The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric e...
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ftoceanrep:oai:oceanrep.geomar.de:14924 2023-05-15T17:50:30+02:00 The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios Rouco-Molina, Monica Branson, Oscar Lebrato, Mario Iglesias-Rodriguez, Débora 2013-06-18 text https://oceanrep.geomar.de/id/eprint/14924/ https://oceanrep.geomar.de/id/eprint/14924/1/fmicb-04-00155.pdf https://doi.org/10.3389/fmicb.2013.00155 en eng Frontiers https://oceanrep.geomar.de/id/eprint/14924/1/fmicb-04-00155.pdf Rouco-Molina, M., Branson, O., Lebrato, M. and Iglesias-Rodriguez, D. (2013) The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios. Open Access Frontiers in Microbiology, 4 (155). DOI 10.3389/fmicb.2013.00155 <https://doi.org/10.3389/fmicb.2013.00155>. doi:10.3389/fmicb.2013.00155 cc_by info:eu-repo/semantics/openAccess Article PeerReviewed 2013 ftoceanrep https://doi.org/10.3389/fmicb.2013.00155 2023-04-07T15:04:45Z Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 μatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 μatm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification. Article in Journal/Newspaper Ocean acidification OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) Frontiers in Microbiology 4 |
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OceanRep (GEOMAR Helmholtz Centre für Ocean Research Kiel) |
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English |
description |
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 μatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 μatm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification. |
format |
Article in Journal/Newspaper |
author |
Rouco-Molina, Monica Branson, Oscar Lebrato, Mario Iglesias-Rodriguez, Débora |
spellingShingle |
Rouco-Molina, Monica Branson, Oscar Lebrato, Mario Iglesias-Rodriguez, Débora The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios |
author_facet |
Rouco-Molina, Monica Branson, Oscar Lebrato, Mario Iglesias-Rodriguez, Débora |
author_sort |
Rouco-Molina, Monica |
title |
The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios |
title_short |
The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios |
title_full |
The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios |
title_fullStr |
The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios |
title_full_unstemmed |
The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios |
title_sort |
effect of nitrate and phosphate availability on emiliania huxleyi physiology under different co2 scenarios |
publisher |
Frontiers |
publishDate |
2013 |
url |
https://oceanrep.geomar.de/id/eprint/14924/ https://oceanrep.geomar.de/id/eprint/14924/1/fmicb-04-00155.pdf https://doi.org/10.3389/fmicb.2013.00155 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://oceanrep.geomar.de/id/eprint/14924/1/fmicb-04-00155.pdf Rouco-Molina, M., Branson, O., Lebrato, M. and Iglesias-Rodriguez, D. (2013) The effect of nitrate and phosphate availability on Emiliania huxleyi physiology under different CO2 scenarios. Open Access Frontiers in Microbiology, 4 (155). DOI 10.3389/fmicb.2013.00155 <https://doi.org/10.3389/fmicb.2013.00155>. doi:10.3389/fmicb.2013.00155 |
op_rights |
cc_by info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmicb.2013.00155 |
container_title |
Frontiers in Microbiology |
container_volume |
4 |
_version_ |
1766157262355693568 |