Detection of IncP replicon-specific regions in DNA from Antarctic microbiota
Abstract Plasmids capable of horizontal transfer contribute to the adaptability of bacteria, as they may provide genes that enable their hosts to cope with different selective pressures. Only limited information is available on plasmids from Antarctic habitats, and up until now surveys have only use...
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ftopenaccessrep:oai:zenodo.org:118481 2023-10-29T02:31:27+01:00 Detection of IncP replicon-specific regions in DNA from Antarctic microbiota Roberto Bargagli Tatiana Imperio Laura Marri 2007-07-17 https://www.openaccessrepository.it/record/118481 https://doi.org/10.2478/s11535-007-0025-y eng eng url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/118481 doi:10.2478/s11535-007-0025-y info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ General Agricultural and Biological Sciences General Immunology and Microbiology General Biochemistry Genetics and Molecular Biology General Neuroscience info:eu-repo/semantics/article publication-article 2007 ftopenaccessrep https://doi.org/10.2478/s11535-007-0025-y 2023-10-03T22:17:55Z Abstract Plasmids capable of horizontal transfer contribute to the adaptability of bacteria, as they may provide genes that enable their hosts to cope with different selective pressures. Only limited information is available on plasmids from Antarctic habitats, and up until now surveys have only used traditional methods of endogenous plasmid isolation. The method based on primer systems, designed on the basis of published sequences for plasmids from different incompatibility (Inc) groups, is appropriate to detect the replicon-specific regions of corresponding plasmids in cultured bacteria, or in total community DNA, which share sufficient DNA similarity with reference plasmids at the amplified regions. In this study, we applied broad-host-range plasmid-specific primers to DNA from microbial samples collected at six different locations in Northern Victoria Land (Antarctica). DNA preparations were used as targets for PCR (polymerase chain reaction) amplification with primers for the IncP (trfA2) and IncQ (oriV ) groups. PCR products were Southern blotted and hybridized with PCR-derived probes for trfA2 and oriV regions. This approach detected the occurrence of IncP-specific sequences in eight out of fifteen DNA samples, suggesting a gene-mobilizing capacity within the original habitats. Article in Journal/Newspaper Antarc* Antarctic Antarctica Victoria Land Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository Open Life Sciences 2 3 378 384 |
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Open Polar |
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Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository |
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ftopenaccessrep |
language |
English |
topic |
General Agricultural and Biological Sciences General Immunology and Microbiology General Biochemistry Genetics and Molecular Biology General Neuroscience |
spellingShingle |
General Agricultural and Biological Sciences General Immunology and Microbiology General Biochemistry Genetics and Molecular Biology General Neuroscience Roberto Bargagli Tatiana Imperio Laura Marri Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
topic_facet |
General Agricultural and Biological Sciences General Immunology and Microbiology General Biochemistry Genetics and Molecular Biology General Neuroscience |
description |
Abstract Plasmids capable of horizontal transfer contribute to the adaptability of bacteria, as they may provide genes that enable their hosts to cope with different selective pressures. Only limited information is available on plasmids from Antarctic habitats, and up until now surveys have only used traditional methods of endogenous plasmid isolation. The method based on primer systems, designed on the basis of published sequences for plasmids from different incompatibility (Inc) groups, is appropriate to detect the replicon-specific regions of corresponding plasmids in cultured bacteria, or in total community DNA, which share sufficient DNA similarity with reference plasmids at the amplified regions. In this study, we applied broad-host-range plasmid-specific primers to DNA from microbial samples collected at six different locations in Northern Victoria Land (Antarctica). DNA preparations were used as targets for PCR (polymerase chain reaction) amplification with primers for the IncP (trfA2) and IncQ (oriV ) groups. PCR products were Southern blotted and hybridized with PCR-derived probes for trfA2 and oriV regions. This approach detected the occurrence of IncP-specific sequences in eight out of fifteen DNA samples, suggesting a gene-mobilizing capacity within the original habitats. |
format |
Article in Journal/Newspaper |
author |
Roberto Bargagli Tatiana Imperio Laura Marri |
author_facet |
Roberto Bargagli Tatiana Imperio Laura Marri |
author_sort |
Roberto Bargagli |
title |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_short |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_full |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_fullStr |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_full_unstemmed |
Detection of IncP replicon-specific regions in DNA from Antarctic microbiota |
title_sort |
detection of incp replicon-specific regions in dna from antarctic microbiota |
publishDate |
2007 |
url |
https://www.openaccessrepository.it/record/118481 https://doi.org/10.2478/s11535-007-0025-y |
genre |
Antarc* Antarctic Antarctica Victoria Land |
genre_facet |
Antarc* Antarctic Antarctica Victoria Land |
op_relation |
url:https://www.openaccessrepository.it/communities/itmirror https://www.openaccessrepository.it/record/118481 doi:10.2478/s11535-007-0025-y |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.2478/s11535-007-0025-y |
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Open Life Sciences |
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2 |
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3 |
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378 |
op_container_end_page |
384 |
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1781069893700943872 |