Stable transmission and transcription of Newfoundland ocean pout type III fish antifreeze protein (AFP) gene in transgenic mice and hypothermic storage of transgenic ovary and testis
BAGIS/0000-0002-1140-8058 WOS: 000240661900007 PubMed: 16894545 Here we describe the generation of transgenic mice carrying type III fish antifreeze protein (AFP) gene and evaluate whether AFP type III protects transgenic mouse ovaries and testes from hypothermic storage. AFPs exist in many differen...
Published in: | Molecular Reproduction and Development |
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Online Access: | https://hdl.handle.net/20.500.12712/20366 https://doi.org/10.1002/mrd.20601 |
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ftondomayuniv:oai:acikerisim.omu.edu.tr:20.500.12712/20366 2023-05-15T17:21:54+02:00 Stable transmission and transcription of Newfoundland ocean pout type III fish antifreeze protein (AFP) gene in transgenic mice and hypothermic storage of transgenic ovary and testis Bagis, Haydar Aktoprakligil, Digdem Mercan, Hande Odaman Yurdusev, Nevzat Turgut, Gazi Sekmen, Sakir Cetin, Seyfettin Ondokuz Mayıs Üniversitesi 2006 https://hdl.handle.net/20.500.12712/20366 https://doi.org/10.1002/mrd.20601 eng eng Wiley-Liss 10.1002/mrd.20601 Molecular Reproduction and Development Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı 1040-452X https://doi.org/10.1002/mrd.20601 https://hdl.handle.net/20.500.12712/20366 73 11 1404 1411 info:eu-repo/semantics/closedAccess transgenic mouse antifreeze protein ocean pout hypothermic storage article 2006 ftondomayuniv https://doi.org/20.500.12712/20366 https://doi.org/10.1002/mrd.20601 2022-07-21T08:43:06Z BAGIS/0000-0002-1140-8058 WOS: 000240661900007 PubMed: 16894545 Here we describe the generation of transgenic mice carrying type III fish antifreeze protein (AFP) gene and evaluate whether AFP type III protects transgenic mouse ovaries and testes from hypothermic storage. AFPs exist in many different organisms. In fish, AFPs protect the host from freezing at temperatures below the colligative freezing point by adsorbing to the surface of nucleating ice crystals and inhibiting their growth. The transgenic expression of AFP holds great promise for conferring freeze-resistant plant and animal species. AFP also exhibits a potential for the cryopreservation of tissues and cells. In this study, we have generated 42 founder mice harboring the Newfoundland ocean pout (OP5A) type III AFP transgene and established one transgenic line (the line #6). This study demonstrated that AFP gene construct has been stably transmitted to the mouse progeny in the F3 generations in the line #6. Furthermore, the presence of AFP transcripts was confirmed by RT-PCR analysis on cDNAs from liver, kidney, ovarian, and testis tissues of the mouse from F3 generation in this line. These results indicate that ocean pout type III AFP gene could be integrated and transmitted to the next generation and stably transcribed in transgenic mice. In histological analysis of testis and ovarian tissues of nontransgenic control and AFP transgenic mice it has been shown that both tissues of AFP transgenic mice were protected from hypothermic storage (+4 degrees C). The AFP III transgenic mice obtained for the first time in this study would be useful for investigating the biological functions of AFP in mammalian systems and also its potential role in cryopreservation. Article in Journal/Newspaper Newfoundland Ondokuz Mayıs University Institutional Repository (DSpace@OMU) Molecular Reproduction and Development 73 11 1404 1411 |
institution |
Open Polar |
collection |
Ondokuz Mayıs University Institutional Repository (DSpace@OMU) |
op_collection_id |
ftondomayuniv |
language |
English |
topic |
transgenic mouse antifreeze protein ocean pout hypothermic storage |
spellingShingle |
transgenic mouse antifreeze protein ocean pout hypothermic storage Bagis, Haydar Aktoprakligil, Digdem Mercan, Hande Odaman Yurdusev, Nevzat Turgut, Gazi Sekmen, Sakir Cetin, Seyfettin Stable transmission and transcription of Newfoundland ocean pout type III fish antifreeze protein (AFP) gene in transgenic mice and hypothermic storage of transgenic ovary and testis |
topic_facet |
transgenic mouse antifreeze protein ocean pout hypothermic storage |
description |
BAGIS/0000-0002-1140-8058 WOS: 000240661900007 PubMed: 16894545 Here we describe the generation of transgenic mice carrying type III fish antifreeze protein (AFP) gene and evaluate whether AFP type III protects transgenic mouse ovaries and testes from hypothermic storage. AFPs exist in many different organisms. In fish, AFPs protect the host from freezing at temperatures below the colligative freezing point by adsorbing to the surface of nucleating ice crystals and inhibiting their growth. The transgenic expression of AFP holds great promise for conferring freeze-resistant plant and animal species. AFP also exhibits a potential for the cryopreservation of tissues and cells. In this study, we have generated 42 founder mice harboring the Newfoundland ocean pout (OP5A) type III AFP transgene and established one transgenic line (the line #6). This study demonstrated that AFP gene construct has been stably transmitted to the mouse progeny in the F3 generations in the line #6. Furthermore, the presence of AFP transcripts was confirmed by RT-PCR analysis on cDNAs from liver, kidney, ovarian, and testis tissues of the mouse from F3 generation in this line. These results indicate that ocean pout type III AFP gene could be integrated and transmitted to the next generation and stably transcribed in transgenic mice. In histological analysis of testis and ovarian tissues of nontransgenic control and AFP transgenic mice it has been shown that both tissues of AFP transgenic mice were protected from hypothermic storage (+4 degrees C). The AFP III transgenic mice obtained for the first time in this study would be useful for investigating the biological functions of AFP in mammalian systems and also its potential role in cryopreservation. |
author2 |
Ondokuz Mayıs Üniversitesi |
format |
Article in Journal/Newspaper |
author |
Bagis, Haydar Aktoprakligil, Digdem Mercan, Hande Odaman Yurdusev, Nevzat Turgut, Gazi Sekmen, Sakir Cetin, Seyfettin |
author_facet |
Bagis, Haydar Aktoprakligil, Digdem Mercan, Hande Odaman Yurdusev, Nevzat Turgut, Gazi Sekmen, Sakir Cetin, Seyfettin |
author_sort |
Bagis, Haydar |
title |
Stable transmission and transcription of Newfoundland ocean pout type III fish antifreeze protein (AFP) gene in transgenic mice and hypothermic storage of transgenic ovary and testis |
title_short |
Stable transmission and transcription of Newfoundland ocean pout type III fish antifreeze protein (AFP) gene in transgenic mice and hypothermic storage of transgenic ovary and testis |
title_full |
Stable transmission and transcription of Newfoundland ocean pout type III fish antifreeze protein (AFP) gene in transgenic mice and hypothermic storage of transgenic ovary and testis |
title_fullStr |
Stable transmission and transcription of Newfoundland ocean pout type III fish antifreeze protein (AFP) gene in transgenic mice and hypothermic storage of transgenic ovary and testis |
title_full_unstemmed |
Stable transmission and transcription of Newfoundland ocean pout type III fish antifreeze protein (AFP) gene in transgenic mice and hypothermic storage of transgenic ovary and testis |
title_sort |
stable transmission and transcription of newfoundland ocean pout type iii fish antifreeze protein (afp) gene in transgenic mice and hypothermic storage of transgenic ovary and testis |
publisher |
Wiley-Liss |
publishDate |
2006 |
url |
https://hdl.handle.net/20.500.12712/20366 https://doi.org/10.1002/mrd.20601 |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_relation |
10.1002/mrd.20601 Molecular Reproduction and Development Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı 1040-452X https://doi.org/10.1002/mrd.20601 https://hdl.handle.net/20.500.12712/20366 73 11 1404 1411 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/20.500.12712/20366 https://doi.org/10.1002/mrd.20601 |
container_title |
Molecular Reproduction and Development |
container_volume |
73 |
container_issue |
11 |
container_start_page |
1404 |
op_container_end_page |
1411 |
_version_ |
1766107898541244416 |