Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations
Spliceosomal introns are a hallmark of eukaryotic genes that are hypothesized to play important roles in genome evolution but have poorly understood origins. Although most introns lack sequence homology to each other, new families of spliceosomal introns that are repeated hundreds of times in indivi...
Published in: | Molecular Biology and Evolution |
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Oxford University Press
2015
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Online Access: | https://oceanrep.geomar.de/id/eprint/46023/ https://oceanrep.geomar.de/id/eprint/46023/1/Intron-invasions-trace-algal-speciation-and-reveal-nearly-identical-arctic-and-antarctic-micromonas-populationsMolecular-Biology-and-Evolution.pdf https://doi.org/10.1093/molbev/msv122 |
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ftoceanrep:oai:oceanrep.geomar.de:46023 2023-05-15T13:58:15+02:00 Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations Simmons, Melinda P. Bachy, Charles Sudek, Sebastian Van Baren, Marijke J. Sudek, Lisa Ares, Manuel Worden, Alexandra Z. 2015 text https://oceanrep.geomar.de/id/eprint/46023/ https://oceanrep.geomar.de/id/eprint/46023/1/Intron-invasions-trace-algal-speciation-and-reveal-nearly-identical-arctic-and-antarctic-micromonas-populationsMolecular-Biology-and-Evolution.pdf https://doi.org/10.1093/molbev/msv122 en eng Oxford University Press https://oceanrep.geomar.de/id/eprint/46023/1/Intron-invasions-trace-algal-speciation-and-reveal-nearly-identical-arctic-and-antarctic-micromonas-populationsMolecular-Biology-and-Evolution.pdf Simmons, M. P., Bachy, C., Sudek, S., Van Baren, M. J., Sudek, L., Ares, M. and Worden, A. Z. (2015) Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations. Open Access Molecular Biology and Evolution, 32 (9). pp. 2219-2235. DOI 10.1093/molbev/msv122 <https://doi.org/10.1093/molbev%2Fmsv122>. doi:10.1093/molbev/msv122 cc_by_4.0 info:eu-repo/semantics/openAccess Article PeerReviewed 2015 ftoceanrep https://doi.org/10.1093/molbev/msv122 2023-04-07T15:44:08Z Spliceosomal introns are a hallmark of eukaryotic genes that are hypothesized to play important roles in genome evolution but have poorly understood origins. Although most introns lack sequence homology to each other, new families of spliceosomal introns that are repeated hundreds of times in individual genomes have recently been discovered in a few organisms. The prevalence and conservation of these introner elements (IEs) or introner-like elements in other taxa, as well as their evolutionary relationships to regular spliceosomal introns, are still unknown. Here, we systematically investigate introns in the widespread marine green alga Micromonas and report new families of IEs, numerous intron presence-absence polymorphisms, and potential intron insertion hot-spots. The new families enabled identification of conserved IE secondary structure features and establishment of a novel general model for repetitive intron proliferation across genomes. Despite shared secondary structure, the IE families from each Micromonas lineage bear no obvious sequence similarity to those in the other lineages, suggesting that their appearance is intimately linked with the process of speciation. Two of the new IE families come from an Arctic culture (Micromonas Clade E2) isolated from a polar region where abundance of this alga is increasing due to climate induced changes. The same two families were detected in metagenomic data from Antarctica-a system where Micromonas has never before been reported. Strikingly high identity between the Arctic isolate and Antarctic coding sequences that flank the IEs suggests connectivity between populations in the two polar systems that we postulate occurs through deep-sea currents. Recovery of Clade E2 sequences in North Atlantic Deep Waters beneath the Gulf Stream supports this hypothesis. Our research illuminates the dynamic relationships between an unusual class of repetitive introns, genome evolution, speciation, and global distribution of this sentinel marine alga. © 2015 The Author. Article in Journal/Newspaper Antarc* Antarctic Antarctica Arctic Arctic North Atlantic OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Antarctic Arctic Molecular Biology and Evolution 32 9 2219 2235 |
institution |
Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
Spliceosomal introns are a hallmark of eukaryotic genes that are hypothesized to play important roles in genome evolution but have poorly understood origins. Although most introns lack sequence homology to each other, new families of spliceosomal introns that are repeated hundreds of times in individual genomes have recently been discovered in a few organisms. The prevalence and conservation of these introner elements (IEs) or introner-like elements in other taxa, as well as their evolutionary relationships to regular spliceosomal introns, are still unknown. Here, we systematically investigate introns in the widespread marine green alga Micromonas and report new families of IEs, numerous intron presence-absence polymorphisms, and potential intron insertion hot-spots. The new families enabled identification of conserved IE secondary structure features and establishment of a novel general model for repetitive intron proliferation across genomes. Despite shared secondary structure, the IE families from each Micromonas lineage bear no obvious sequence similarity to those in the other lineages, suggesting that their appearance is intimately linked with the process of speciation. Two of the new IE families come from an Arctic culture (Micromonas Clade E2) isolated from a polar region where abundance of this alga is increasing due to climate induced changes. The same two families were detected in metagenomic data from Antarctica-a system where Micromonas has never before been reported. Strikingly high identity between the Arctic isolate and Antarctic coding sequences that flank the IEs suggests connectivity between populations in the two polar systems that we postulate occurs through deep-sea currents. Recovery of Clade E2 sequences in North Atlantic Deep Waters beneath the Gulf Stream supports this hypothesis. Our research illuminates the dynamic relationships between an unusual class of repetitive introns, genome evolution, speciation, and global distribution of this sentinel marine alga. © 2015 The Author. |
format |
Article in Journal/Newspaper |
author |
Simmons, Melinda P. Bachy, Charles Sudek, Sebastian Van Baren, Marijke J. Sudek, Lisa Ares, Manuel Worden, Alexandra Z. |
spellingShingle |
Simmons, Melinda P. Bachy, Charles Sudek, Sebastian Van Baren, Marijke J. Sudek, Lisa Ares, Manuel Worden, Alexandra Z. Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations |
author_facet |
Simmons, Melinda P. Bachy, Charles Sudek, Sebastian Van Baren, Marijke J. Sudek, Lisa Ares, Manuel Worden, Alexandra Z. |
author_sort |
Simmons, Melinda P. |
title |
Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations |
title_short |
Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations |
title_full |
Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations |
title_fullStr |
Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations |
title_full_unstemmed |
Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations |
title_sort |
intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations |
publisher |
Oxford University Press |
publishDate |
2015 |
url |
https://oceanrep.geomar.de/id/eprint/46023/ https://oceanrep.geomar.de/id/eprint/46023/1/Intron-invasions-trace-algal-speciation-and-reveal-nearly-identical-arctic-and-antarctic-micromonas-populationsMolecular-Biology-and-Evolution.pdf https://doi.org/10.1093/molbev/msv122 |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Antarctica Arctic Arctic North Atlantic |
genre_facet |
Antarc* Antarctic Antarctica Arctic Arctic North Atlantic |
op_relation |
https://oceanrep.geomar.de/id/eprint/46023/1/Intron-invasions-trace-algal-speciation-and-reveal-nearly-identical-arctic-and-antarctic-micromonas-populationsMolecular-Biology-and-Evolution.pdf Simmons, M. P., Bachy, C., Sudek, S., Van Baren, M. J., Sudek, L., Ares, M. and Worden, A. Z. (2015) Intron invasions trace algal speciation and reveal nearly identical arctic and antarctic micromonas populations. Open Access Molecular Biology and Evolution, 32 (9). pp. 2219-2235. DOI 10.1093/molbev/msv122 <https://doi.org/10.1093/molbev%2Fmsv122>. doi:10.1093/molbev/msv122 |
op_rights |
cc_by_4.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1093/molbev/msv122 |
container_title |
Molecular Biology and Evolution |
container_volume |
32 |
container_issue |
9 |
container_start_page |
2219 |
op_container_end_page |
2235 |
_version_ |
1766266436452352000 |