Global distribution of a wild alga revealed by targeted metagenomics
Eukaryotic phytoplankton play key roles in atmospheric CO2 uptake and sequestration in marine environments 1, 2. Community shifts attributed to climate change have already been reported in the Arctic ocean, where tiny, photosynthetic picoeukaryotes (≤3 μm diameter) have increased, while larger taxa...
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ftoceanrep:oai:oceanrep.geomar.de:46035 2023-05-15T15:08:50+02:00 Global distribution of a wild alga revealed by targeted metagenomics Worden, Alexandra Z. Janouskovec, J. McRose, D. Engman, A. Welsh, R. M. Malfatti, S. Tringe, S. G. Keeling, P. J. 2012 text https://oceanrep.geomar.de/id/eprint/46035/ https://oceanrep.geomar.de/id/eprint/46035/1/Global-distribution-of-a-wild-alga-revealed-by-targeted-metagenomicsCurrent-Biology.pdf https://doi.org/10.1016/j.cub.2012.07.054 en eng Elsevier https://oceanrep.geomar.de/id/eprint/46035/1/Global-distribution-of-a-wild-alga-revealed-by-targeted-metagenomicsCurrent-Biology.pdf Worden, A. Z. , Janouskovec, J., McRose, D., Engman, A., Welsh, R. M., Malfatti, S., Tringe, S. G. and Keeling, P. J. (2012) Global distribution of a wild alga revealed by targeted metagenomics. Open Access Current Biology, 22 (17). R675-R677. DOI 10.1016/j.cub.2012.07.054 <https://doi.org/10.1016/j.cub.2012.07.054>. doi:10.1016/j.cub.2012.07.054 cc_by_3.0 info:eu-repo/semantics/openAccess Article PeerReviewed 2012 ftoceanrep https://doi.org/10.1016/j.cub.2012.07.054 2023-04-07T15:44:08Z Eukaryotic phytoplankton play key roles in atmospheric CO2 uptake and sequestration in marine environments 1, 2. Community shifts attributed to climate change have already been reported in the Arctic ocean, where tiny, photosynthetic picoeukaryotes (≤3 μm diameter) have increased, while larger taxa have decreased [3]. Unfortunately, for vast regions of the world's oceans, little is known about distributions of different genera and levels of genetic variation between ocean basins. This lack of baseline information makes it impossible to assess the impacts of environmental change on phytoplankton diversity, and global carbon cycling. A major knowledge impediment is that these organisms are highly diverse, and most remain uncultured [2]. Metagenomics avoids the culturing step and provides insights into genes present in the environment without some of the biases associated with conventional molecular survey methods. However, connecting metagenomic sequences to the organisms containing them is challenging. For many unicellular eukaryotes the reference genomes needed to make this connection are not available. We circumvented this problem using at-sea fluorescence activated cell sorting (FACS) to separate abundant natural populations of photosynthetic eukaryotes and sequence their DNA, generating reference genome information while eliminating the need for culturing [2]. Here, we present the complete chloroplast genome from an Atlantic picoeukaryote population and discoveries it enabled on the evolution, distribution, and potential carbon sequestration role of a tiny, wild alga Article in Journal/Newspaper Arctic Arctic Ocean Climate change Phytoplankton OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Arctic Arctic Ocean Current Biology 22 17 R675 R677 |
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OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
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ftoceanrep |
language |
English |
description |
Eukaryotic phytoplankton play key roles in atmospheric CO2 uptake and sequestration in marine environments 1, 2. Community shifts attributed to climate change have already been reported in the Arctic ocean, where tiny, photosynthetic picoeukaryotes (≤3 μm diameter) have increased, while larger taxa have decreased [3]. Unfortunately, for vast regions of the world's oceans, little is known about distributions of different genera and levels of genetic variation between ocean basins. This lack of baseline information makes it impossible to assess the impacts of environmental change on phytoplankton diversity, and global carbon cycling. A major knowledge impediment is that these organisms are highly diverse, and most remain uncultured [2]. Metagenomics avoids the culturing step and provides insights into genes present in the environment without some of the biases associated with conventional molecular survey methods. However, connecting metagenomic sequences to the organisms containing them is challenging. For many unicellular eukaryotes the reference genomes needed to make this connection are not available. We circumvented this problem using at-sea fluorescence activated cell sorting (FACS) to separate abundant natural populations of photosynthetic eukaryotes and sequence their DNA, generating reference genome information while eliminating the need for culturing [2]. Here, we present the complete chloroplast genome from an Atlantic picoeukaryote population and discoveries it enabled on the evolution, distribution, and potential carbon sequestration role of a tiny, wild alga |
format |
Article in Journal/Newspaper |
author |
Worden, Alexandra Z. Janouskovec, J. McRose, D. Engman, A. Welsh, R. M. Malfatti, S. Tringe, S. G. Keeling, P. J. |
spellingShingle |
Worden, Alexandra Z. Janouskovec, J. McRose, D. Engman, A. Welsh, R. M. Malfatti, S. Tringe, S. G. Keeling, P. J. Global distribution of a wild alga revealed by targeted metagenomics |
author_facet |
Worden, Alexandra Z. Janouskovec, J. McRose, D. Engman, A. Welsh, R. M. Malfatti, S. Tringe, S. G. Keeling, P. J. |
author_sort |
Worden, Alexandra Z. |
title |
Global distribution of a wild alga revealed by targeted metagenomics |
title_short |
Global distribution of a wild alga revealed by targeted metagenomics |
title_full |
Global distribution of a wild alga revealed by targeted metagenomics |
title_fullStr |
Global distribution of a wild alga revealed by targeted metagenomics |
title_full_unstemmed |
Global distribution of a wild alga revealed by targeted metagenomics |
title_sort |
global distribution of a wild alga revealed by targeted metagenomics |
publisher |
Elsevier |
publishDate |
2012 |
url |
https://oceanrep.geomar.de/id/eprint/46035/ https://oceanrep.geomar.de/id/eprint/46035/1/Global-distribution-of-a-wild-alga-revealed-by-targeted-metagenomicsCurrent-Biology.pdf https://doi.org/10.1016/j.cub.2012.07.054 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean Climate change Phytoplankton |
genre_facet |
Arctic Arctic Ocean Climate change Phytoplankton |
op_relation |
https://oceanrep.geomar.de/id/eprint/46035/1/Global-distribution-of-a-wild-alga-revealed-by-targeted-metagenomicsCurrent-Biology.pdf Worden, A. Z. , Janouskovec, J., McRose, D., Engman, A., Welsh, R. M., Malfatti, S., Tringe, S. G. and Keeling, P. J. (2012) Global distribution of a wild alga revealed by targeted metagenomics. Open Access Current Biology, 22 (17). R675-R677. DOI 10.1016/j.cub.2012.07.054 <https://doi.org/10.1016/j.cub.2012.07.054>. doi:10.1016/j.cub.2012.07.054 |
op_rights |
cc_by_3.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.cub.2012.07.054 |
container_title |
Current Biology |
container_volume |
22 |
container_issue |
17 |
container_start_page |
R675 |
op_container_end_page |
R677 |
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1766340120180424704 |