Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom
Heme b is an iron-containing co-factor in hemoproteins. Heme b concentrations are low (<1 pmol L-1) in iron limited phytoplankton in cultures and in the field. Here, we determined heme b in marine particulate material (>0.7 μm) from the North Atlantic Ocean (GEOVIDE cruise – GEOTRACES section...
Published in: | Frontiers in Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://oceanrep.geomar.de/id/eprint/47195/ https://oceanrep.geomar.de/id/eprint/47195/1/fmicb-10-01566.pdf https://oceanrep.geomar.de/id/eprint/47195/2/Data_Sheet_1_Regulation%20of%20the%20Phytoplankton%20Heme%20b%20Iron%20Pool%20During%20the%20North%20Atlantic%20Spring%20Bloom.zip https://doi.org/10.3389/fmicb.2019.01566 |
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ftoceanrep:oai:oceanrep.geomar.de:47195 2023-05-15T16:51:48+02:00 Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom Louropoulou, Evangelia Gledhill, Martha Browning, Thomas J. Desai, Dhwani K. Menzel Barraqueta, Jan-Lukas Tonnard, Manon Sarthou, Géraldine Planquette, Hélène Bowie, Andrew R. Schmitz, Ruth A. LaRoche, Julie Achterberg, Eric P. 2019-07-11 text archive https://oceanrep.geomar.de/id/eprint/47195/ https://oceanrep.geomar.de/id/eprint/47195/1/fmicb-10-01566.pdf https://oceanrep.geomar.de/id/eprint/47195/2/Data_Sheet_1_Regulation%20of%20the%20Phytoplankton%20Heme%20b%20Iron%20Pool%20During%20the%20North%20Atlantic%20Spring%20Bloom.zip https://doi.org/10.3389/fmicb.2019.01566 en eng Frontiers https://oceanrep.geomar.de/id/eprint/47195/1/fmicb-10-01566.pdf https://oceanrep.geomar.de/id/eprint/47195/2/Data_Sheet_1_Regulation%20of%20the%20Phytoplankton%20Heme%20b%20Iron%20Pool%20During%20the%20North%20Atlantic%20Spring%20Bloom.zip Louropoulou, E., Gledhill, M. , Browning, T. J. , Desai, D. K., Menzel Barraqueta, J. L. , Tonnard, M., Sarthou, G., Planquette, H., Bowie, A. R., Schmitz, R. A., LaRoche, J. and Achterberg, E. P. (2019) Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom. Open Access Frontiers in Microbiology, 10 (1566). DOI 10.3389/fmicb.2019.01566 <https://doi.org/10.3389/fmicb.2019.01566>. doi:10.3389/fmicb.2019.01566 cc_by_4.0 info:eu-repo/semantics/openAccess Article PeerReviewed 2019 ftoceanrep https://doi.org/10.3389/fmicb.2019.01566 2023-04-07T15:46:36Z Heme b is an iron-containing co-factor in hemoproteins. Heme b concentrations are low (<1 pmol L-1) in iron limited phytoplankton in cultures and in the field. Here, we determined heme b in marine particulate material (>0.7 μm) from the North Atlantic Ocean (GEOVIDE cruise – GEOTRACES section GA01), which spanned several biogeochemical regimes. We examined the relationship between heme b abundance and the microbial community composition, and its utility for mapping iron limited phytoplankton. Heme b concentrations ranged from 0.16 to 5.1 pmol L-1 (median = 2.0 pmol L-1, n = 62) in the surface mixed layer (SML) along the cruise track, driven mainly by variability in biomass. However, in the Irminger Basin, the lowest heme b levels (SML: median = 0.53 pmol L-1, n = 12) were observed, whilst the biomass was highest (particulate organic carbon, median = 14.2 μmol L-1, n = 25; chlorophyll a: median = 2.0 nmol L-1, n = 23) pointing to regulatory mechanisms of the heme b pool for growth conservation. Dissolved iron (DFe) was not depleted (SML: median = 0.38 nmol L-1, n = 11) in the Irminger Basin, but large diatoms (Rhizosolenia sp.) dominated. Hence, heme b depletion and regulation is likely to occur during bloom progression when phytoplankton class-dependent absolute iron requirements exceed the available ambient concentration of DFe. Furthermore, high heme b concentrations found in the Iceland Basin and Labrador Sea (median = 3.4 pmol L-1, n = 20), despite having similar DFe concentrations to the Irminger Basin, were attributed to an earlier growth phase of the extant phytoplankton populations. Thus, heme b provides a snapshot of the cellular activity in situ and could both be used as indicator of iron limitation and contribute to understanding phytoplankton adaptation mechanisms to changing iron supplies. Article in Journal/Newspaper Iceland Labrador Sea North Atlantic OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Irminger Basin ENVELOPE(-36.000,-36.000,61.000,61.000) Frontiers in Microbiology 10 |
institution |
Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
Heme b is an iron-containing co-factor in hemoproteins. Heme b concentrations are low (<1 pmol L-1) in iron limited phytoplankton in cultures and in the field. Here, we determined heme b in marine particulate material (>0.7 μm) from the North Atlantic Ocean (GEOVIDE cruise – GEOTRACES section GA01), which spanned several biogeochemical regimes. We examined the relationship between heme b abundance and the microbial community composition, and its utility for mapping iron limited phytoplankton. Heme b concentrations ranged from 0.16 to 5.1 pmol L-1 (median = 2.0 pmol L-1, n = 62) in the surface mixed layer (SML) along the cruise track, driven mainly by variability in biomass. However, in the Irminger Basin, the lowest heme b levels (SML: median = 0.53 pmol L-1, n = 12) were observed, whilst the biomass was highest (particulate organic carbon, median = 14.2 μmol L-1, n = 25; chlorophyll a: median = 2.0 nmol L-1, n = 23) pointing to regulatory mechanisms of the heme b pool for growth conservation. Dissolved iron (DFe) was not depleted (SML: median = 0.38 nmol L-1, n = 11) in the Irminger Basin, but large diatoms (Rhizosolenia sp.) dominated. Hence, heme b depletion and regulation is likely to occur during bloom progression when phytoplankton class-dependent absolute iron requirements exceed the available ambient concentration of DFe. Furthermore, high heme b concentrations found in the Iceland Basin and Labrador Sea (median = 3.4 pmol L-1, n = 20), despite having similar DFe concentrations to the Irminger Basin, were attributed to an earlier growth phase of the extant phytoplankton populations. Thus, heme b provides a snapshot of the cellular activity in situ and could both be used as indicator of iron limitation and contribute to understanding phytoplankton adaptation mechanisms to changing iron supplies. |
format |
Article in Journal/Newspaper |
author |
Louropoulou, Evangelia Gledhill, Martha Browning, Thomas J. Desai, Dhwani K. Menzel Barraqueta, Jan-Lukas Tonnard, Manon Sarthou, Géraldine Planquette, Hélène Bowie, Andrew R. Schmitz, Ruth A. LaRoche, Julie Achterberg, Eric P. |
spellingShingle |
Louropoulou, Evangelia Gledhill, Martha Browning, Thomas J. Desai, Dhwani K. Menzel Barraqueta, Jan-Lukas Tonnard, Manon Sarthou, Géraldine Planquette, Hélène Bowie, Andrew R. Schmitz, Ruth A. LaRoche, Julie Achterberg, Eric P. Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom |
author_facet |
Louropoulou, Evangelia Gledhill, Martha Browning, Thomas J. Desai, Dhwani K. Menzel Barraqueta, Jan-Lukas Tonnard, Manon Sarthou, Géraldine Planquette, Hélène Bowie, Andrew R. Schmitz, Ruth A. LaRoche, Julie Achterberg, Eric P. |
author_sort |
Louropoulou, Evangelia |
title |
Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom |
title_short |
Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom |
title_full |
Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom |
title_fullStr |
Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom |
title_full_unstemmed |
Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom |
title_sort |
regulation of the phytoplankton heme b iron pool during the north atlantic spring bloom |
publisher |
Frontiers |
publishDate |
2019 |
url |
https://oceanrep.geomar.de/id/eprint/47195/ https://oceanrep.geomar.de/id/eprint/47195/1/fmicb-10-01566.pdf https://oceanrep.geomar.de/id/eprint/47195/2/Data_Sheet_1_Regulation%20of%20the%20Phytoplankton%20Heme%20b%20Iron%20Pool%20During%20the%20North%20Atlantic%20Spring%20Bloom.zip https://doi.org/10.3389/fmicb.2019.01566 |
long_lat |
ENVELOPE(-36.000,-36.000,61.000,61.000) |
geographic |
Irminger Basin |
geographic_facet |
Irminger Basin |
genre |
Iceland Labrador Sea North Atlantic |
genre_facet |
Iceland Labrador Sea North Atlantic |
op_relation |
https://oceanrep.geomar.de/id/eprint/47195/1/fmicb-10-01566.pdf https://oceanrep.geomar.de/id/eprint/47195/2/Data_Sheet_1_Regulation%20of%20the%20Phytoplankton%20Heme%20b%20Iron%20Pool%20During%20the%20North%20Atlantic%20Spring%20Bloom.zip Louropoulou, E., Gledhill, M. , Browning, T. J. , Desai, D. K., Menzel Barraqueta, J. L. , Tonnard, M., Sarthou, G., Planquette, H., Bowie, A. R., Schmitz, R. A., LaRoche, J. and Achterberg, E. P. (2019) Regulation of the Phytoplankton Heme b Iron Pool During the North Atlantic Spring Bloom. Open Access Frontiers in Microbiology, 10 (1566). DOI 10.3389/fmicb.2019.01566 <https://doi.org/10.3389/fmicb.2019.01566>. doi:10.3389/fmicb.2019.01566 |
op_rights |
cc_by_4.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmicb.2019.01566 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
_version_ |
1766041888188530688 |