Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment
The responses of macroalgae to ocean acidification could be altered by availability of macronutrients, such as ammonium (NH4+). This study determined how the opportunistic macroalga, Ulva australis responded to simultaneous changes in decreasing pH and NH4+ enrichment. This was investigated in a wee...
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Online Access: | https://oceanrep.geomar.de/id/eprint/40506/ https://oceanrep.geomar.de/id/eprint/40506/1/journal.pone.0188389.pdf https://doi.org/10.1371/journal.pone.0188389 |
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ftoceanrep:oai:oceanrep.geomar.de:40506 2023-05-15T17:51:53+02:00 Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment Reidenbach, Leah B. Fernandez, Pamela A. Leal, Pablo P. Noisette, Fanny McGraw, Christina M. Revill, Andrew T. Hurd, Catriona L. Kübler, Janet E. 2017-11-27 text https://oceanrep.geomar.de/id/eprint/40506/ https://oceanrep.geomar.de/id/eprint/40506/1/journal.pone.0188389.pdf https://doi.org/10.1371/journal.pone.0188389 en eng Public Library of Science https://oceanrep.geomar.de/id/eprint/40506/1/journal.pone.0188389.pdf Reidenbach, L. B., Fernandez, P. A., Leal, P. P., Noisette, F., McGraw, C. M., Revill, A. T., Hurd, C. L. and Kübler, J. E. (2017) Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment. Open Access PLoS ONE, 12 (11). e0188389. DOI 10.1371/journal.pone.0188389 <https://doi.org/10.1371/journal.pone.0188389>. doi:10.1371/journal.pone.0188389 cc_by_4.0 info:eu-repo/semantics/openAccess Article PeerReviewed 2017 ftoceanrep https://doi.org/10.1371/journal.pone.0188389 2023-04-07T15:36:56Z The responses of macroalgae to ocean acidification could be altered by availability of macronutrients, such as ammonium (NH4+). This study determined how the opportunistic macroalga, Ulva australis responded to simultaneous changes in decreasing pH and NH4+ enrichment. This was investigated in a week-long growth experiment across a range of predicted future pHs with ambient and enriched NH4+ treatments followed by measurements of relative growth rates (RGR), NH4+ uptake rates and pools, total chlorophyll, and tissue carbon and nitrogen content. Rapid light curves (RLCs) were used to measure the maximum relative electron transport rate (rETRmax) and maximum quantum yield of photosystem II (PSII) photochemistry (Fv/Fm). Photosynthetic capacity was derived from the RLCs and included the efficiency of light harvesting (α), slope of photoinhibition (β), and the light saturation point (Ek). The results showed that NH4+ enrichment did not modify the effects of pH on RGRs, NH4+ uptake rates and pools, total chlorophyll, rETRmax, α, β, Fv/Fm, tissue C and N, and the C:N ratio. However, Ek was differentially affected by pH under different NH4+ treatments. Ek increased with decreasing pH in the ambient NH4+ treatment, but not in the enriched NH4+ treatment. NH4+ enrichment increased RGRs, NH4+ pools, total chlorophyll, rETRmax, α, β, Fv/Fm, and tissue N, and decreased NH4+ uptake rates and the C:N ratio. Decreased pH increased total chlorophyll content, rETRmax, Fv/Fm, and tissue N content, and decreased the C:N ratio. Therefore, the results indicate that U. australis growth is increased with NH4+ enrichment and not with decreasing pH. While decreasing pH influenced the carbon and nitrogen metabolisms of U. australis, it did not result in changes in growth. Article in Journal/Newspaper Ocean acidification OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) PLOS ONE 12 11 e0188389 |
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Open Polar |
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OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
The responses of macroalgae to ocean acidification could be altered by availability of macronutrients, such as ammonium (NH4+). This study determined how the opportunistic macroalga, Ulva australis responded to simultaneous changes in decreasing pH and NH4+ enrichment. This was investigated in a week-long growth experiment across a range of predicted future pHs with ambient and enriched NH4+ treatments followed by measurements of relative growth rates (RGR), NH4+ uptake rates and pools, total chlorophyll, and tissue carbon and nitrogen content. Rapid light curves (RLCs) were used to measure the maximum relative electron transport rate (rETRmax) and maximum quantum yield of photosystem II (PSII) photochemistry (Fv/Fm). Photosynthetic capacity was derived from the RLCs and included the efficiency of light harvesting (α), slope of photoinhibition (β), and the light saturation point (Ek). The results showed that NH4+ enrichment did not modify the effects of pH on RGRs, NH4+ uptake rates and pools, total chlorophyll, rETRmax, α, β, Fv/Fm, tissue C and N, and the C:N ratio. However, Ek was differentially affected by pH under different NH4+ treatments. Ek increased with decreasing pH in the ambient NH4+ treatment, but not in the enriched NH4+ treatment. NH4+ enrichment increased RGRs, NH4+ pools, total chlorophyll, rETRmax, α, β, Fv/Fm, and tissue N, and decreased NH4+ uptake rates and the C:N ratio. Decreased pH increased total chlorophyll content, rETRmax, Fv/Fm, and tissue N content, and decreased the C:N ratio. Therefore, the results indicate that U. australis growth is increased with NH4+ enrichment and not with decreasing pH. While decreasing pH influenced the carbon and nitrogen metabolisms of U. australis, it did not result in changes in growth. |
format |
Article in Journal/Newspaper |
author |
Reidenbach, Leah B. Fernandez, Pamela A. Leal, Pablo P. Noisette, Fanny McGraw, Christina M. Revill, Andrew T. Hurd, Catriona L. Kübler, Janet E. |
spellingShingle |
Reidenbach, Leah B. Fernandez, Pamela A. Leal, Pablo P. Noisette, Fanny McGraw, Christina M. Revill, Andrew T. Hurd, Catriona L. Kübler, Janet E. Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment |
author_facet |
Reidenbach, Leah B. Fernandez, Pamela A. Leal, Pablo P. Noisette, Fanny McGraw, Christina M. Revill, Andrew T. Hurd, Catriona L. Kübler, Janet E. |
author_sort |
Reidenbach, Leah B. |
title |
Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment |
title_short |
Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment |
title_full |
Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment |
title_fullStr |
Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment |
title_full_unstemmed |
Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment |
title_sort |
growth, ammonium metabolism, and photosynthetic properties of ulva australis (chlorophyta) under decreasing ph and ammonium enrichment |
publisher |
Public Library of Science |
publishDate |
2017 |
url |
https://oceanrep.geomar.de/id/eprint/40506/ https://oceanrep.geomar.de/id/eprint/40506/1/journal.pone.0188389.pdf https://doi.org/10.1371/journal.pone.0188389 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://oceanrep.geomar.de/id/eprint/40506/1/journal.pone.0188389.pdf Reidenbach, L. B., Fernandez, P. A., Leal, P. P., Noisette, F., McGraw, C. M., Revill, A. T., Hurd, C. L. and Kübler, J. E. (2017) Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment. Open Access PLoS ONE, 12 (11). e0188389. DOI 10.1371/journal.pone.0188389 <https://doi.org/10.1371/journal.pone.0188389>. doi:10.1371/journal.pone.0188389 |
op_rights |
cc_by_4.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0188389 |
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PLOS ONE |
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12 |
container_issue |
11 |
container_start_page |
e0188389 |
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1766159165867163648 |