Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level
European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offsp...
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Public Library of Science
2018
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Online Access: | https://oceanrep.geomar.de/id/eprint/43626/ https://oceanrep.geomar.de/id/eprint/43626/1/journal.pone.0198294-1.pdf https://oceanrep.geomar.de/id/eprint/43626/6/pone.0198294.s001.xlsx https://doi.org/10.1371/journal.pone.0198294 |
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ftoceanrep:oai:oceanrep.geomar.de:43626 2023-05-15T13:28:15+02:00 Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Tomkiewicz, Jonna Butts, Ian A. E. 2018-06-13 text other https://oceanrep.geomar.de/id/eprint/43626/ https://oceanrep.geomar.de/id/eprint/43626/1/journal.pone.0198294-1.pdf https://oceanrep.geomar.de/id/eprint/43626/6/pone.0198294.s001.xlsx https://doi.org/10.1371/journal.pone.0198294 en eng Public Library of Science https://oceanrep.geomar.de/id/eprint/43626/1/journal.pone.0198294-1.pdf https://oceanrep.geomar.de/id/eprint/43626/6/pone.0198294.s001.xlsx Politis, S. N., Mazurais, D., Servili, A., Zambonino-Infante, J. L., Miest, J. J., Tomkiewicz, J. and Butts, I. A. E. (2018) Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level. Open Access PLoS ONE, 13 (6). e0198294. DOI 10.1371/journal.pone.0198294 <https://doi.org/10.1371/journal.pone.0198294>. doi:10.1371/journal.pone.0198294 cc_by_4.0 info:eu-repo/semantics/openAccess Article PeerReviewed 2018 ftoceanrep https://doi.org/10.1371/journal.pone.0198294 2023-04-07T15:40:31Z European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions. Results showed that several genes relating to osmoregulation (nkcc2α, nkcc2β, aqp1dup, aqpe), stress response (hsp70, hsp90), and thyroid metabolism (thrαA, thrαB, thrβB, dio1, dio2, dio3) were differentially expressed throughout larval development, while nkcc1α, nkcc2β, aqp3, aqp1dup, aqpe, hsp90, thrαA and dio3 showed lower expression in response to the salinity reduction. Moreover, larvae were able to keep energy metabolism related gene expression (atp6, cox1) at stable levels, irrespective of the salinity reduction. As such, when reducing salinity, an energy surplus associated to reduced osmoregulation demands and stress (lower nkcc, aqp and hsp expression), likely facilitated the observed increased survival, improved biometry and enhanced growth efficiency. Additionally, the salinity reduction decreased the amount of severe deformities such as spinal curvature and emaciation but also induced an edematous state of the larval heart, resulting in the most balanced mortality/deformity ratio when salinity was decreased on 3 dph and at 2 psu/day. However, the persistency of the pericardial edema and if or how it represents an obstacle in further larval development needs to be further clarified. In conclusion, this study clearly showed that salinity reduction regimes towards iso-osmotic conditions facilitated the European eel pre-leptocephalus development and revealed the existence of highly sensitive and regulated osmoregulation processes at such early life stage of this species. Article in Journal/Newspaper Anguilla anguilla European eel OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) PLOS ONE 13 6 e0198294 |
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Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions. Results showed that several genes relating to osmoregulation (nkcc2α, nkcc2β, aqp1dup, aqpe), stress response (hsp70, hsp90), and thyroid metabolism (thrαA, thrαB, thrβB, dio1, dio2, dio3) were differentially expressed throughout larval development, while nkcc1α, nkcc2β, aqp3, aqp1dup, aqpe, hsp90, thrαA and dio3 showed lower expression in response to the salinity reduction. Moreover, larvae were able to keep energy metabolism related gene expression (atp6, cox1) at stable levels, irrespective of the salinity reduction. As such, when reducing salinity, an energy surplus associated to reduced osmoregulation demands and stress (lower nkcc, aqp and hsp expression), likely facilitated the observed increased survival, improved biometry and enhanced growth efficiency. Additionally, the salinity reduction decreased the amount of severe deformities such as spinal curvature and emaciation but also induced an edematous state of the larval heart, resulting in the most balanced mortality/deformity ratio when salinity was decreased on 3 dph and at 2 psu/day. However, the persistency of the pericardial edema and if or how it represents an obstacle in further larval development needs to be further clarified. In conclusion, this study clearly showed that salinity reduction regimes towards iso-osmotic conditions facilitated the European eel pre-leptocephalus development and revealed the existence of highly sensitive and regulated osmoregulation processes at such early life stage of this species. |
format |
Article in Journal/Newspaper |
author |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Tomkiewicz, Jonna Butts, Ian A. E. |
spellingShingle |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Tomkiewicz, Jonna Butts, Ian A. E. Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
author_facet |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Tomkiewicz, Jonna Butts, Ian A. E. |
author_sort |
Politis, Sebastian N. |
title |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_short |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_full |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_fullStr |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_full_unstemmed |
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level |
title_sort |
salinity reduction benefits european eel larvae: insights at the morphological and molecular level |
publisher |
Public Library of Science |
publishDate |
2018 |
url |
https://oceanrep.geomar.de/id/eprint/43626/ https://oceanrep.geomar.de/id/eprint/43626/1/journal.pone.0198294-1.pdf https://oceanrep.geomar.de/id/eprint/43626/6/pone.0198294.s001.xlsx https://doi.org/10.1371/journal.pone.0198294 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
https://oceanrep.geomar.de/id/eprint/43626/1/journal.pone.0198294-1.pdf https://oceanrep.geomar.de/id/eprint/43626/6/pone.0198294.s001.xlsx Politis, S. N., Mazurais, D., Servili, A., Zambonino-Infante, J. L., Miest, J. J., Tomkiewicz, J. and Butts, I. A. E. (2018) Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level. Open Access PLoS ONE, 13 (6). e0198294. DOI 10.1371/journal.pone.0198294 <https://doi.org/10.1371/journal.pone.0198294>. doi:10.1371/journal.pone.0198294 |
op_rights |
cc_by_4.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0198294 |
container_title |
PLOS ONE |
container_volume |
13 |
container_issue |
6 |
container_start_page |
e0198294 |
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1766403074000158720 |