Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations
Mixed cultures of 4 polar diatoms regularly found in Antarctic pack ice were grown over 20 d in closed bottles at high light (200 to 250 umol photons m-2 s-1) and at 0*C in order to investigate growth physiology and biomass production under conditions simulating the sea ice habitat during summer. Sp...
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Inter Research
1996
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Online Access: | https://oceanrep.geomar.de/id/eprint/3937/ https://oceanrep.geomar.de/id/eprint/3937/1/m135p169.pdf https://doi.org/10.3354/meps135169 |
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ftoceanrep:oai:oceanrep.geomar.de:3937 2023-05-15T14:08:51+02:00 Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations Gleitz, Markus Kukert, Helmut Riebesell, Ulf Dieckmann, Gerhard S. 1996 text https://oceanrep.geomar.de/id/eprint/3937/ https://oceanrep.geomar.de/id/eprint/3937/1/m135p169.pdf https://doi.org/10.3354/meps135169 en eng Inter Research https://oceanrep.geomar.de/id/eprint/3937/1/m135p169.pdf Gleitz, M., Kukert, H., Riebesell, U. and Dieckmann, G. S. (1996) Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations. Open Access Marine Ecology Progress Series, 135 . pp. 169-177. DOI 10.3354/meps135169 <https://doi.org/10.3354/meps135169>. doi:10.3354/meps135169 info:eu-repo/semantics/openAccess Article PeerReviewed 1996 ftoceanrep https://doi.org/10.3354/meps135169 2023-04-07T14:48:44Z Mixed cultures of 4 polar diatoms regularly found in Antarctic pack ice were grown over 20 d in closed bottles at high light (200 to 250 umol photons m-2 s-1) and at 0*C in order to investigate growth physiology and biomass production under conditions simulating the sea ice habitat during summer. Species tested were: Chaetoceros cf. neogracile, Fragilariopsiscylindrus, Thalassiosiraantarctica and Porosira pseudodenticulata. Initially, all species grew exponentially, but exponential growth ceased for P. pseudodenticulata and T.antarctica after 6 d, for F.cylindrus after 8 d, and for C. cf. neogracile after 10 d. Slight increases in cell number were observed for all species 2 d later. Peak biomass amounted to 140 ug chl a (850 umol particulate organic carbon, POC) l-1. At the same time, concentrations of dissolved inorganic carbon (DIC) were reduced by 1000 uM, oxygen concentrations increased to 1400 uM, and pH increased to 10.5. At this stage, a substantial decline in plasma-containing cells was recorded for F.cylindrus. C. cf. neogracile accounted for 80%, and C. cf. neogracile and F.cylindrus accounted for >95% of total carbon biomass. The carbon isotope composition of POC (expressed as delta13C) increased from -24 to -9 during the experiment. Model calculations showed that diffusive uptake of dissolved CO2 satisfied cellular carbon demand for all species except P. pseudodenticulata at CO2(aq) concentrations >0.5 uM, whereas direct HCO3- utilization was observed for C. cf. neogracile below this concentration. Our data confirm that intense photosynthetic carbon assimilation may lead to profound chemical changes in isolated interstitial brine solutions, with significant consequences for sea ice biota. We propose that the capacity to efficiently utilize ambient DIC, possibly mediated by virtue of favorable surface to volume ratios as well as active pathways of inorganic carbon acquisition, favors growth of small diatoms, and may be an important factor driving ice algal species succession during summer ... Article in Journal/Newspaper Antarc* Antarctic Sea ice OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Antarctic Marine Ecology Progress Series 135 169 177 |
institution |
Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
Mixed cultures of 4 polar diatoms regularly found in Antarctic pack ice were grown over 20 d in closed bottles at high light (200 to 250 umol photons m-2 s-1) and at 0*C in order to investigate growth physiology and biomass production under conditions simulating the sea ice habitat during summer. Species tested were: Chaetoceros cf. neogracile, Fragilariopsiscylindrus, Thalassiosiraantarctica and Porosira pseudodenticulata. Initially, all species grew exponentially, but exponential growth ceased for P. pseudodenticulata and T.antarctica after 6 d, for F.cylindrus after 8 d, and for C. cf. neogracile after 10 d. Slight increases in cell number were observed for all species 2 d later. Peak biomass amounted to 140 ug chl a (850 umol particulate organic carbon, POC) l-1. At the same time, concentrations of dissolved inorganic carbon (DIC) were reduced by 1000 uM, oxygen concentrations increased to 1400 uM, and pH increased to 10.5. At this stage, a substantial decline in plasma-containing cells was recorded for F.cylindrus. C. cf. neogracile accounted for 80%, and C. cf. neogracile and F.cylindrus accounted for >95% of total carbon biomass. The carbon isotope composition of POC (expressed as delta13C) increased from -24 to -9 during the experiment. Model calculations showed that diffusive uptake of dissolved CO2 satisfied cellular carbon demand for all species except P. pseudodenticulata at CO2(aq) concentrations >0.5 uM, whereas direct HCO3- utilization was observed for C. cf. neogracile below this concentration. Our data confirm that intense photosynthetic carbon assimilation may lead to profound chemical changes in isolated interstitial brine solutions, with significant consequences for sea ice biota. We propose that the capacity to efficiently utilize ambient DIC, possibly mediated by virtue of favorable surface to volume ratios as well as active pathways of inorganic carbon acquisition, favors growth of small diatoms, and may be an important factor driving ice algal species succession during summer ... |
format |
Article in Journal/Newspaper |
author |
Gleitz, Markus Kukert, Helmut Riebesell, Ulf Dieckmann, Gerhard S. |
spellingShingle |
Gleitz, Markus Kukert, Helmut Riebesell, Ulf Dieckmann, Gerhard S. Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations |
author_facet |
Gleitz, Markus Kukert, Helmut Riebesell, Ulf Dieckmann, Gerhard S. |
author_sort |
Gleitz, Markus |
title |
Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations |
title_short |
Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations |
title_full |
Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations |
title_fullStr |
Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations |
title_full_unstemmed |
Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations |
title_sort |
carbon acquisition and growth of antarctic sea ice diatoms in closed bottle incubations |
publisher |
Inter Research |
publishDate |
1996 |
url |
https://oceanrep.geomar.de/id/eprint/3937/ https://oceanrep.geomar.de/id/eprint/3937/1/m135p169.pdf https://doi.org/10.3354/meps135169 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Sea ice |
genre_facet |
Antarc* Antarctic Sea ice |
op_relation |
https://oceanrep.geomar.de/id/eprint/3937/1/m135p169.pdf Gleitz, M., Kukert, H., Riebesell, U. and Dieckmann, G. S. (1996) Carbon acquisition and growth of Antarctic sea ice diatoms in closed bottle incubations. Open Access Marine Ecology Progress Series, 135 . pp. 169-177. DOI 10.3354/meps135169 <https://doi.org/10.3354/meps135169>. doi:10.3354/meps135169 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3354/meps135169 |
container_title |
Marine Ecology Progress Series |
container_volume |
135 |
container_start_page |
169 |
op_container_end_page |
177 |
_version_ |
1766280901443977216 |