H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes
Oikopleura dioica is a ubiquitous marine zooplankton of biological interest owing to features that include dioecious reproduction, a short life cycle, conserved chordate body plan, and a compact genome. It is an important tunicate model for evolutionary and developmental research, as well as investi...
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ftokinawainstst:oai:oist.repo.nii.ac.jp:00002058 2023-05-15T17:31:20+02:00 H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes Andrew W. Liu Yongkai Tan Aki Masunaga Aleksandra Bliznina Charlotte West Charles Plessy Nicholas M. Luscombe 2021-03-01 http://id.nii.ac.jp/1394/00001851/ https://oist.repo.nii.ac.jp/?action=repository_uri&item_id=2058 https://oist.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2058&item_no=1&attribute_id=22&file_no=1 en eng F1000Research info:pmid/33728042 doi:10.12688/f1000research.25019.2 https://oist.repo.nii.ac.jp/?action=repository_uri&item_id=2058 http://id.nii.ac.jp/1394/00001851/ F1000Research, 9, 780(2021-03-01) 2046-1402 publisher https://oist.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2058&item_no=1&attribute_id=22&file_no=1 © 2021 Liu AW et al. https://doi.org/10.12688/f1000research.25019.2 https://doi.org/10.12688/f1000research.25019.1 karyotype chromosome centromere histone H3 Oikopleura oocyte embryo H3S28P Journal Article 2021 ftokinawainstst https://doi.org/10.12688/f1000research.25019.2 https://doi.org/10.12688/f1000research.25019.1 2022-12-02T00:24:31Z Oikopleura dioica is a ubiquitous marine zooplankton of biological interest owing to features that include dioecious reproduction, a short life cycle, conserved chordate body plan, and a compact genome. It is an important tunicate model for evolutionary and developmental research, as well as investigations into marine ecosystems. The genome of north Atlantic O. dioica comprises three chromosomes. However, comparisons with the genomes of O. dioica sampled from mainland and southern Japan revealed extensive sequence differences. Moreover, historical studies have reported widely varying chromosome counts. We recently initiated a project to study the genomes of O. dioica individuals collected from the coastline of the Ryukyu (Okinawa) Islands in southern Japan. Given the potentially large extent of genomic diversity, we employed karyological techniques to count individual animals’ chromosomes in situ using centromere-specific antibodies directed against H3S28P, a prophase-metaphase cell cycle-specific marker of histone H3. Epifluorescence and confocal images were obtained of embryos and oocytes stained with two commercial anti-H3S28P antibodies (Abcam ab10543 and Thermo Fisher 07-145). The data lead us to conclude that diploid cells from Okinawan O. dioica contain three pairs of chromosomes, in line with the north Atlantic populations. The finding facilitates the telomere-to-telomere assembly of Okinawan O. dioica genome sequences and gives insight into the genomic diversity of O. dioica from different geographical locations. The data deposited in the EBI BioImage Archive provide representative images of the antibodies’ staining properties for use in epifluorescent and confocal based fluorescent microscopy. Article in Journal/Newspaper North Atlantic OIST Institutional Repository F1000Research 9 780 |
institution |
Open Polar |
collection |
OIST Institutional Repository |
op_collection_id |
ftokinawainstst |
language |
English |
topic |
karyotype chromosome centromere histone H3 Oikopleura oocyte embryo H3S28P |
spellingShingle |
karyotype chromosome centromere histone H3 Oikopleura oocyte embryo H3S28P Andrew W. Liu Yongkai Tan Aki Masunaga Aleksandra Bliznina Charlotte West Charles Plessy Nicholas M. Luscombe H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes |
topic_facet |
karyotype chromosome centromere histone H3 Oikopleura oocyte embryo H3S28P |
description |
Oikopleura dioica is a ubiquitous marine zooplankton of biological interest owing to features that include dioecious reproduction, a short life cycle, conserved chordate body plan, and a compact genome. It is an important tunicate model for evolutionary and developmental research, as well as investigations into marine ecosystems. The genome of north Atlantic O. dioica comprises three chromosomes. However, comparisons with the genomes of O. dioica sampled from mainland and southern Japan revealed extensive sequence differences. Moreover, historical studies have reported widely varying chromosome counts. We recently initiated a project to study the genomes of O. dioica individuals collected from the coastline of the Ryukyu (Okinawa) Islands in southern Japan. Given the potentially large extent of genomic diversity, we employed karyological techniques to count individual animals’ chromosomes in situ using centromere-specific antibodies directed against H3S28P, a prophase-metaphase cell cycle-specific marker of histone H3. Epifluorescence and confocal images were obtained of embryos and oocytes stained with two commercial anti-H3S28P antibodies (Abcam ab10543 and Thermo Fisher 07-145). The data lead us to conclude that diploid cells from Okinawan O. dioica contain three pairs of chromosomes, in line with the north Atlantic populations. The finding facilitates the telomere-to-telomere assembly of Okinawan O. dioica genome sequences and gives insight into the genomic diversity of O. dioica from different geographical locations. The data deposited in the EBI BioImage Archive provide representative images of the antibodies’ staining properties for use in epifluorescent and confocal based fluorescent microscopy. |
format |
Article in Journal/Newspaper |
author |
Andrew W. Liu Yongkai Tan Aki Masunaga Aleksandra Bliznina Charlotte West Charles Plessy Nicholas M. Luscombe |
author_facet |
Andrew W. Liu Yongkai Tan Aki Masunaga Aleksandra Bliznina Charlotte West Charles Plessy Nicholas M. Luscombe |
author_sort |
Andrew W. Liu |
title |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes |
title_short |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes |
title_full |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes |
title_fullStr |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes |
title_full_unstemmed |
H3S28P Antibody Staining of Okinawan Oikopleura dioica Suggests the Presence of Three Chromosomes |
title_sort |
h3s28p antibody staining of okinawan oikopleura dioica suggests the presence of three chromosomes |
publisher |
F1000Research |
publishDate |
2021 |
url |
http://id.nii.ac.jp/1394/00001851/ https://oist.repo.nii.ac.jp/?action=repository_uri&item_id=2058 https://oist.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2058&item_no=1&attribute_id=22&file_no=1 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
https://doi.org/10.12688/f1000research.25019.2 https://doi.org/10.12688/f1000research.25019.1 |
op_relation |
info:pmid/33728042 doi:10.12688/f1000research.25019.2 https://oist.repo.nii.ac.jp/?action=repository_uri&item_id=2058 http://id.nii.ac.jp/1394/00001851/ F1000Research, 9, 780(2021-03-01) 2046-1402 publisher https://oist.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2058&item_no=1&attribute_id=22&file_no=1 |
op_rights |
© 2021 Liu AW et al. |
op_doi |
https://doi.org/10.12688/f1000research.25019.2 https://doi.org/10.12688/f1000research.25019.1 |
container_title |
F1000Research |
container_volume |
9 |
container_start_page |
780 |
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1766128849234427904 |