Metaproteomics Reveal That Rapid Perturbations in Organic Matter Prioritize Functional Restructuring Over Taxonomy In Western Arctic Ocean Microbiomes
We examined metaproteome profiles from two Arctic microbiomes during 10-day shipboard incubations to directly track early functional and taxonomic responses to a simulated algal bloom and an oligotrophic control. Using a novel peptide-based enrichment analysis, significant changes (p-value < 0.01...
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ODU Digital Commons
2019
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Online Access: | https://digitalcommons.odu.edu/oeas_fac_pubs/360 https://doi.org/10.1038/s41396-019-0503-z https://digitalcommons.odu.edu/context/oeas_fac_pubs/article/1371/viewcontent/Harvey_2019_MetaproteomicsRevealThatRapidPerturbationsinOrganicMatterOCR.pdf |
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ftolddominionuni:oai:digitalcommons.odu.edu:oeas_fac_pubs-1371 2023-06-11T04:08:51+02:00 Metaproteomics Reveal That Rapid Perturbations in Organic Matter Prioritize Functional Restructuring Over Taxonomy In Western Arctic Ocean Microbiomes Mikan, Molly P. Harvey, H. Rodger Timmins-Schiffman, Emma Riffle, Michael May, Damon H. Salter, Ian Noble, William S. Nunn, Brook L. 2019-01-01T08:00:00Z application/pdf https://digitalcommons.odu.edu/oeas_fac_pubs/360 https://doi.org/10.1038/s41396-019-0503-z https://digitalcommons.odu.edu/context/oeas_fac_pubs/article/1371/viewcontent/Harvey_2019_MetaproteomicsRevealThatRapidPerturbationsinOrganicMatterOCR.pdf unknown ODU Digital Commons https://digitalcommons.odu.edu/oeas_fac_pubs/360 doi:10.1038/s41396-019-0503-z https://digitalcommons.odu.edu/context/oeas_fac_pubs/article/1371/viewcontent/Harvey_2019_MetaproteomicsRevealThatRapidPerturbationsinOrganicMatterOCR.pdf Published under a Creative Commons Attribution 4.0 International License . OES Faculty Publications Marine microbiology Microbial biooceanography Microbial ecology Proteomics Microbiology Oceanography Organic Chemistry article 2019 ftolddominionuni https://doi.org/10.1038/s41396-019-0503-z 2023-05-08T18:00:50Z We examined metaproteome profiles from two Arctic microbiomes during 10-day shipboard incubations to directly track early functional and taxonomic responses to a simulated algal bloom and an oligotrophic control. Using a novel peptide-based enrichment analysis, significant changes (p-value < 0.01) in biological and molecular functions associated with carbon and nitrogen recycling were observed. Within the first day under both organic matter conditions, Bering Strait surface microbiomes increased protein synthesis, carbohydrate degradation, and cellular redox processes while decreasing C1 metabolism. Taxonomic assignments revealed that the core microbiome collectively responded to algal substrates by assimilating carbon before select taxa utilize and metabolize nitrogen intracellularly. Incubations of Chukchi Sea bottom water microbiomes showed similar, but delayed functional responses to identical treatments. Although 24 functional terms were shared between experimental treatments, the timing, and degree of the remaining responses were highly variable, showing that organic matter perturbation directs community functionality prior to alterations to the taxonomic distribution at the microbiome class level. The dynamic responses of these two oceanic microbial communities have important implications for timing and magnitude of responses to organic perturbations within the Arctic Ocean and how community-level functions may forecast biogeochemical gradients in oceans. Article in Journal/Newspaper Arctic Arctic Ocean Bering Strait Chukchi Chukchi Sea Old Dominion University: ODU Digital Commons Arctic Arctic Ocean Chukchi Sea Bering Strait The ISME Journal 14 1 39 52 |
institution |
Open Polar |
collection |
Old Dominion University: ODU Digital Commons |
op_collection_id |
ftolddominionuni |
language |
unknown |
topic |
Marine microbiology Microbial biooceanography Microbial ecology Proteomics Microbiology Oceanography Organic Chemistry |
spellingShingle |
Marine microbiology Microbial biooceanography Microbial ecology Proteomics Microbiology Oceanography Organic Chemistry Mikan, Molly P. Harvey, H. Rodger Timmins-Schiffman, Emma Riffle, Michael May, Damon H. Salter, Ian Noble, William S. Nunn, Brook L. Metaproteomics Reveal That Rapid Perturbations in Organic Matter Prioritize Functional Restructuring Over Taxonomy In Western Arctic Ocean Microbiomes |
topic_facet |
Marine microbiology Microbial biooceanography Microbial ecology Proteomics Microbiology Oceanography Organic Chemistry |
description |
We examined metaproteome profiles from two Arctic microbiomes during 10-day shipboard incubations to directly track early functional and taxonomic responses to a simulated algal bloom and an oligotrophic control. Using a novel peptide-based enrichment analysis, significant changes (p-value < 0.01) in biological and molecular functions associated with carbon and nitrogen recycling were observed. Within the first day under both organic matter conditions, Bering Strait surface microbiomes increased protein synthesis, carbohydrate degradation, and cellular redox processes while decreasing C1 metabolism. Taxonomic assignments revealed that the core microbiome collectively responded to algal substrates by assimilating carbon before select taxa utilize and metabolize nitrogen intracellularly. Incubations of Chukchi Sea bottom water microbiomes showed similar, but delayed functional responses to identical treatments. Although 24 functional terms were shared between experimental treatments, the timing, and degree of the remaining responses were highly variable, showing that organic matter perturbation directs community functionality prior to alterations to the taxonomic distribution at the microbiome class level. The dynamic responses of these two oceanic microbial communities have important implications for timing and magnitude of responses to organic perturbations within the Arctic Ocean and how community-level functions may forecast biogeochemical gradients in oceans. |
format |
Article in Journal/Newspaper |
author |
Mikan, Molly P. Harvey, H. Rodger Timmins-Schiffman, Emma Riffle, Michael May, Damon H. Salter, Ian Noble, William S. Nunn, Brook L. |
author_facet |
Mikan, Molly P. Harvey, H. Rodger Timmins-Schiffman, Emma Riffle, Michael May, Damon H. Salter, Ian Noble, William S. Nunn, Brook L. |
author_sort |
Mikan, Molly P. |
title |
Metaproteomics Reveal That Rapid Perturbations in Organic Matter Prioritize Functional Restructuring Over Taxonomy In Western Arctic Ocean Microbiomes |
title_short |
Metaproteomics Reveal That Rapid Perturbations in Organic Matter Prioritize Functional Restructuring Over Taxonomy In Western Arctic Ocean Microbiomes |
title_full |
Metaproteomics Reveal That Rapid Perturbations in Organic Matter Prioritize Functional Restructuring Over Taxonomy In Western Arctic Ocean Microbiomes |
title_fullStr |
Metaproteomics Reveal That Rapid Perturbations in Organic Matter Prioritize Functional Restructuring Over Taxonomy In Western Arctic Ocean Microbiomes |
title_full_unstemmed |
Metaproteomics Reveal That Rapid Perturbations in Organic Matter Prioritize Functional Restructuring Over Taxonomy In Western Arctic Ocean Microbiomes |
title_sort |
metaproteomics reveal that rapid perturbations in organic matter prioritize functional restructuring over taxonomy in western arctic ocean microbiomes |
publisher |
ODU Digital Commons |
publishDate |
2019 |
url |
https://digitalcommons.odu.edu/oeas_fac_pubs/360 https://doi.org/10.1038/s41396-019-0503-z https://digitalcommons.odu.edu/context/oeas_fac_pubs/article/1371/viewcontent/Harvey_2019_MetaproteomicsRevealThatRapidPerturbationsinOrganicMatterOCR.pdf |
geographic |
Arctic Arctic Ocean Chukchi Sea Bering Strait |
geographic_facet |
Arctic Arctic Ocean Chukchi Sea Bering Strait |
genre |
Arctic Arctic Ocean Bering Strait Chukchi Chukchi Sea |
genre_facet |
Arctic Arctic Ocean Bering Strait Chukchi Chukchi Sea |
op_source |
OES Faculty Publications |
op_relation |
https://digitalcommons.odu.edu/oeas_fac_pubs/360 doi:10.1038/s41396-019-0503-z https://digitalcommons.odu.edu/context/oeas_fac_pubs/article/1371/viewcontent/Harvey_2019_MetaproteomicsRevealThatRapidPerturbationsinOrganicMatterOCR.pdf |
op_rights |
Published under a Creative Commons Attribution 4.0 International License . |
op_doi |
https://doi.org/10.1038/s41396-019-0503-z |
container_title |
The ISME Journal |
container_volume |
14 |
container_issue |
1 |
container_start_page |
39 |
op_container_end_page |
52 |
_version_ |
1768382435044622336 |