Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic
The enzymatic electron transport system (ETS) assay is frequently used as a proxy of respiratory activity in planktonic communities. It is thought to estimate the maximum overall activity of the enzymes associated with the respiratory ETS systems in both eukaryotic and prokaryotic organisms. Thus, i...
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Online Access: | https://oceanrep.geomar.de/id/eprint/44366/ https://oceanrep.geomar.de/id/eprint/44366/1/fmars-05-00310.pdf https://doi.org/10.3389/fmars.2018.00310 |
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ftoceanrep:oai:oceanrep.geomar.de:44366 2023-05-15T17:36:15+02:00 Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic Filella, Alba Baños, Isabel Montero, María F. Hernández-Hernández, Nauzet Rodríguez-Santos, Adriana Ludwig, Andrea Riebesell, Ulf Arístegui, Javier 2018-08-28 text https://oceanrep.geomar.de/id/eprint/44366/ https://oceanrep.geomar.de/id/eprint/44366/1/fmars-05-00310.pdf https://doi.org/10.3389/fmars.2018.00310 en eng Frontiers https://oceanrep.geomar.de/id/eprint/44366/1/fmars-05-00310.pdf Filella, A., Baños, I., Montero, M. F., Hernández-Hernández, N., Rodríguez-Santos, A., Ludwig, A., Riebesell, U. and Arístegui, J. (2018) Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic. Open Access Frontiers in Marine Science, 5 . Art.Nr. 310. DOI 10.3389/fmars.2018.00310 <https://doi.org/10.3389/fmars.2018.00310>. doi:10.3389/fmars.2018.00310 cc_by_4.0 info:eu-repo/semantics/openAccess Article PeerReviewed 2018 ftoceanrep https://doi.org/10.3389/fmars.2018.00310 2023-04-07T15:41:22Z The enzymatic electron transport system (ETS) assay is frequently used as a proxy of respiratory activity in planktonic communities. It is thought to estimate the maximum overall activity of the enzymes associated with the respiratory ETS systems in both eukaryotic and prokaryotic organisms. Thus, in order to derive actual respiration rates (R) from ETS it is necessary to determine empirical R/ETS conversion algorithms. In this study we explore the temporal development of R and ETS activity in natural plankton communities (from bacteria to large phytoplankton) enclosed in mesocosms, treated with different CO2 concentrations. The experiment lasted 30 days, during which abrupt changes in community structure and biomass occurred through a sharp transition from oligotrophy (phase I) to highly eutrophic conditions (phase II) after nutrient-induced fertilization (day 18). R and ETS did not show any response to CO2 under oligotrophic conditions, but R increased significantly more in the two high CO2 mesocosms after fertilization, coinciding with a sharp rise in large phytoplankton (mostly diatoms). R and ETS were significantly correlated only during the eutrophic phase. The R/ETS ranged more than threefold in magnitude during the experiment, with phase-averaged values significantly higher under oligotrophic conditions (0.7-1.1) than after nutrient fertilization (0.5-0.7). We did not find any significant relationship between R/ETS and community structure or biomass, although R correlated significantly with total biomass after fertilization in the four mesocosms. Multiple stepwise regression models show that large phytoplankton explains most of the variance in R during phases I (86%) and II (53%) and of ETS (86%) during phase II, while picophytoplankton contributes up to 73% to explain the variance in the ETS model during phase I. Our results suggest that R/ETS may be too variable in the ocean as to apply constant values to different communities living under contrasting environmental conditions. Controlled experiments ... Article in Journal/Newspaper North Atlantic OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Frontiers in Marine Science 5 |
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Open Polar |
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OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
The enzymatic electron transport system (ETS) assay is frequently used as a proxy of respiratory activity in planktonic communities. It is thought to estimate the maximum overall activity of the enzymes associated with the respiratory ETS systems in both eukaryotic and prokaryotic organisms. Thus, in order to derive actual respiration rates (R) from ETS it is necessary to determine empirical R/ETS conversion algorithms. In this study we explore the temporal development of R and ETS activity in natural plankton communities (from bacteria to large phytoplankton) enclosed in mesocosms, treated with different CO2 concentrations. The experiment lasted 30 days, during which abrupt changes in community structure and biomass occurred through a sharp transition from oligotrophy (phase I) to highly eutrophic conditions (phase II) after nutrient-induced fertilization (day 18). R and ETS did not show any response to CO2 under oligotrophic conditions, but R increased significantly more in the two high CO2 mesocosms after fertilization, coinciding with a sharp rise in large phytoplankton (mostly diatoms). R and ETS were significantly correlated only during the eutrophic phase. The R/ETS ranged more than threefold in magnitude during the experiment, with phase-averaged values significantly higher under oligotrophic conditions (0.7-1.1) than after nutrient fertilization (0.5-0.7). We did not find any significant relationship between R/ETS and community structure or biomass, although R correlated significantly with total biomass after fertilization in the four mesocosms. Multiple stepwise regression models show that large phytoplankton explains most of the variance in R during phases I (86%) and II (53%) and of ETS (86%) during phase II, while picophytoplankton contributes up to 73% to explain the variance in the ETS model during phase I. Our results suggest that R/ETS may be too variable in the ocean as to apply constant values to different communities living under contrasting environmental conditions. Controlled experiments ... |
format |
Article in Journal/Newspaper |
author |
Filella, Alba Baños, Isabel Montero, María F. Hernández-Hernández, Nauzet Rodríguez-Santos, Adriana Ludwig, Andrea Riebesell, Ulf Arístegui, Javier |
spellingShingle |
Filella, Alba Baños, Isabel Montero, María F. Hernández-Hernández, Nauzet Rodríguez-Santos, Adriana Ludwig, Andrea Riebesell, Ulf Arístegui, Javier Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
author_facet |
Filella, Alba Baños, Isabel Montero, María F. Hernández-Hernández, Nauzet Rodríguez-Santos, Adriana Ludwig, Andrea Riebesell, Ulf Arístegui, Javier |
author_sort |
Filella, Alba |
title |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_short |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_full |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_fullStr |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_full_unstemmed |
Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic |
title_sort |
plankton community respiration and ets activity under variable co2 and nutrient fertilization during a mesocosm study in the subtropical north atlantic |
publisher |
Frontiers |
publishDate |
2018 |
url |
https://oceanrep.geomar.de/id/eprint/44366/ https://oceanrep.geomar.de/id/eprint/44366/1/fmars-05-00310.pdf https://doi.org/10.3389/fmars.2018.00310 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
https://oceanrep.geomar.de/id/eprint/44366/1/fmars-05-00310.pdf Filella, A., Baños, I., Montero, M. F., Hernández-Hernández, N., Rodríguez-Santos, A., Ludwig, A., Riebesell, U. and Arístegui, J. (2018) Plankton Community Respiration and ETS Activity Under Variable CO2 and Nutrient Fertilization During a Mesocosm Study in the Subtropical North Atlantic. Open Access Frontiers in Marine Science, 5 . Art.Nr. 310. DOI 10.3389/fmars.2018.00310 <https://doi.org/10.3389/fmars.2018.00310>. doi:10.3389/fmars.2018.00310 |
op_rights |
cc_by_4.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmars.2018.00310 |
container_title |
Frontiers in Marine Science |
container_volume |
5 |
_version_ |
1766135670731964416 |