Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Temperature is important for optimization of rearing conditions in aquaculture, especially during the critical early life history stages of fish. Here, we experimentally investigated the impact of temperature (16, 18, 20, 22 and 24°C) on thermally induced phenotypic variability, from larval hatch to...
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Online Access: | https://oceanrep.geomar.de/id/eprint/39286/ https://oceanrep.geomar.de/id/eprint/39286/1/journal.pone.0182726.pdf https://doi.org/10.1371/journal.pone.0182726 |
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ftoceanrep:oai:oceanrep.geomar.de:39286 2023-05-15T13:27:30+02:00 Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Sørensen, Sune R. Tomkiewicz, Jonna Butts, Ian A. E. 2017-08-14 text https://oceanrep.geomar.de/id/eprint/39286/ https://oceanrep.geomar.de/id/eprint/39286/1/journal.pone.0182726.pdf https://doi.org/10.1371/journal.pone.0182726 en eng Public Library of Science https://oceanrep.geomar.de/id/eprint/39286/1/journal.pone.0182726.pdf Politis, S. N., Mazurais, D., Servili, A., Zambonino-Infante, J. L., Miest, J. J., Sørensen, S. R., Tomkiewicz, J. and Butts, I. A. E. (2017) Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae. Open Access PLoS ONE, 12 (8). e0182726. DOI 10.1371/journal.pone.0182726 <https://doi.org/10.1371/journal.pone.0182726>. doi:10.1371/journal.pone.0182726 cc_by_4.0 info:eu-repo/semantics/openAccess Article PeerReviewed 2017 ftoceanrep https://doi.org/10.1371/journal.pone.0182726 2023-04-07T15:35:04Z Temperature is important for optimization of rearing conditions in aquaculture, especially during the critical early life history stages of fish. Here, we experimentally investigated the impact of temperature (16, 18, 20, 22 and 24°C) on thermally induced phenotypic variability, from larval hatch to first-feeding, and the linked expression of targeted genes [heat shock proteins (hsp), growth hormone (gh) and insulin-like growth factors (igf)] associated to larval performance of European eel, Anguilla anguilla. Temperature effects on larval morphology and gene expression were investigated throughout early larval development (in real time from 0 to 18 days post hatch) and at specific developmental stages (hatch, jaw/teeth formation, and first-feeding). Results showed that hatch success, yolk utilization efficiency, survival, deformities, yolk utilization, and growth rates were all significantly affected by temperature. In real time, increasing temperature from 16 to 22°C accelerated larval development, while larval gene expression patterns (hsp70, hsp90, gh and igf-1) were delayed at cold temperatures (16°C) or accelerated at warm temperatures (20–22°C). All targeted genes (hsp70, hsp90, gh, igf-1, igf-2a, igf-2b) were differentially expressed during larval development. Moreover, expression of gh was highest at 16°C during the jaw/teeth formation, and the first-feeding developmental stages, while expression of hsp90 was highest at 22°C, suggesting thermal stress. Furthermore, 24°C was shown to be deleterious (resulting in 100% mortality), while 16°C and 22°C (~50 and 90% deformities respectively) represent the lower and upper thermal tolerance limits. In conclusion, the high survival, lowest incidence of deformities at hatch, high yolk utilization efficiency, high gh and low hsp expression, suggest 18°C as the optimal temperature for offspring of European eel. Furthermore, our results suggest that the still enigmatic early life history stages of European eel may inhabit the deeper layer of the Sargasso Sea and ... Article in Journal/Newspaper Anguilla anguilla OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) PLOS ONE 12 8 e0182726 |
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Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
Temperature is important for optimization of rearing conditions in aquaculture, especially during the critical early life history stages of fish. Here, we experimentally investigated the impact of temperature (16, 18, 20, 22 and 24°C) on thermally induced phenotypic variability, from larval hatch to first-feeding, and the linked expression of targeted genes [heat shock proteins (hsp), growth hormone (gh) and insulin-like growth factors (igf)] associated to larval performance of European eel, Anguilla anguilla. Temperature effects on larval morphology and gene expression were investigated throughout early larval development (in real time from 0 to 18 days post hatch) and at specific developmental stages (hatch, jaw/teeth formation, and first-feeding). Results showed that hatch success, yolk utilization efficiency, survival, deformities, yolk utilization, and growth rates were all significantly affected by temperature. In real time, increasing temperature from 16 to 22°C accelerated larval development, while larval gene expression patterns (hsp70, hsp90, gh and igf-1) were delayed at cold temperatures (16°C) or accelerated at warm temperatures (20–22°C). All targeted genes (hsp70, hsp90, gh, igf-1, igf-2a, igf-2b) were differentially expressed during larval development. Moreover, expression of gh was highest at 16°C during the jaw/teeth formation, and the first-feeding developmental stages, while expression of hsp90 was highest at 22°C, suggesting thermal stress. Furthermore, 24°C was shown to be deleterious (resulting in 100% mortality), while 16°C and 22°C (~50 and 90% deformities respectively) represent the lower and upper thermal tolerance limits. In conclusion, the high survival, lowest incidence of deformities at hatch, high yolk utilization efficiency, high gh and low hsp expression, suggest 18°C as the optimal temperature for offspring of European eel. Furthermore, our results suggest that the still enigmatic early life history stages of European eel may inhabit the deeper layer of the Sargasso Sea and ... |
format |
Article in Journal/Newspaper |
author |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Sørensen, Sune R. Tomkiewicz, Jonna Butts, Ian A. E. |
spellingShingle |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Sørensen, Sune R. Tomkiewicz, Jonna Butts, Ian A. E. Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae |
author_facet |
Politis, Sebastian N. Mazurais, David Servili, Arianna Zambonino-Infante, Jose-Luis Miest, Joanna J. Sørensen, Sune R. Tomkiewicz, Jonna Butts, Ian A. E. |
author_sort |
Politis, Sebastian N. |
title |
Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae |
title_short |
Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae |
title_full |
Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae |
title_fullStr |
Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae |
title_full_unstemmed |
Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae |
title_sort |
temperature effects on gene expression and morphological development of european eel, anguilla anguilla larvae |
publisher |
Public Library of Science |
publishDate |
2017 |
url |
https://oceanrep.geomar.de/id/eprint/39286/ https://oceanrep.geomar.de/id/eprint/39286/1/journal.pone.0182726.pdf https://doi.org/10.1371/journal.pone.0182726 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
https://oceanrep.geomar.de/id/eprint/39286/1/journal.pone.0182726.pdf Politis, S. N., Mazurais, D., Servili, A., Zambonino-Infante, J. L., Miest, J. J., Sørensen, S. R., Tomkiewicz, J. and Butts, I. A. E. (2017) Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae. Open Access PLoS ONE, 12 (8). e0182726. DOI 10.1371/journal.pone.0182726 <https://doi.org/10.1371/journal.pone.0182726>. doi:10.1371/journal.pone.0182726 |
op_rights |
cc_by_4.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0182726 |
container_title |
PLOS ONE |
container_volume |
12 |
container_issue |
8 |
container_start_page |
e0182726 |
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1766398753337507840 |