A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species
This study developed a simple, specific, and affordable PCR-sequencing-COI gene-based protocol for the simultaneous identification of some important commercial fish species: Merluccius merluccius, Lates niloticus, Gadus morhua, Ruvettus pretiosus, Pangasianodon hypophthalmus, Epinephelus spp. For th...
Published in: | Potravinarstvo Slovak Journal of Food Sciences |
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Online Access: | https://www.openaccessrepository.it/record/131953 https://doi.org/10.5219/1777 |
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ftopenaccessrep:oai:zenodo.org:131953 2023-10-29T02:36:26+01:00 A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species Ashraf Ali Pietro Di Taranto Antonio Parisi Laura Del Sambro Antonio Iannacci Donatella Belluscio Daniela Paola Debernardis Giovanni Normanno 2022-10-12 https://www.openaccessrepository.it/record/131953 https://doi.org/10.5219/1777 und unknown url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/131953 doi:10.5219/1777 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ Food Science info:eu-repo/semantics/article publication-article 2022 ftopenaccessrep https://doi.org/10.5219/1777 2023-10-03T22:18:28Z This study developed a simple, specific, and affordable PCR-sequencing-COI gene-based protocol for the simultaneous identification of some important commercial fish species: Merluccius merluccius, Lates niloticus, Gadus morhua, Ruvettus pretiosus, Pangasianodon hypophthalmus, Epinephelus spp. For this study, a local market survey on fish was carried out to evaluate the application of labelling laws and to detect fraudulent actions using the developed PCR protocols. Ten specimens of each fish species of interest were obtained from wholesale fishery plants and were utilized for the protocol development. DNA was extracted from the individual samples and quantified. DNA isolates were subjected to end-point PCR and the PCR products were sequenced. For the identification of fish species, novel species-specific primers were developed by the program "Primer Express 3.0" and by the software "Primer-BLAST" to amplify fragments of 200 bp, 250 bp, 300 and 562 bp, 350 bp, 400 bp and 522 bp within the COI gene for M. merluccius, L. niloticus, G. morhua, R. pretiosus, P. hypophthalmus, Epinephelus spp., respectively. Single PCR was performed using DNA isolates and developed primers for each fish species of interest. After sequencing, the isolates were compared with the selected sequences of the COI gene and showed a similarity ranging from 99 to 100%. Among 43 samples obtained for the survey, 19 (44.2%) were mislabelled, with 18 (41.9%) mislabelled samples from local fisheries and fish marketplaces and 1 (2.32%) from hypermarket stores. Among fish samples purchased at local fisheries and fish marketplaces, fraudulent actions were observed more frequently in fish slices (100%) than fish fillets (65%). Regarding fish fillets, out of four samples labelled as grouper, three were L. niloticus and one P. hypophthalmus. Two fillets marketed as cod were substituted with L. niloticus. Five samples labelled as "fillet" and two samples labelled as "perch" were identified as P. hypophthalmus. Regarding fish slices, all samples marketed as ... Article in Journal/Newspaper Gadus morhua Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository Potravinarstvo Slovak Journal of Food Sciences 16 656 669 |
institution |
Open Polar |
collection |
Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository |
op_collection_id |
ftopenaccessrep |
language |
unknown |
topic |
Food Science |
spellingShingle |
Food Science Ashraf Ali Pietro Di Taranto Antonio Parisi Laura Del Sambro Antonio Iannacci Donatella Belluscio Daniela Paola Debernardis Giovanni Normanno A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
topic_facet |
Food Science |
description |
This study developed a simple, specific, and affordable PCR-sequencing-COI gene-based protocol for the simultaneous identification of some important commercial fish species: Merluccius merluccius, Lates niloticus, Gadus morhua, Ruvettus pretiosus, Pangasianodon hypophthalmus, Epinephelus spp. For this study, a local market survey on fish was carried out to evaluate the application of labelling laws and to detect fraudulent actions using the developed PCR protocols. Ten specimens of each fish species of interest were obtained from wholesale fishery plants and were utilized for the protocol development. DNA was extracted from the individual samples and quantified. DNA isolates were subjected to end-point PCR and the PCR products were sequenced. For the identification of fish species, novel species-specific primers were developed by the program "Primer Express 3.0" and by the software "Primer-BLAST" to amplify fragments of 200 bp, 250 bp, 300 and 562 bp, 350 bp, 400 bp and 522 bp within the COI gene for M. merluccius, L. niloticus, G. morhua, R. pretiosus, P. hypophthalmus, Epinephelus spp., respectively. Single PCR was performed using DNA isolates and developed primers for each fish species of interest. After sequencing, the isolates were compared with the selected sequences of the COI gene and showed a similarity ranging from 99 to 100%. Among 43 samples obtained for the survey, 19 (44.2%) were mislabelled, with 18 (41.9%) mislabelled samples from local fisheries and fish marketplaces and 1 (2.32%) from hypermarket stores. Among fish samples purchased at local fisheries and fish marketplaces, fraudulent actions were observed more frequently in fish slices (100%) than fish fillets (65%). Regarding fish fillets, out of four samples labelled as grouper, three were L. niloticus and one P. hypophthalmus. Two fillets marketed as cod were substituted with L. niloticus. Five samples labelled as "fillet" and two samples labelled as "perch" were identified as P. hypophthalmus. Regarding fish slices, all samples marketed as ... |
format |
Article in Journal/Newspaper |
author |
Ashraf Ali Pietro Di Taranto Antonio Parisi Laura Del Sambro Antonio Iannacci Donatella Belluscio Daniela Paola Debernardis Giovanni Normanno |
author_facet |
Ashraf Ali Pietro Di Taranto Antonio Parisi Laura Del Sambro Antonio Iannacci Donatella Belluscio Daniela Paola Debernardis Giovanni Normanno |
author_sort |
Ashraf Ali |
title |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_short |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_full |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_fullStr |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_full_unstemmed |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_sort |
fish market survey using a novel pcr-sequencing-based protocols for the identification of commercial significant fish species |
publishDate |
2022 |
url |
https://www.openaccessrepository.it/record/131953 https://doi.org/10.5219/1777 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_relation |
url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/131953 doi:10.5219/1777 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.5219/1777 |
container_title |
Potravinarstvo Slovak Journal of Food Sciences |
container_volume |
16 |
container_start_page |
656 |
op_container_end_page |
669 |
_version_ |
1781060383979601920 |