Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)

Trypsin inhibitor was purified from the hepatopancreas of squid (Todarodes pacificus). The final inhibitor preparation was nearly homogeneous by SDS-PAGE with an estimated molecular weight of approximately 6300. The squid trypsin inhibitor was acid- and heat-stable, and active against trypsins from...

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Main Authors: Kishimura, Hideki, Saeki, Hiroki, Hayashi, Kenji
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2001
Subjects:
Theragra chalcogramma
Online Access:https://oceanrep.geomar.de/id/eprint/53150/
https://oceanrep.geomar.de/id/eprint/53150/1/3928.pdf
https://doi.org/10.1016/S1096-4959%2801%2900415-8
https://doi.org/10.1016/S1096-4959(01)00415-8
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record_format openpolar
spelling ftoceanrep:oai:oceanrep.geomar.de:53150 2023-05-15T18:32:51+02:00 Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus) Kishimura, Hideki Saeki, Hiroki Hayashi, Kenji 2001-08 text https://oceanrep.geomar.de/id/eprint/53150/ https://oceanrep.geomar.de/id/eprint/53150/1/3928.pdf https://doi.org/10.1016/S1096-4959%2801%2900415-8 https://doi.org/10.1016/S1096-4959(01)00415-8 en eng Elsevier https://oceanrep.geomar.de/id/eprint/53150/1/3928.pdf Kishimura, H., Saeki, H. and Hayashi, K. (2001) Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 130 (1). pp. 117-123. DOI 10.1016/S1096-4959(01)00415-8 <https://doi.org/10.1016/S1096-4959%2801%2900415-8>. doi:10.1016/S1096-4959(01)00415-8 info:eu-repo/semantics/restrictedAccess Article PeerReviewed 2001 ftoceanrep https://doi.org/10.1016/S1096-4959%2801%2900415-810.1016/S1096-4959(01)00415-8 2023-04-07T15:57:10Z Trypsin inhibitor was purified from the hepatopancreas of squid (Todarodes pacificus). The final inhibitor preparation was nearly homogeneous by SDS-PAGE with an estimated molecular weight of approximately 6300. The squid trypsin inhibitor was acid- and heat-stable, and active against trypsins from the pyloric ceca of starfish (Asterias amurensis) and saury (Cololabis saira) and porcine pancreatic trypsin. Amino acid composition of the squid trypsin inhibitor was compared with other invertebrate trypsin inhibitors. The squid trypsin inhibitor inhibited the autolysis of walleye pollock (Theragra chalcogramma) myofibrillar proteins. Article in Journal/Newspaper Theragra chalcogramma OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel)
institution Open Polar
collection OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel)
op_collection_id ftoceanrep
language English
description Trypsin inhibitor was purified from the hepatopancreas of squid (Todarodes pacificus). The final inhibitor preparation was nearly homogeneous by SDS-PAGE with an estimated molecular weight of approximately 6300. The squid trypsin inhibitor was acid- and heat-stable, and active against trypsins from the pyloric ceca of starfish (Asterias amurensis) and saury (Cololabis saira) and porcine pancreatic trypsin. Amino acid composition of the squid trypsin inhibitor was compared with other invertebrate trypsin inhibitors. The squid trypsin inhibitor inhibited the autolysis of walleye pollock (Theragra chalcogramma) myofibrillar proteins.
format Article in Journal/Newspaper
author Kishimura, Hideki
Saeki, Hiroki
Hayashi, Kenji
spellingShingle Kishimura, Hideki
Saeki, Hiroki
Hayashi, Kenji
Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)
author_facet Kishimura, Hideki
Saeki, Hiroki
Hayashi, Kenji
author_sort Kishimura, Hideki
title Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)
title_short Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)
title_full Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)
title_fullStr Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)
title_full_unstemmed Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)
title_sort isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (todarodes pacificus)
publisher Elsevier
publishDate 2001
url https://oceanrep.geomar.de/id/eprint/53150/
https://oceanrep.geomar.de/id/eprint/53150/1/3928.pdf
https://doi.org/10.1016/S1096-4959%2801%2900415-8
https://doi.org/10.1016/S1096-4959(01)00415-8
genre Theragra chalcogramma
genre_facet Theragra chalcogramma
op_relation https://oceanrep.geomar.de/id/eprint/53150/1/3928.pdf
Kishimura, H., Saeki, H. and Hayashi, K. (2001) Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 130 (1). pp. 117-123. DOI 10.1016/S1096-4959(01)00415-8 <https://doi.org/10.1016/S1096-4959%2801%2900415-8>.
doi:10.1016/S1096-4959(01)00415-8
op_rights info:eu-repo/semantics/restrictedAccess
op_doi https://doi.org/10.1016/S1096-4959%2801%2900415-810.1016/S1096-4959(01)00415-8
_version_ 1766217041097785344

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