Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey
Ozan, Emre/0000-0001-9872-8152; ISIDAN, Hakan/0000-0002-5080-1936 WOS: 000424211900006 Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economica...
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ftondomayuniv:oai:acikerisim.omu.edu.tr:20.500.12712/12048 2023-05-15T18:15:53+02:00 Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey Albayrak, H. Isidan, H. Kalayci, G. Ozan, E. Vakharia, V. N. Ondokuz Mayıs Üniversitesi 2018 https://hdl.handle.net/20.500.12712/12048 eng eng Iranian Fisheries Science Research Inst-Ifsri Iranian Journal of Fisheries Sciences Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı 1562-2916 https://hdl.handle.net/20.500.12712/12048 17 1 67 73 info:eu-repo/semantics/closedAccess Genotype Rainbow trout Turbot Turkey VHSV article 2018 ftondomayuniv https://doi.org/20.500.12712/12048 2022-07-21T08:40:18Z Ozan, Emre/0000-0001-9872-8152; ISIDAN, Hakan/0000-0002-5080-1936 WOS: 000424211900006 Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases in farmed rainbow trout, in Turkey, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout (Oncorhynchus mykiss) samples were collected during an outbreak of VHSV in a rainbow trout fish farm in Bolu Province of Turkey in 2006. In addition, two VHSV strains were isolated from wild turbot (Scophthalmus maximus) in Trabzon Province of the Black Sea region of Turkey during a field survey. We have sequenced the full-length glycoprotein (G) gene of three VHSV isolates and compared them with 25 previously published gene sequences. Based on a complete gene nucleotide sequence, Turkish VHSV isolates were classified into class Ie of genotype I, which is closely related to GE-1.2 isolate (97.1-97.5% nucleotide identity and 98.2-98.4% amino acid identity) found in Georgia more than 30 years ago. These isolates could be an indigenous type of VHSV distributed in the Black Sea. On the other hand, Turkish isolates have 97.5-97.6% nucleotide identity and 98.8-99% amino acid identity with Finnish, Danish, and Norwegian isolates which are classified under Ib and Id. These results suggest that Turkish VHSV isolates may have orginated from Europe and co-circulated with indigenous strains which can threaten the aquaculture industry in Turkey. Samsun Veterinary Control Institute (SVCRI) We are grateful to Allison J. May (The University of Texas, Austin, TX, USA) for critically reading the manuscript. Funding for this research was provided by the Samsun Veterinary Control Institute (SVCRI) general budget. Article in Journal/Newspaper Scophthalmus maximus Turbot Ondokuz Mayıs University Institutional Repository (DSpace@OMU) Austin |
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Ondokuz Mayıs University Institutional Repository (DSpace@OMU) |
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ftondomayuniv |
language |
English |
topic |
Genotype Rainbow trout Turbot Turkey VHSV |
spellingShingle |
Genotype Rainbow trout Turbot Turkey VHSV Albayrak, H. Isidan, H. Kalayci, G. Ozan, E. Vakharia, V. N. Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
topic_facet |
Genotype Rainbow trout Turbot Turkey VHSV |
description |
Ozan, Emre/0000-0001-9872-8152; ISIDAN, Hakan/0000-0002-5080-1936 WOS: 000424211900006 Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases in farmed rainbow trout, in Turkey, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout (Oncorhynchus mykiss) samples were collected during an outbreak of VHSV in a rainbow trout fish farm in Bolu Province of Turkey in 2006. In addition, two VHSV strains were isolated from wild turbot (Scophthalmus maximus) in Trabzon Province of the Black Sea region of Turkey during a field survey. We have sequenced the full-length glycoprotein (G) gene of three VHSV isolates and compared them with 25 previously published gene sequences. Based on a complete gene nucleotide sequence, Turkish VHSV isolates were classified into class Ie of genotype I, which is closely related to GE-1.2 isolate (97.1-97.5% nucleotide identity and 98.2-98.4% amino acid identity) found in Georgia more than 30 years ago. These isolates could be an indigenous type of VHSV distributed in the Black Sea. On the other hand, Turkish isolates have 97.5-97.6% nucleotide identity and 98.8-99% amino acid identity with Finnish, Danish, and Norwegian isolates which are classified under Ib and Id. These results suggest that Turkish VHSV isolates may have orginated from Europe and co-circulated with indigenous strains which can threaten the aquaculture industry in Turkey. Samsun Veterinary Control Institute (SVCRI) We are grateful to Allison J. May (The University of Texas, Austin, TX, USA) for critically reading the manuscript. Funding for this research was provided by the Samsun Veterinary Control Institute (SVCRI) general budget. |
author2 |
Ondokuz Mayıs Üniversitesi |
format |
Article in Journal/Newspaper |
author |
Albayrak, H. Isidan, H. Kalayci, G. Ozan, E. Vakharia, V. N. |
author_facet |
Albayrak, H. Isidan, H. Kalayci, G. Ozan, E. Vakharia, V. N. |
author_sort |
Albayrak, H. |
title |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_short |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_full |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_fullStr |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_full_unstemmed |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_sort |
genetic analysis of the complete g gene of viral hemorrhagic septicemia virus (vhsv) genotype ie isolates from turkey |
publisher |
Iranian Fisheries Science Research Inst-Ifsri |
publishDate |
2018 |
url |
https://hdl.handle.net/20.500.12712/12048 |
geographic |
Austin |
geographic_facet |
Austin |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
Iranian Journal of Fisheries Sciences Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı 1562-2916 https://hdl.handle.net/20.500.12712/12048 17 1 67 73 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/20.500.12712/12048 |
_version_ |
1766189133853622272 |