In vivo characterization of bivalve larval shells: a confocal Raman microscopy study
In vivo confocal Raman microscopy (CRM), polarized light microscopy and Fourier transform infrared spectroscopy (FTIR) were used to determine if a significant amount of amorphous calcium carbonate (ACC) exists within larval shells of Baltic mytilid mussels (Mytilus edulis-like) and whether the amoun...
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ftoceanrep:oai:oceanrep.geomar.de:42882 2023-05-15T15:58:41+02:00 In vivo characterization of bivalve larval shells: a confocal Raman microscopy study Ramesh, Kirti Melzner, Frank Griffith, Andrew W. Gobler, Christopher J. Rouger, Caroline Tasdemir, Deniz Nehrke, Gernot 2018 text https://oceanrep.geomar.de/id/eprint/42882/ https://oceanrep.geomar.de/id/eprint/42882/1/Ramesh%20et%20al%202018.pdf https://doi.org/10.1098/rsif.2017.0723 en eng The Royal Society https://oceanrep.geomar.de/id/eprint/42882/1/Ramesh%20et%20al%202018.pdf Ramesh, K. , Melzner, F., Griffith, A. W., Gobler, C. J., Rouger, C., Tasdemir, D. and Nehrke, G. (2018) In vivo characterization of bivalve larval shells: a confocal Raman microscopy study . Journal of The Royal Society Interface, 15 (141). p. 20170723. DOI 10.1098/rsif.2017.0723 <https://doi.org/10.1098/rsif.2017.0723>. doi:10.1098/rsif.2017.0723 info:eu-repo/semantics/restrictedAccess Article PeerReviewed info:eu-repo/semantics/article 2018 ftoceanrep https://doi.org/10.1098/rsif.2017.0723 2023-04-07T15:39:40Z In vivo confocal Raman microscopy (CRM), polarized light microscopy and Fourier transform infrared spectroscopy (FTIR) were used to determine if a significant amount of amorphous calcium carbonate (ACC) exists within larval shells of Baltic mytilid mussels (Mytilus edulis-like) and whether the amount of ACC varies during larval development. No evidence for ACC was found from the onset of shell deposition at 21 h post-fertilization (hpf) until 48 hpf. Larval Mytilus shells were crystalline from 21 hpf onwards and exhibited CRM and FTIR peaks characteristic of aragonite. Prior to shell deposition at 21 hpf, no evidence for carbonates was observed through in vivo CRM.We further analysed the composition of larval shells in three other bivalve species, Mercenaria mercenaria, Crassostrea gigas and Crassostrea virginica and observed no evidence for ACC, which is in contrast to previous work on the same species. Our findings indicate that larval bivalve shells are composed of crystalline aragonite and we demonstrate that conflicting results are related to sub-optimal measurements and misinterpretation of CRM spectra. Our results demonstrate that the common perception that ACC generally occurs as a stable and abundant precursor during larval bivalve calcification needs to be critically reviewed. Article in Journal/Newspaper Crassostrea gigas OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Journal of The Royal Society Interface 15 141 20170723 |
institution |
Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
In vivo confocal Raman microscopy (CRM), polarized light microscopy and Fourier transform infrared spectroscopy (FTIR) were used to determine if a significant amount of amorphous calcium carbonate (ACC) exists within larval shells of Baltic mytilid mussels (Mytilus edulis-like) and whether the amount of ACC varies during larval development. No evidence for ACC was found from the onset of shell deposition at 21 h post-fertilization (hpf) until 48 hpf. Larval Mytilus shells were crystalline from 21 hpf onwards and exhibited CRM and FTIR peaks characteristic of aragonite. Prior to shell deposition at 21 hpf, no evidence for carbonates was observed through in vivo CRM.We further analysed the composition of larval shells in three other bivalve species, Mercenaria mercenaria, Crassostrea gigas and Crassostrea virginica and observed no evidence for ACC, which is in contrast to previous work on the same species. Our findings indicate that larval bivalve shells are composed of crystalline aragonite and we demonstrate that conflicting results are related to sub-optimal measurements and misinterpretation of CRM spectra. Our results demonstrate that the common perception that ACC generally occurs as a stable and abundant precursor during larval bivalve calcification needs to be critically reviewed. |
format |
Article in Journal/Newspaper |
author |
Ramesh, Kirti Melzner, Frank Griffith, Andrew W. Gobler, Christopher J. Rouger, Caroline Tasdemir, Deniz Nehrke, Gernot |
spellingShingle |
Ramesh, Kirti Melzner, Frank Griffith, Andrew W. Gobler, Christopher J. Rouger, Caroline Tasdemir, Deniz Nehrke, Gernot In vivo characterization of bivalve larval shells: a confocal Raman microscopy study |
author_facet |
Ramesh, Kirti Melzner, Frank Griffith, Andrew W. Gobler, Christopher J. Rouger, Caroline Tasdemir, Deniz Nehrke, Gernot |
author_sort |
Ramesh, Kirti |
title |
In vivo characterization of bivalve larval shells: a confocal Raman microscopy study |
title_short |
In vivo characterization of bivalve larval shells: a confocal Raman microscopy study |
title_full |
In vivo characterization of bivalve larval shells: a confocal Raman microscopy study |
title_fullStr |
In vivo characterization of bivalve larval shells: a confocal Raman microscopy study |
title_full_unstemmed |
In vivo characterization of bivalve larval shells: a confocal Raman microscopy study |
title_sort |
in vivo characterization of bivalve larval shells: a confocal raman microscopy study |
publisher |
The Royal Society |
publishDate |
2018 |
url |
https://oceanrep.geomar.de/id/eprint/42882/ https://oceanrep.geomar.de/id/eprint/42882/1/Ramesh%20et%20al%202018.pdf https://doi.org/10.1098/rsif.2017.0723 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
https://oceanrep.geomar.de/id/eprint/42882/1/Ramesh%20et%20al%202018.pdf Ramesh, K. , Melzner, F., Griffith, A. W., Gobler, C. J., Rouger, C., Tasdemir, D. and Nehrke, G. (2018) In vivo characterization of bivalve larval shells: a confocal Raman microscopy study . Journal of The Royal Society Interface, 15 (141). p. 20170723. DOI 10.1098/rsif.2017.0723 <https://doi.org/10.1098/rsif.2017.0723>. doi:10.1098/rsif.2017.0723 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1098/rsif.2017.0723 |
container_title |
Journal of The Royal Society Interface |
container_volume |
15 |
container_issue |
141 |
container_start_page |
20170723 |
_version_ |
1766394446699560960 |