Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget
Marine organisms have to cope with increasing CO2 partial pressures and decreasing pH in the oceans. We elucidated the impacts of an 8-week acclimation period to four seawater pCO2 treatments (39, 113, 243 and 405 Pa/385, 1,120, 2,400 and 4,000 µatm) on mantle gene expression patterns in the blue mu...
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Springer
2013
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Online Access: | https://oceanrep.geomar.de/id/eprint/19118/ https://oceanrep.geomar.de/id/eprint/19118/2/227_2012_1930_MOESM1_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/3/227_2012_1930_MOESM2_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/4/227_2012_1930_MOESM3_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/25/art_10.1007_s00227-012-1930-9.pdf https://doi.org/10.1007/s00227-012-1930-9 |
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ftoceanrep:oai:oceanrep.geomar.de:19118 2023-05-15T17:51:37+02:00 Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget Hüning, A. Melzner, Frank Thomsen, F. Gutowska, Magdalena Krämer, L. Rosenstiel, Philip Pörtner, Hans-Otto Philipp, E. E. R. Lucassen, M. 2013 text https://oceanrep.geomar.de/id/eprint/19118/ https://oceanrep.geomar.de/id/eprint/19118/2/227_2012_1930_MOESM1_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/3/227_2012_1930_MOESM2_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/4/227_2012_1930_MOESM3_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/25/art_10.1007_s00227-012-1930-9.pdf https://doi.org/10.1007/s00227-012-1930-9 en eng Springer https://oceanrep.geomar.de/id/eprint/19118/2/227_2012_1930_MOESM1_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/3/227_2012_1930_MOESM2_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/4/227_2012_1930_MOESM3_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/25/art_10.1007_s00227-012-1930-9.pdf Hüning, A., Melzner, F. , Thomsen, F., Gutowska, M., Krämer, L., Rosenstiel, P., Pörtner, H. O., Philipp, E. E. R. and Lucassen, M. (2013) Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget. Marine Biology, 160 (8). pp. 1845-1861. DOI 10.1007/s00227-012-1930-9 <https://doi.org/10.1007/s00227-012-1930-9>. doi:10.1007/s00227-012-1930-9 info:eu-repo/semantics/restrictedAccess Article PeerReviewed 2013 ftoceanrep https://doi.org/10.1007/s00227-012-1930-9 2023-04-07T15:05:59Z Marine organisms have to cope with increasing CO2 partial pressures and decreasing pH in the oceans. We elucidated the impacts of an 8-week acclimation period to four seawater pCO2 treatments (39, 113, 243 and 405 Pa/385, 1,120, 2,400 and 4,000 µatm) on mantle gene expression patterns in the blue mussel Mytilus edulis from the Baltic Sea. Based on the M. edulis mantle tissue transcriptome, the expression of several genes involved in metabolism, calcification and stress responses was assessed in the outer (marginal and pallial zone) and the inner mantle tissues (central zone) using quantitative real-time PCR. The expression of genes involved in energy and protein metabolism (F-ATPase, hexokinase and elongation factor alpha) was strongly affected by acclimation to moderately elevated CO2 partial pressures. Expression of a chitinase, potentially important for the calcification process, was strongly depressed (maximum ninefold), correlating with a linear decrease in shell growth observed in the experimental animals. Interestingly, shell matrix protein candidate genes were less affected by CO2 in both tissues. A compensatory process toward enhanced shell protection is indicated by a massive increase in the expression of tyrosinase, a gene involved in periostracum formation (maximum 220-fold). Using correlation matrices and a force-directed layout network graph, we were able to uncover possible underlying regulatory networks and the connections between different pathways, thereby providing a molecular basis of observed changes in animal physiology in response to ocean acidification. Article in Journal/Newspaper Ocean acidification OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Marine Biology 160 8 1845 1861 |
institution |
Open Polar |
collection |
OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
op_collection_id |
ftoceanrep |
language |
English |
description |
Marine organisms have to cope with increasing CO2 partial pressures and decreasing pH in the oceans. We elucidated the impacts of an 8-week acclimation period to four seawater pCO2 treatments (39, 113, 243 and 405 Pa/385, 1,120, 2,400 and 4,000 µatm) on mantle gene expression patterns in the blue mussel Mytilus edulis from the Baltic Sea. Based on the M. edulis mantle tissue transcriptome, the expression of several genes involved in metabolism, calcification and stress responses was assessed in the outer (marginal and pallial zone) and the inner mantle tissues (central zone) using quantitative real-time PCR. The expression of genes involved in energy and protein metabolism (F-ATPase, hexokinase and elongation factor alpha) was strongly affected by acclimation to moderately elevated CO2 partial pressures. Expression of a chitinase, potentially important for the calcification process, was strongly depressed (maximum ninefold), correlating with a linear decrease in shell growth observed in the experimental animals. Interestingly, shell matrix protein candidate genes were less affected by CO2 in both tissues. A compensatory process toward enhanced shell protection is indicated by a massive increase in the expression of tyrosinase, a gene involved in periostracum formation (maximum 220-fold). Using correlation matrices and a force-directed layout network graph, we were able to uncover possible underlying regulatory networks and the connections between different pathways, thereby providing a molecular basis of observed changes in animal physiology in response to ocean acidification. |
format |
Article in Journal/Newspaper |
author |
Hüning, A. Melzner, Frank Thomsen, F. Gutowska, Magdalena Krämer, L. Rosenstiel, Philip Pörtner, Hans-Otto Philipp, E. E. R. Lucassen, M. |
spellingShingle |
Hüning, A. Melzner, Frank Thomsen, F. Gutowska, Magdalena Krämer, L. Rosenstiel, Philip Pörtner, Hans-Otto Philipp, E. E. R. Lucassen, M. Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget |
author_facet |
Hüning, A. Melzner, Frank Thomsen, F. Gutowska, Magdalena Krämer, L. Rosenstiel, Philip Pörtner, Hans-Otto Philipp, E. E. R. Lucassen, M. |
author_sort |
Hüning, A. |
title |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget |
title_short |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget |
title_full |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget |
title_fullStr |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget |
title_full_unstemmed |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget |
title_sort |
impacts of seawater acidification on mantle gene expression patterns of the baltic sea blue mussel; implications for shell formation and energy budget |
publisher |
Springer |
publishDate |
2013 |
url |
https://oceanrep.geomar.de/id/eprint/19118/ https://oceanrep.geomar.de/id/eprint/19118/2/227_2012_1930_MOESM1_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/3/227_2012_1930_MOESM2_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/4/227_2012_1930_MOESM3_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/25/art_10.1007_s00227-012-1930-9.pdf https://doi.org/10.1007/s00227-012-1930-9 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://oceanrep.geomar.de/id/eprint/19118/2/227_2012_1930_MOESM1_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/3/227_2012_1930_MOESM2_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/4/227_2012_1930_MOESM3_ESM.pdf https://oceanrep.geomar.de/id/eprint/19118/25/art_10.1007_s00227-012-1930-9.pdf Hüning, A., Melzner, F. , Thomsen, F., Gutowska, M., Krämer, L., Rosenstiel, P., Pörtner, H. O., Philipp, E. E. R. and Lucassen, M. (2013) Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget. Marine Biology, 160 (8). pp. 1845-1861. DOI 10.1007/s00227-012-1930-9 <https://doi.org/10.1007/s00227-012-1930-9>. doi:10.1007/s00227-012-1930-9 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00227-012-1930-9 |
container_title |
Marine Biology |
container_volume |
160 |
container_issue |
8 |
container_start_page |
1845 |
op_container_end_page |
1861 |
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1766158817474641920 |