Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing
Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom...
Published in: | Polar Research |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Norwegian Polar Institute
2014
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Online Access: | https://oceanrep.geomar.de/id/eprint/28020/ https://oceanrep.geomar.de/id/eprint/28020/1/2014_Kiias-etal-Insight_PolarRes-33_23466-141298-2-PB.pdf https://oceanrep.geomar.de/id/eprint/28020/2/2014_Kilias-etal-Insight_PolRes-33_23466-141325-1-SP.pdf https://doi.org/10.3402/polar.v33.23466 |
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ftoceanrep:oai:oceanrep.geomar.de:28020 2023-05-15T14:26:56+02:00 Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing Kilias, Estelle Silvia Peeken, Ilka Metfies, Katja 2014 text https://oceanrep.geomar.de/id/eprint/28020/ https://oceanrep.geomar.de/id/eprint/28020/1/2014_Kiias-etal-Insight_PolarRes-33_23466-141298-2-PB.pdf https://oceanrep.geomar.de/id/eprint/28020/2/2014_Kilias-etal-Insight_PolRes-33_23466-141325-1-SP.pdf https://doi.org/10.3402/polar.v33.23466 en eng Norwegian Polar Institute https://oceanrep.geomar.de/id/eprint/28020/1/2014_Kiias-etal-Insight_PolarRes-33_23466-141298-2-PB.pdf https://oceanrep.geomar.de/id/eprint/28020/2/2014_Kilias-etal-Insight_PolRes-33_23466-141325-1-SP.pdf Kilias, E. S., Peeken, I. and Metfies, K. (2014) Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing. Polar Research, 33 (23466). DOI 10.3402/polar.v33.23466 <https://doi.org/10.3402/polar.v33.23466>. doi:10.3402/polar.v33.23466 cc_by_nc Article PeerReviewed 2014 ftoceanrep https://doi.org/10.3402/polar.v33.23466 2023-04-07T15:18:21Z Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom layer of the sea-ice and melt-pond aggregate were investigated using flow cytometry and 454-pyrosequencing. The objective is to provide a first molecular overview of protist biodiversity in these habitats and to consider the overlaps and/or differences in the community compositions. Results of flow cytometry pointed to a cell size distribution that was dominated by 3–10 µm nanoflagellates. The phylogenetic classification of all sequences was conducted at a high taxonomic level, while a selection of abundant (≥1% of total reads) sequences was further classified at a lower level. At a high taxonomic level, both habitats showed very similar community structures, dominated by chrysophytes and chlorophytes. At a lower taxonomic level, dissimilarities in the diversity of both groups were encountered in the abundant biosphere. While sea-ice chlorophytes and chrysophytes were dominated by Chlamydomonas/Chloromonas spp. and Ochromonas spp., the melt-pond aggregate was dominated by Carteria sp., Ochromonas spp. and Dinobryon faculiferum. We suppose that the similarities in richness and community structure are a consequence of melt-pond freshwater seeping through porous sea ice in late summer. Differences in the abundant biosphere nevertheless indicate that environmental conditions in both habitats vary enough to select for different dominant species. Article in Journal/Newspaper Arctic Arctic Arctic Ocean Polar Research Sea ice OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Arctic Arctic Ocean Polar Research 33 1 23466 |
institution |
Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom layer of the sea-ice and melt-pond aggregate were investigated using flow cytometry and 454-pyrosequencing. The objective is to provide a first molecular overview of protist biodiversity in these habitats and to consider the overlaps and/or differences in the community compositions. Results of flow cytometry pointed to a cell size distribution that was dominated by 3–10 µm nanoflagellates. The phylogenetic classification of all sequences was conducted at a high taxonomic level, while a selection of abundant (≥1% of total reads) sequences was further classified at a lower level. At a high taxonomic level, both habitats showed very similar community structures, dominated by chrysophytes and chlorophytes. At a lower taxonomic level, dissimilarities in the diversity of both groups were encountered in the abundant biosphere. While sea-ice chlorophytes and chrysophytes were dominated by Chlamydomonas/Chloromonas spp. and Ochromonas spp., the melt-pond aggregate was dominated by Carteria sp., Ochromonas spp. and Dinobryon faculiferum. We suppose that the similarities in richness and community structure are a consequence of melt-pond freshwater seeping through porous sea ice in late summer. Differences in the abundant biosphere nevertheless indicate that environmental conditions in both habitats vary enough to select for different dominant species. |
format |
Article in Journal/Newspaper |
author |
Kilias, Estelle Silvia Peeken, Ilka Metfies, Katja |
spellingShingle |
Kilias, Estelle Silvia Peeken, Ilka Metfies, Katja Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
author_facet |
Kilias, Estelle Silvia Peeken, Ilka Metfies, Katja |
author_sort |
Kilias, Estelle Silvia |
title |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_short |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_full |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_fullStr |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_full_unstemmed |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_sort |
insight into protist diversity in arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
publisher |
Norwegian Polar Institute |
publishDate |
2014 |
url |
https://oceanrep.geomar.de/id/eprint/28020/ https://oceanrep.geomar.de/id/eprint/28020/1/2014_Kiias-etal-Insight_PolarRes-33_23466-141298-2-PB.pdf https://oceanrep.geomar.de/id/eprint/28020/2/2014_Kilias-etal-Insight_PolRes-33_23466-141325-1-SP.pdf https://doi.org/10.3402/polar.v33.23466 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Arctic Ocean Polar Research Sea ice |
genre_facet |
Arctic Arctic Arctic Ocean Polar Research Sea ice |
op_relation |
https://oceanrep.geomar.de/id/eprint/28020/1/2014_Kiias-etal-Insight_PolarRes-33_23466-141298-2-PB.pdf https://oceanrep.geomar.de/id/eprint/28020/2/2014_Kilias-etal-Insight_PolRes-33_23466-141325-1-SP.pdf Kilias, E. S., Peeken, I. and Metfies, K. (2014) Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing. Polar Research, 33 (23466). DOI 10.3402/polar.v33.23466 <https://doi.org/10.3402/polar.v33.23466>. doi:10.3402/polar.v33.23466 |
op_rights |
cc_by_nc |
op_doi |
https://doi.org/10.3402/polar.v33.23466 |
container_title |
Polar Research |
container_volume |
33 |
container_issue |
1 |
container_start_page |
23466 |
_version_ |
1766300440358551552 |