A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD
An optimized method is presented to determine dissolved free (DFCHO) and dissolved combined carbohydrates (DCCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination of a desalination with electro-dialysis (ED) and high-performance anion exchange chroma...
Published in: | Ocean Science |
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Language: | English |
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Copernicus Publications (EGU)
2020
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Online Access: | https://oceanrep.geomar.de/id/eprint/50137/ https://oceanrep.geomar.de/id/eprint/50137/1/os-16-817-2020.pdf https://os.copernicus.org/articles/16/817/2020/os-16-817-2020.html https://doi.org/10.5194/os-16-817-2020 |
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ftoceanrep:oai:oceanrep.geomar.de:50137 2023-05-15T15:06:04+02:00 A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD Zeppenfeld, Sebastian van Pinxteren, Manuela Engel, Anja Herrmann, Hartmut 2020-07-16 text https://oceanrep.geomar.de/id/eprint/50137/ https://oceanrep.geomar.de/id/eprint/50137/1/os-16-817-2020.pdf https://os.copernicus.org/articles/16/817/2020/os-16-817-2020.html https://doi.org/10.5194/os-16-817-2020 en eng Copernicus Publications (EGU) https://oceanrep.geomar.de/id/eprint/50137/1/os-16-817-2020.pdf Zeppenfeld, S., van Pinxteren, M., Engel, A. and Herrmann, H. (2020) A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD. Open Access Ocean Science, 16 . pp. 817-830. DOI 10.5194/os-16-817-2020 <https://doi.org/10.5194/os-16-817-2020>. doi:10.5194/os-16-817-2020 cc_by_4.0 info:eu-repo/semantics/openAccess Article PeerReviewed 2020 ftoceanrep https://doi.org/10.5194/os-16-817-2020 2023-04-07T15:51:06Z An optimized method is presented to determine dissolved free (DFCHO) and dissolved combined carbohydrates (DCCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination of a desalination with electro-dialysis (ED) and high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). Free neutral sugars, such as glucose and galactose, were found with 95 %–98 % recovery rates. Free amino sugars and free uronic acids were strongly depleted during ED at pH=8, but an adjustment of the pH could result in higher recoveries (58 %–59 % for amino sugars at pH=11; 45 %–49 % for uronic acids at pH=1.5). The applicability of this method for the analysis of DCCHO was evaluated with standard solutions and seawater samples compared with another established desalination method using membrane dialysis. DFCHO in field samples from different regions on Earth ranged between 11 and 118 nM and DCCHO between 260 and 1410 nM. This novel method has the potential to contribute to a better understanding of biogeochemical processes in the oceans and sea–air transfer processes of organic matter into the atmosphere in future studies. Article in Journal/Newspaper Arctic ice core OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Arctic Ocean Science 16 4 817 830 |
institution |
Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
An optimized method is presented to determine dissolved free (DFCHO) and dissolved combined carbohydrates (DCCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination of a desalination with electro-dialysis (ED) and high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). Free neutral sugars, such as glucose and galactose, were found with 95 %–98 % recovery rates. Free amino sugars and free uronic acids were strongly depleted during ED at pH=8, but an adjustment of the pH could result in higher recoveries (58 %–59 % for amino sugars at pH=11; 45 %–49 % for uronic acids at pH=1.5). The applicability of this method for the analysis of DCCHO was evaluated with standard solutions and seawater samples compared with another established desalination method using membrane dialysis. DFCHO in field samples from different regions on Earth ranged between 11 and 118 nM and DCCHO between 260 and 1410 nM. This novel method has the potential to contribute to a better understanding of biogeochemical processes in the oceans and sea–air transfer processes of organic matter into the atmosphere in future studies. |
format |
Article in Journal/Newspaper |
author |
Zeppenfeld, Sebastian van Pinxteren, Manuela Engel, Anja Herrmann, Hartmut |
spellingShingle |
Zeppenfeld, Sebastian van Pinxteren, Manuela Engel, Anja Herrmann, Hartmut A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
author_facet |
Zeppenfeld, Sebastian van Pinxteren, Manuela Engel, Anja Herrmann, Hartmut |
author_sort |
Zeppenfeld, Sebastian |
title |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_short |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_full |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_fullStr |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_full_unstemmed |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_sort |
protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ed) – combined with hpaec-pad |
publisher |
Copernicus Publications (EGU) |
publishDate |
2020 |
url |
https://oceanrep.geomar.de/id/eprint/50137/ https://oceanrep.geomar.de/id/eprint/50137/1/os-16-817-2020.pdf https://os.copernicus.org/articles/16/817/2020/os-16-817-2020.html https://doi.org/10.5194/os-16-817-2020 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic ice core |
genre_facet |
Arctic ice core |
op_relation |
https://oceanrep.geomar.de/id/eprint/50137/1/os-16-817-2020.pdf Zeppenfeld, S., van Pinxteren, M., Engel, A. and Herrmann, H. (2020) A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD. Open Access Ocean Science, 16 . pp. 817-830. DOI 10.5194/os-16-817-2020 <https://doi.org/10.5194/os-16-817-2020>. doi:10.5194/os-16-817-2020 |
op_rights |
cc_by_4.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5194/os-16-817-2020 |
container_title |
Ocean Science |
container_volume |
16 |
container_issue |
4 |
container_start_page |
817 |
op_container_end_page |
830 |
_version_ |
1766337729259372544 |