New microsatellite markers for the snow crab Chionoecetes opilio (Brachyura: Majidae)

• Published in: Molecular Ecology Notes
• Main Authors: Puebla, Oscar, Parent, E., Sevigny, J.-M.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2003
• Subjects: Chionoecetes opilio; Northwest Atlantic; Snow crab
• Online Access: https://oceanrep.geomar.de/id/eprint/29664/; https://oceanrep.geomar.de/id/eprint/29664/1/Puebla_et_al-2003-Molecular_Ecology_Notes.pdf; https://doi.org/10.1046/j.1471-8286.2003.00542.x

Five microsatellite markers were developed for the snow crab (Chionoecetes opilio) and polymerase chain reaction conditions and/or primer sequences of three previously developed microsatellites were adapted for fluorescent labelling analysis. All loci were analysed in 449 individuals from 11 sampling sites in the northwest Atlantic. The high degree of polymorphism exhibited by these microsatellites (mean of 34 alleles per locus and mean observed heterozygosity of 0.76) suggests that they will be suitable for spatial and temporal genetic analysis of C. opilio populations.