Spectroscopic Characterization of Oceanic Dissolved Organic Matter Isolated By Reverse Osmosis Coupled With Electrodialysis
Oceanic dissolved organic matter (DOM) is one of the largest pools of reduced carbon on Earth, yet DOM remains poorly chemically characterized. Studies to determine the chemical nature of oceanic DOM have been impeded by the lack of efficient and non-fractioning methods to recover oceanic DOM. Here,...
Main Authors: | , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
ODU Digital Commons
2015
|
Subjects: | |
Online Access: | https://digitalcommons.odu.edu/chemistry_fac_pubs/148 https://digitalcommons.odu.edu/cgi/viewcontent.cgi?article=1151&context=chemistry_fac_pubs |
id |
ftolddominionuni:oai:digitalcommons.odu.edu:chemistry_fac_pubs-1151 |
---|---|
record_format |
openpolar |
spelling |
ftolddominionuni:oai:digitalcommons.odu.edu:chemistry_fac_pubs-1151 2023-05-15T17:25:26+02:00 Spectroscopic Characterization of Oceanic Dissolved Organic Matter Isolated By Reverse Osmosis Coupled With Electrodialysis Helms, John R. Mao, Jingdong Chen, Hongmei Perdue, E. Michael Green, Nelson W. Hatcher, Patrick G. Mopper, Kenneth Stubbins, Aron 2015-01-01T08:00:00Z application/pdf https://digitalcommons.odu.edu/chemistry_fac_pubs/148 https://digitalcommons.odu.edu/cgi/viewcontent.cgi?article=1151&context=chemistry_fac_pubs unknown ODU Digital Commons https://digitalcommons.odu.edu/chemistry_fac_pubs/148 https://digitalcommons.odu.edu/cgi/viewcontent.cgi?article=1151&context=chemistry_fac_pubs Chemistry & Biochemistry Faculty Publications Dissolved organic matter Carbon cycle Nuclear magnetic resonance Reverse osmosis-electrodialysis (RO/ED) Biogeochemistry Chemistry Oceanography article 2015 ftolddominionuni 2021-03-02T18:17:10Z Oceanic dissolved organic matter (DOM) is one of the largest pools of reduced carbon on Earth, yet DOM remains poorly chemically characterized. Studies to determine the chemical nature of oceanic DOM have been impeded by the lack of efficient and non-fractioning methods to recover oceanic DOM. Here, a DOM fraction (~40 to 86% recovery) was isolated using reverse osmosis/electrodialysis (RO/ED) and analyzed by solid-state 13C nuclear magnetic resonance (NMR) spectroscopy. Samples were obtained from biogeochemically distinct environments: photobleached surface gyre, productive coastal upwelling zone, oxygen minimum, North Atlantic Deep Water, and North Pacific Deep Water. A ubiquitous ‘background’ refractory carbon pool was apparent throughout the ocean and dominated in the deep Pacific Ocean. Advanced NMR spectral editing revealed that condensed aromatic and quaternary anomeric carbons contribute to this deep refractory DOC pool, the quaternary anomeric carbons being a newly identified and potentially important component of bio-refractory carbohydrate-like carbon. Additionally, our results support the multi-pool (e.g. 3-pool: labile, semi-labile, and refractory) conceptual model of marine DOM biogeochemistry. Surface samples, hypothesized to be enriched in labile and semi-labile DOM, were enriched in carbohydrate-like material consistent with prior studies. High carboxyl signals in the deep Pacific support the hypothesis that a major fraction of the refractory pool consists of carboxyl-rich alicyclic molecules (CRAM). Article in Journal/Newspaper North Atlantic Deep Water North Atlantic Old Dominion University: ODU Digital Commons Pacific |
institution |
Open Polar |
collection |
Old Dominion University: ODU Digital Commons |
op_collection_id |
ftolddominionuni |
language |
unknown |
topic |
Dissolved organic matter Carbon cycle Nuclear magnetic resonance Reverse osmosis-electrodialysis (RO/ED) Biogeochemistry Chemistry Oceanography |
spellingShingle |
Dissolved organic matter Carbon cycle Nuclear magnetic resonance Reverse osmosis-electrodialysis (RO/ED) Biogeochemistry Chemistry Oceanography Helms, John R. Mao, Jingdong Chen, Hongmei Perdue, E. Michael Green, Nelson W. Hatcher, Patrick G. Mopper, Kenneth Stubbins, Aron Spectroscopic Characterization of Oceanic Dissolved Organic Matter Isolated By Reverse Osmosis Coupled With Electrodialysis |
topic_facet |
Dissolved organic matter Carbon cycle Nuclear magnetic resonance Reverse osmosis-electrodialysis (RO/ED) Biogeochemistry Chemistry Oceanography |
description |
Oceanic dissolved organic matter (DOM) is one of the largest pools of reduced carbon on Earth, yet DOM remains poorly chemically characterized. Studies to determine the chemical nature of oceanic DOM have been impeded by the lack of efficient and non-fractioning methods to recover oceanic DOM. Here, a DOM fraction (~40 to 86% recovery) was isolated using reverse osmosis/electrodialysis (RO/ED) and analyzed by solid-state 13C nuclear magnetic resonance (NMR) spectroscopy. Samples were obtained from biogeochemically distinct environments: photobleached surface gyre, productive coastal upwelling zone, oxygen minimum, North Atlantic Deep Water, and North Pacific Deep Water. A ubiquitous ‘background’ refractory carbon pool was apparent throughout the ocean and dominated in the deep Pacific Ocean. Advanced NMR spectral editing revealed that condensed aromatic and quaternary anomeric carbons contribute to this deep refractory DOC pool, the quaternary anomeric carbons being a newly identified and potentially important component of bio-refractory carbohydrate-like carbon. Additionally, our results support the multi-pool (e.g. 3-pool: labile, semi-labile, and refractory) conceptual model of marine DOM biogeochemistry. Surface samples, hypothesized to be enriched in labile and semi-labile DOM, were enriched in carbohydrate-like material consistent with prior studies. High carboxyl signals in the deep Pacific support the hypothesis that a major fraction of the refractory pool consists of carboxyl-rich alicyclic molecules (CRAM). |
format |
Article in Journal/Newspaper |
author |
Helms, John R. Mao, Jingdong Chen, Hongmei Perdue, E. Michael Green, Nelson W. Hatcher, Patrick G. Mopper, Kenneth Stubbins, Aron |
author_facet |
Helms, John R. Mao, Jingdong Chen, Hongmei Perdue, E. Michael Green, Nelson W. Hatcher, Patrick G. Mopper, Kenneth Stubbins, Aron |
author_sort |
Helms, John R. |
title |
Spectroscopic Characterization of Oceanic Dissolved Organic Matter Isolated By Reverse Osmosis Coupled With Electrodialysis |
title_short |
Spectroscopic Characterization of Oceanic Dissolved Organic Matter Isolated By Reverse Osmosis Coupled With Electrodialysis |
title_full |
Spectroscopic Characterization of Oceanic Dissolved Organic Matter Isolated By Reverse Osmosis Coupled With Electrodialysis |
title_fullStr |
Spectroscopic Characterization of Oceanic Dissolved Organic Matter Isolated By Reverse Osmosis Coupled With Electrodialysis |
title_full_unstemmed |
Spectroscopic Characterization of Oceanic Dissolved Organic Matter Isolated By Reverse Osmosis Coupled With Electrodialysis |
title_sort |
spectroscopic characterization of oceanic dissolved organic matter isolated by reverse osmosis coupled with electrodialysis |
publisher |
ODU Digital Commons |
publishDate |
2015 |
url |
https://digitalcommons.odu.edu/chemistry_fac_pubs/148 https://digitalcommons.odu.edu/cgi/viewcontent.cgi?article=1151&context=chemistry_fac_pubs |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic Deep Water North Atlantic |
genre_facet |
North Atlantic Deep Water North Atlantic |
op_source |
Chemistry & Biochemistry Faculty Publications |
op_relation |
https://digitalcommons.odu.edu/chemistry_fac_pubs/148 https://digitalcommons.odu.edu/cgi/viewcontent.cgi?article=1151&context=chemistry_fac_pubs |
_version_ |
1766116865260650496 |