Characterization and isolation of microsatellite in Persian sturgeon (Acipenser persicus, Borodine, 1897)
In order to have a sustainable management on Persian sturgeon (Acipenser persicus) as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for th...
Main Authors: | , , , , , , , , |
---|---|
Format: | Book |
Language: | Persian |
Published: |
Iranian Fisheries Science Research Institute
2013
|
Subjects: | |
Online Access: | http://hdl.handle.net/1834/39810 |
id |
ftoceandocs:oai:aquadocs.org:1834/39810 |
---|---|
record_format |
openpolar |
spelling |
ftoceandocs:oai:aquadocs.org:1834/39810 2023-05-15T15:41:48+02:00 Characterization and isolation of microsatellite in Persian sturgeon (Acipenser persicus, Borodine, 1897) Moghim, Mehdi Porkazemi, M Rezvani Gilkolaei, S. Laloei, F. Bagherzadeh Afrozi, F. Yarmohamadi, M Kor, D. Nayerani, M Taghavi, M.J. 2013 application/pdf 160 http://hdl.handle.net/1834/39810 fa per Iranian Fisheries Science Research Institute Tehran, Iran http://kmsu.ac.ir/ 42343 http://hdl.handle.net/1834/39810 http://aquaticcommons.org/id/eprint/25417 18721 2018-09-23 16:10:44 25417 Iranian Fisheries Science Research Institute Biology Iran Persian sturgeon Acipenser persicus Microsatellite Population Genetic Species Diversity Russian Sturgeon A. gueldenstaedtii A. stellatus A.nudiventris monograph 2013 ftoceandocs 2023-04-06T17:06:31Z In order to have a sustainable management on Persian sturgeon (Acipenser persicus) as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for this all Caspian Sea' sturgeon species it requires knowledge of its genetic diversity using reliable molecular marker to study population genetic structure. For these purposes, an enriched library was prepared based on a modified biotin-capture method. Approximately 1800 positive clones were screened for microsatellites in an Acipenser persicus genomic library. Of these 350 positively hybridizing clones were sequenced, and 81 clones were identified as having microsatellites with adequate flanking regions. We developed and tested 68 microsatellite primer pairs for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii). Several of the markers appeared useful for studies stellate sturgeon (A. stellatus), ship sturgeon (A.nudiventris) and beluga (Huso huso). Nearly all the polymorphic pattern for ship, stellate and beluga displayed the simple banding patterns characteristic of disomic loci, while those for Russian sturgeon displayed banding patterns characteristic of tetraploid or higher polyploid levels. These markers may prove useful in a variety of future sturgeon population genetic studies in the Caspian Sea. Book Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
Persian |
topic |
Biology Iran Persian sturgeon Acipenser persicus Microsatellite Population Genetic Species Diversity Russian Sturgeon A. gueldenstaedtii A. stellatus A.nudiventris |
spellingShingle |
Biology Iran Persian sturgeon Acipenser persicus Microsatellite Population Genetic Species Diversity Russian Sturgeon A. gueldenstaedtii A. stellatus A.nudiventris Moghim, Mehdi Porkazemi, M Rezvani Gilkolaei, S. Laloei, F. Bagherzadeh Afrozi, F. Yarmohamadi, M Kor, D. Nayerani, M Taghavi, M.J. Characterization and isolation of microsatellite in Persian sturgeon (Acipenser persicus, Borodine, 1897) |
topic_facet |
Biology Iran Persian sturgeon Acipenser persicus Microsatellite Population Genetic Species Diversity Russian Sturgeon A. gueldenstaedtii A. stellatus A.nudiventris |
description |
In order to have a sustainable management on Persian sturgeon (Acipenser persicus) as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for this all Caspian Sea' sturgeon species it requires knowledge of its genetic diversity using reliable molecular marker to study population genetic structure. For these purposes, an enriched library was prepared based on a modified biotin-capture method. Approximately 1800 positive clones were screened for microsatellites in an Acipenser persicus genomic library. Of these 350 positively hybridizing clones were sequenced, and 81 clones were identified as having microsatellites with adequate flanking regions. We developed and tested 68 microsatellite primer pairs for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii). Several of the markers appeared useful for studies stellate sturgeon (A. stellatus), ship sturgeon (A.nudiventris) and beluga (Huso huso). Nearly all the polymorphic pattern for ship, stellate and beluga displayed the simple banding patterns characteristic of disomic loci, while those for Russian sturgeon displayed banding patterns characteristic of tetraploid or higher polyploid levels. These markers may prove useful in a variety of future sturgeon population genetic studies in the Caspian Sea. |
format |
Book |
author |
Moghim, Mehdi Porkazemi, M Rezvani Gilkolaei, S. Laloei, F. Bagherzadeh Afrozi, F. Yarmohamadi, M Kor, D. Nayerani, M Taghavi, M.J. |
author_facet |
Moghim, Mehdi Porkazemi, M Rezvani Gilkolaei, S. Laloei, F. Bagherzadeh Afrozi, F. Yarmohamadi, M Kor, D. Nayerani, M Taghavi, M.J. |
author_sort |
Moghim, Mehdi |
title |
Characterization and isolation of microsatellite in Persian sturgeon (Acipenser persicus, Borodine, 1897) |
title_short |
Characterization and isolation of microsatellite in Persian sturgeon (Acipenser persicus, Borodine, 1897) |
title_full |
Characterization and isolation of microsatellite in Persian sturgeon (Acipenser persicus, Borodine, 1897) |
title_fullStr |
Characterization and isolation of microsatellite in Persian sturgeon (Acipenser persicus, Borodine, 1897) |
title_full_unstemmed |
Characterization and isolation of microsatellite in Persian sturgeon (Acipenser persicus, Borodine, 1897) |
title_sort |
characterization and isolation of microsatellite in persian sturgeon (acipenser persicus, borodine, 1897) |
publisher |
Iranian Fisheries Science Research Institute |
publishDate |
2013 |
url |
http://hdl.handle.net/1834/39810 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
http://aquaticcommons.org/id/eprint/25417 18721 2018-09-23 16:10:44 25417 Iranian Fisheries Science Research Institute |
op_relation |
http://kmsu.ac.ir/ 42343 http://hdl.handle.net/1834/39810 |
_version_ |
1766374682614824960 |