Elevated seawater pCO2 differentially affects branchial acid-base transporters over the course of development in the cephalopod Sepia officinalis

• Published in: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
• Main Authors: Hu, Marian Yong-An, Tseng, Yung-Che, Stumpp, Meike, Gutowska, Magdalena, Kiko, Rainer, Lucassen, Magnus, Melzner, Frank
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Physiological Society, APS 2011
• Subjects: Sonka; Ocean acidification
• Online Access: https://oceanrep.geomar.de/id/eprint/12758/; https://oceanrep.geomar.de/id/eprint/12758/1/Elevated.pdf; https://oceanrep.geomar.de/id/eprint/12758/2/Correction_Elevated.pdf; https://doi.org/10.1152/ajpregu.00653.2010

The specific transporters involved in maintenance of blood pH homeostasis in cephalopod molluscs have not been identified to date. Using in situ hybridization and immuno histochemical methods, we demonstrate that Na+/K+-ATPase (soNKA), a V-type H+-ATPase (soV-HA), and Na+/HCO3- cotransporter (soNBC) are co-localized in NKA-rich cells in the gills of Sepia officinalis. mRNA expression patterns of these transporters and selected metabolic genes were examined in response to moderately elevated seawater pCO2 (0.16 and 0.35 kPa) over a time-course of six weeks in different ontogenetic stages. The applied CO2 concentrations are relevant for ocean acidification scenarios projected for the coming decades. We determined strong expression changes in late stage embryos and hatchlings, with one to three log2-fold reductions in soNKA, soNBCe, socCAII and COX. In contrast, no hypercapnia induced changes in mRNA expression were observed in juveniles during both short- and long-term exposure. However a transiently increased demand of ion regulatory demand was evident during the initial acclimation reaction to elevated seawater pCO2. Gill Na+/K+-ATPase activity and protein concentration were increased by approximately 15% in during short (2-11 day), but not long term (42 day) exposure. Our findings support the hypothesis that the energy budget of adult cephalopods is not significantly compromised during long-term exposure to moderate environmental hypercapnia. However, the down regulation of ion-regulatory and metabolic genes in late stage embryos, taken together with a significant reduction in somatic growth, indicates that cephalopod early life stages are challenged by elevated seawater pCO2.