Predicting Carbon Isotope Discrimination in Eelgrass (Zostera marina L.) From the Environmental Parameters- Light, Flow, and [DIC]
Isotopic discrimination against 13C during photosynthesis is determined by a combination of environmental conditions and physiological mechanisms that control delivery of CO2 to RUBISCO. This study investigated the effects of light, flow, dissolved inorganic carbon (DIC) concentration, and its speci...
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ftolddominionuni:oai:digitalcommons.odu.edu:oeas_fac_pubs-1086 2024-04-07T07:55:10+00:00 Predicting Carbon Isotope Discrimination in Eelgrass (Zostera marina L.) From the Environmental Parameters- Light, Flow, and [DIC] McPherson, Meredith L. Zimmerman, Richard C. Hill, Victoria J. 2015-01-01T08:00:00Z application/pdf https://digitalcommons.odu.edu/oeas_fac_pubs/110 https://doi.org/10.1002/lno.10142 https://digitalcommons.odu.edu/context/oeas_fac_pubs/article/1086/viewcontent/McPherson_2015_Predicting_carbon_is.pdf unknown ODU Digital Commons https://digitalcommons.odu.edu/oeas_fac_pubs/110 doi:10.1002/lno.10142 https://digitalcommons.odu.edu/context/oeas_fac_pubs/article/1086/viewcontent/McPherson_2015_Predicting_carbon_is.pdf OES Faculty Publications Seagrass Thalassia testudinum Inorganic carbon CO2 Enrichment Bicarbonate utilization Aquatic macrophytes Posidonia oceanica Light intensity South Florida Photosynthesis Variability Marine Biology Oceanography Plant Biology article 2015 ftolddominionuni https://doi.org/10.1002/lno.10142 2024-03-14T17:51:17Z Isotopic discrimination against 13C during photosynthesis is determined by a combination of environmental conditions and physiological mechanisms that control delivery of CO2 to RUBISCO. This study investigated the effects of light, flow, dissolved inorganic carbon (DIC) concentration, and its speciation, on photosynthetic carbon assimilation of Zostera marinaL. (eelgrass) using a combination of laboratory experiments and theoretical calculations leading to a mechanistic understanding of environmental conditions that influence leaf carbon uptake and determine leaf stable carbon isotope signatures δ13C. Photosynthesis was saturated with respect to flow at low velocity ~ 3 cm s-1, but was strongly influenced by [DIC], and particularly aqueous CO2 (CO2(aq)) under all flow conditions. The non-linear responses of light- and flow-saturated photosynthesis to [DIC] were used to quantify the maximum physiological capacity for photosynthesis, and to determine the degree of photosynthetic carbon limitation for light-saturated photosynthesis, which provided a mechanistic pathway for modeling regulation of carbon uptake and 13C discrimination. Model predictions of δ13C spanned the typical range of values reported for a variety of seagrass taxa, and were most sensitive to [DIC] (predominantly [CO2(aq) ]) and flow, but less sensitive to DIC source [CO2(aq)vs. HCO¯3]. These results provide a predictive understanding of the role of key environmental parameters (light, flow, and DIC availability) can have in driving δ13C of seagrasses, which will become increasingly important for predicting the response of these ecosystem engineers to local processes that affect light availability and flow, as well as global impacts of climate warming and ocean acidification in the Anthropocene. Article in Journal/Newspaper Ocean acidification Old Dominion University: ODU Digital Commons Limnology and Oceanography 60 6 1875 1889 |
institution |
Open Polar |
collection |
Old Dominion University: ODU Digital Commons |
op_collection_id |
ftolddominionuni |
language |
unknown |
topic |
Seagrass Thalassia testudinum Inorganic carbon CO2 Enrichment Bicarbonate utilization Aquatic macrophytes Posidonia oceanica Light intensity South Florida Photosynthesis Variability Marine Biology Oceanography Plant Biology |
spellingShingle |
Seagrass Thalassia testudinum Inorganic carbon CO2 Enrichment Bicarbonate utilization Aquatic macrophytes Posidonia oceanica Light intensity South Florida Photosynthesis Variability Marine Biology Oceanography Plant Biology McPherson, Meredith L. Zimmerman, Richard C. Hill, Victoria J. Predicting Carbon Isotope Discrimination in Eelgrass (Zostera marina L.) From the Environmental Parameters- Light, Flow, and [DIC] |
topic_facet |
Seagrass Thalassia testudinum Inorganic carbon CO2 Enrichment Bicarbonate utilization Aquatic macrophytes Posidonia oceanica Light intensity South Florida Photosynthesis Variability Marine Biology Oceanography Plant Biology |
description |
Isotopic discrimination against 13C during photosynthesis is determined by a combination of environmental conditions and physiological mechanisms that control delivery of CO2 to RUBISCO. This study investigated the effects of light, flow, dissolved inorganic carbon (DIC) concentration, and its speciation, on photosynthetic carbon assimilation of Zostera marinaL. (eelgrass) using a combination of laboratory experiments and theoretical calculations leading to a mechanistic understanding of environmental conditions that influence leaf carbon uptake and determine leaf stable carbon isotope signatures δ13C. Photosynthesis was saturated with respect to flow at low velocity ~ 3 cm s-1, but was strongly influenced by [DIC], and particularly aqueous CO2 (CO2(aq)) under all flow conditions. The non-linear responses of light- and flow-saturated photosynthesis to [DIC] were used to quantify the maximum physiological capacity for photosynthesis, and to determine the degree of photosynthetic carbon limitation for light-saturated photosynthesis, which provided a mechanistic pathway for modeling regulation of carbon uptake and 13C discrimination. Model predictions of δ13C spanned the typical range of values reported for a variety of seagrass taxa, and were most sensitive to [DIC] (predominantly [CO2(aq) ]) and flow, but less sensitive to DIC source [CO2(aq)vs. HCO¯3]. These results provide a predictive understanding of the role of key environmental parameters (light, flow, and DIC availability) can have in driving δ13C of seagrasses, which will become increasingly important for predicting the response of these ecosystem engineers to local processes that affect light availability and flow, as well as global impacts of climate warming and ocean acidification in the Anthropocene. |
format |
Article in Journal/Newspaper |
author |
McPherson, Meredith L. Zimmerman, Richard C. Hill, Victoria J. |
author_facet |
McPherson, Meredith L. Zimmerman, Richard C. Hill, Victoria J. |
author_sort |
McPherson, Meredith L. |
title |
Predicting Carbon Isotope Discrimination in Eelgrass (Zostera marina L.) From the Environmental Parameters- Light, Flow, and [DIC] |
title_short |
Predicting Carbon Isotope Discrimination in Eelgrass (Zostera marina L.) From the Environmental Parameters- Light, Flow, and [DIC] |
title_full |
Predicting Carbon Isotope Discrimination in Eelgrass (Zostera marina L.) From the Environmental Parameters- Light, Flow, and [DIC] |
title_fullStr |
Predicting Carbon Isotope Discrimination in Eelgrass (Zostera marina L.) From the Environmental Parameters- Light, Flow, and [DIC] |
title_full_unstemmed |
Predicting Carbon Isotope Discrimination in Eelgrass (Zostera marina L.) From the Environmental Parameters- Light, Flow, and [DIC] |
title_sort |
predicting carbon isotope discrimination in eelgrass (zostera marina l.) from the environmental parameters- light, flow, and [dic] |
publisher |
ODU Digital Commons |
publishDate |
2015 |
url |
https://digitalcommons.odu.edu/oeas_fac_pubs/110 https://doi.org/10.1002/lno.10142 https://digitalcommons.odu.edu/context/oeas_fac_pubs/article/1086/viewcontent/McPherson_2015_Predicting_carbon_is.pdf |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
OES Faculty Publications |
op_relation |
https://digitalcommons.odu.edu/oeas_fac_pubs/110 doi:10.1002/lno.10142 https://digitalcommons.odu.edu/context/oeas_fac_pubs/article/1086/viewcontent/McPherson_2015_Predicting_carbon_is.pdf |
op_doi |
https://doi.org/10.1002/lno.10142 |
container_title |
Limnology and Oceanography |
container_volume |
60 |
container_issue |
6 |
container_start_page |
1875 |
op_container_end_page |
1889 |
_version_ |
1795672142809923584 |