Dual Gene Repertoires for Larval and Adult Shells Reveal Molecules Essential for Molluscan Shell Formation
Molluscan shells, mainly composed of calcium carbonate, also contain organic components such as proteins and polysaccharides. Shell organic matrices construct frameworks of shell structures and regulate crystallization processes during shell formation. To date, a number of shell matrix proteins (SMP...
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Oxford University Press on behalf of the Society for Molecular Biology and Evolution
2018
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ftokinawainstst:oai:oist.repo.nii.ac.jp:00000965 2023-05-15T15:58:50+02:00 Dual Gene Repertoires for Larval and Adult Shells Reveal Molecules Essential for Molluscan Shell Formation Ran Zhao Takeshi Takeuchi Yi-Jyun Luo Akito Ishikawa Tatsushi Kobayashi Ryo Koyanagi Alejandro Villar-Briones Lixy Yamada Hitoshi Sawada Shunsuke Iwanaga Kiyohito Nagai Noriyuki Satoh Kazuyoshi Endo 2018-08-31 http://id.nii.ac.jp/1394/00000874/ https://oist.repo.nii.ac.jp/?action=repository_uri&item_id=965 https://oist.repo.nii.ac.jp/?action=repository_action_common_download&item_id=965&item_no=1&attribute_id=22&file_no=1 en eng Oxford University Press on behalf of the Society for Molecular Biology and Evolution info:pmid/30169718 doi:10.1093/molbev/msy172 https://oist.repo.nii.ac.jp/?action=repository_uri&item_id=965 http://id.nii.ac.jp/1394/00000874/ Molecular Biology and Evolution, 35(11), 2751-2761(2018-08-31) 0737-4038 1537-1719 publisher https://oist.repo.nii.ac.jp/?action=repository_action_common_download&item_id=965&item_no=1&attribute_id=22&file_no=1 © 2018 The Author(s). http://creativecommons.org/licenses/by-nc/4.0/ https://academic.oup.com/mbe/article/35/11/2751/5088508?searchresult=1 Journal Article 2018 ftokinawainstst https://doi.org/10.1093/molbev/msy172 2022-12-02T00:23:43Z Molluscan shells, mainly composed of calcium carbonate, also contain organic components such as proteins and polysaccharides. Shell organic matrices construct frameworks of shell structures and regulate crystallization processes during shell formation. To date, a number of shell matrix proteins (SMPs) have been identified, and their functions in shell formation have been studied. However, previous studies focused only on SMPs extracted from adult shells, secreted after metamorphosis. Using proteomic analyses combined with genomic and transcriptomic analyses, we have identified 31 SMPs from larval shells of the pearl oyster, Pinctada fucata, and 111 from the Pacific oyster, Crassostrea gigas. Larval SMPs are almost entirely different from those of adults in both species. RNA-seq data also confirm that gene expression profiles for larval and adult shell formation are nearly completely different. Therefore, bivalves have two repertoires of SMP genes to construct larval and adult shells. Despite considerable differences in larval and adult SMPs, some functional domains are shared by both SMP repertoires. Conserved domains include von Willebrand factor type A (VWA), chitin-binding (CB), carbonic anhydrase (CA), and acidic domains. These conserved domains are thought to play crucial roles in shell formation. Furthermore, a comprehensive survey of animal genomes revealed that the CA and VWA-CB domain-containing protein families expanded in molluscs after their separation from other Lophotrochozoan linages such as the Brachiopoda. After gene expansion, some family members were co-opted for molluscan SMPs that may have triggered to develop mineralized shells from ancestral, nonmineralized chitinous exoskeletons. Article in Journal/Newspaper Crassostrea gigas Pacific oyster OIST Institutional Repository Pacific Molecular Biology and Evolution |
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Open Polar |
collection |
OIST Institutional Repository |
op_collection_id |
ftokinawainstst |
language |
English |
description |
Molluscan shells, mainly composed of calcium carbonate, also contain organic components such as proteins and polysaccharides. Shell organic matrices construct frameworks of shell structures and regulate crystallization processes during shell formation. To date, a number of shell matrix proteins (SMPs) have been identified, and their functions in shell formation have been studied. However, previous studies focused only on SMPs extracted from adult shells, secreted after metamorphosis. Using proteomic analyses combined with genomic and transcriptomic analyses, we have identified 31 SMPs from larval shells of the pearl oyster, Pinctada fucata, and 111 from the Pacific oyster, Crassostrea gigas. Larval SMPs are almost entirely different from those of adults in both species. RNA-seq data also confirm that gene expression profiles for larval and adult shell formation are nearly completely different. Therefore, bivalves have two repertoires of SMP genes to construct larval and adult shells. Despite considerable differences in larval and adult SMPs, some functional domains are shared by both SMP repertoires. Conserved domains include von Willebrand factor type A (VWA), chitin-binding (CB), carbonic anhydrase (CA), and acidic domains. These conserved domains are thought to play crucial roles in shell formation. Furthermore, a comprehensive survey of animal genomes revealed that the CA and VWA-CB domain-containing protein families expanded in molluscs after their separation from other Lophotrochozoan linages such as the Brachiopoda. After gene expansion, some family members were co-opted for molluscan SMPs that may have triggered to develop mineralized shells from ancestral, nonmineralized chitinous exoskeletons. |
format |
Article in Journal/Newspaper |
author |
Ran Zhao Takeshi Takeuchi Yi-Jyun Luo Akito Ishikawa Tatsushi Kobayashi Ryo Koyanagi Alejandro Villar-Briones Lixy Yamada Hitoshi Sawada Shunsuke Iwanaga Kiyohito Nagai Noriyuki Satoh Kazuyoshi Endo |
spellingShingle |
Ran Zhao Takeshi Takeuchi Yi-Jyun Luo Akito Ishikawa Tatsushi Kobayashi Ryo Koyanagi Alejandro Villar-Briones Lixy Yamada Hitoshi Sawada Shunsuke Iwanaga Kiyohito Nagai Noriyuki Satoh Kazuyoshi Endo Dual Gene Repertoires for Larval and Adult Shells Reveal Molecules Essential for Molluscan Shell Formation |
author_facet |
Ran Zhao Takeshi Takeuchi Yi-Jyun Luo Akito Ishikawa Tatsushi Kobayashi Ryo Koyanagi Alejandro Villar-Briones Lixy Yamada Hitoshi Sawada Shunsuke Iwanaga Kiyohito Nagai Noriyuki Satoh Kazuyoshi Endo |
author_sort |
Ran Zhao |
title |
Dual Gene Repertoires for Larval and Adult Shells Reveal Molecules Essential for Molluscan Shell Formation |
title_short |
Dual Gene Repertoires for Larval and Adult Shells Reveal Molecules Essential for Molluscan Shell Formation |
title_full |
Dual Gene Repertoires for Larval and Adult Shells Reveal Molecules Essential for Molluscan Shell Formation |
title_fullStr |
Dual Gene Repertoires for Larval and Adult Shells Reveal Molecules Essential for Molluscan Shell Formation |
title_full_unstemmed |
Dual Gene Repertoires for Larval and Adult Shells Reveal Molecules Essential for Molluscan Shell Formation |
title_sort |
dual gene repertoires for larval and adult shells reveal molecules essential for molluscan shell formation |
publisher |
Oxford University Press on behalf of the Society for Molecular Biology and Evolution |
publishDate |
2018 |
url |
http://id.nii.ac.jp/1394/00000874/ https://oist.repo.nii.ac.jp/?action=repository_uri&item_id=965 https://oist.repo.nii.ac.jp/?action=repository_action_common_download&item_id=965&item_no=1&attribute_id=22&file_no=1 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
http://creativecommons.org/licenses/by-nc/4.0/ https://academic.oup.com/mbe/article/35/11/2751/5088508?searchresult=1 |
op_relation |
info:pmid/30169718 doi:10.1093/molbev/msy172 https://oist.repo.nii.ac.jp/?action=repository_uri&item_id=965 http://id.nii.ac.jp/1394/00000874/ Molecular Biology and Evolution, 35(11), 2751-2761(2018-08-31) 0737-4038 1537-1719 publisher https://oist.repo.nii.ac.jp/?action=repository_action_common_download&item_id=965&item_no=1&attribute_id=22&file_no=1 |
op_rights |
© 2018 The Author(s). |
op_doi |
https://doi.org/10.1093/molbev/msy172 |
container_title |
Molecular Biology and Evolution |
_version_ |
1766394602020929536 |