Journal/Newspaper |
author |
Oda Kvalsvik Stenberg Elisabeth Holen Luisa Piemontese Nina S. Liland Erik-Jan Lock Marit Espe Ikram Belghit |
spellingShingle |
Oda Kvalsvik Stenberg Elisabeth Holen Luisa Piemontese Nina S. Liland Erik-Jan Lock Marit Espe Ikram Belghit Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes |
author_facet |
Oda Kvalsvik Stenberg Elisabeth Holen Luisa Piemontese Nina S. Liland Erik-Jan Lock Marit Espe Ikram Belghit |
author_sort |
Oda Kvalsvik Stenberg |
title |
Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes |
title_short |
Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes |
title_full |
Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes |
title_fullStr |
Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes |
title_full_unstemmed |
Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes |
title_sort |
effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in atlantic salmon (salmo salar) head kidney leukocytes |
publishDate |
2019 |
url |
https://www.openaccessrepository.it/record/35898 https://doi.org/10.1016/j.fsi.2019.05.042 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/35898 doi:10.1016/j.fsi.2019.05.042 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.fsi.2019.05.042 |
container_title |
Fish & Shellfish Immunology |
container_volume |
91 |
container_start_page |
223 |
op_container_end_page |
232 |
_version_ |
1766361972570324992 |