Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea
The Siberian Laptev Sea shelf contains submarine permafrost, which was formed by flooding of terrestrial permafrost with ocean water during the Holocene sea level rise. This flooding resulted in a warming of the permafrost to temperatures close below 0°C. The impact of these environmental changes on...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2009
|
Subjects: | |
Online Access: | https://oceanrep.geomar.de/id/eprint/28415/ https://oceanrep.geomar.de/id/eprint/28415/1/2009_Koch-etal-Methanogenic_EnvirMicBio-11.pdf https://doi.org/10.1111/j.1462-2920.2008.01836.x |
id |
ftoceanrep:oai:oceanrep.geomar.de:28415 |
---|---|
record_format |
openpolar |
spelling |
ftoceanrep:oai:oceanrep.geomar.de:28415 2023-05-15T17:07:12+02:00 Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea Koch, Katharina Knoblauch, Christian Wagner, Dirk 2009 text https://oceanrep.geomar.de/id/eprint/28415/ https://oceanrep.geomar.de/id/eprint/28415/1/2009_Koch-etal-Methanogenic_EnvirMicBio-11.pdf https://doi.org/10.1111/j.1462-2920.2008.01836.x en eng Wiley https://oceanrep.geomar.de/id/eprint/28415/1/2009_Koch-etal-Methanogenic_EnvirMicBio-11.pdf Koch, K., Knoblauch, C. and Wagner, D. (2009) Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea. Open Access Environmental Microbiology, 11 (3). pp. 657-668. DOI 10.1111/j.1462-2920.2008.01836.x <https://doi.org/10.1111/j.1462-2920.2008.01836.x>. doi:10.1111/j.1462-2920.2008.01836.x cc_by_nc_3.0 info:eu-repo/semantics/openAccess Article PeerReviewed 2009 ftoceanrep https://doi.org/10.1111/j.1462-2920.2008.01836.x 2023-04-07T15:18:46Z The Siberian Laptev Sea shelf contains submarine permafrost, which was formed by flooding of terrestrial permafrost with ocean water during the Holocene sea level rise. This flooding resulted in a warming of the permafrost to temperatures close below 0°C. The impact of these environmental changes on methanogenic communities and carbon dynamics in the permafrost was studied in a submarine permafrost core of the Siberian Laptev Sea shelf. Total organic carbon (TOC) content varied between 0.03% and 8.7% with highest values between 53 and 62 m depth below sea floor. In the same depth, maximum methane concentrations (284 nmol CH4 g−1) and lowest carbon isotope values of methane (−72.2‰ VPDB) were measured, latter indicating microbial formation of methane under in situ conditions. The archaeal community structure was assessed by a nested polymerase chain reaction (PCR) amplification for DGGE, followed by sequencing of reamplified bands. Submarine permafrost samples showed a different archaeal community than the nearby terrestrial permafrost. Samples with high methane concentrations were dominated by sequences affiliated rather to the methylotrophic genera Methanosarcina and Methanococcoides as well as to uncultured archaea. The presented results give the first insights into the archaeal community in submarine permafrost and the first evidence for their activity at in situ conditions. Article in Journal/Newspaper laptev Laptev Sea permafrost OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Laptev Sea Environmental Microbiology 11 3 657 668 |
institution |
Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
The Siberian Laptev Sea shelf contains submarine permafrost, which was formed by flooding of terrestrial permafrost with ocean water during the Holocene sea level rise. This flooding resulted in a warming of the permafrost to temperatures close below 0°C. The impact of these environmental changes on methanogenic communities and carbon dynamics in the permafrost was studied in a submarine permafrost core of the Siberian Laptev Sea shelf. Total organic carbon (TOC) content varied between 0.03% and 8.7% with highest values between 53 and 62 m depth below sea floor. In the same depth, maximum methane concentrations (284 nmol CH4 g−1) and lowest carbon isotope values of methane (−72.2‰ VPDB) were measured, latter indicating microbial formation of methane under in situ conditions. The archaeal community structure was assessed by a nested polymerase chain reaction (PCR) amplification for DGGE, followed by sequencing of reamplified bands. Submarine permafrost samples showed a different archaeal community than the nearby terrestrial permafrost. Samples with high methane concentrations were dominated by sequences affiliated rather to the methylotrophic genera Methanosarcina and Methanococcoides as well as to uncultured archaea. The presented results give the first insights into the archaeal community in submarine permafrost and the first evidence for their activity at in situ conditions. |
format |
Article in Journal/Newspaper |
author |
Koch, Katharina Knoblauch, Christian Wagner, Dirk |
spellingShingle |
Koch, Katharina Knoblauch, Christian Wagner, Dirk Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea |
author_facet |
Koch, Katharina Knoblauch, Christian Wagner, Dirk |
author_sort |
Koch, Katharina |
title |
Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea |
title_short |
Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea |
title_full |
Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea |
title_fullStr |
Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea |
title_full_unstemmed |
Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea |
title_sort |
methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the siberian laptev sea |
publisher |
Wiley |
publishDate |
2009 |
url |
https://oceanrep.geomar.de/id/eprint/28415/ https://oceanrep.geomar.de/id/eprint/28415/1/2009_Koch-etal-Methanogenic_EnvirMicBio-11.pdf https://doi.org/10.1111/j.1462-2920.2008.01836.x |
geographic |
Laptev Sea |
geographic_facet |
Laptev Sea |
genre |
laptev Laptev Sea permafrost |
genre_facet |
laptev Laptev Sea permafrost |
op_relation |
https://oceanrep.geomar.de/id/eprint/28415/1/2009_Koch-etal-Methanogenic_EnvirMicBio-11.pdf Koch, K., Knoblauch, C. and Wagner, D. (2009) Methanogenic community composition and anaerobic carbon turnover in submarine permafrost sediments of the Siberian Laptev Sea. Open Access Environmental Microbiology, 11 (3). pp. 657-668. DOI 10.1111/j.1462-2920.2008.01836.x <https://doi.org/10.1111/j.1462-2920.2008.01836.x>. doi:10.1111/j.1462-2920.2008.01836.x |
op_rights |
cc_by_nc_3.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/j.1462-2920.2008.01836.x |
container_title |
Environmental Microbiology |
container_volume |
11 |
container_issue |
3 |
container_start_page |
657 |
op_container_end_page |
668 |
_version_ |
1766062488497946624 |