Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea
Sturgeon stocks are drastically decreasing in Caspian Sea during recent decades. This project has been proposed to collect the excess sperm of Caspian Sea Southern part sturgeon breeders, to cryopreserve and hold them for further use. During two spawning seasons (2006-2007), sperm quality and quanti...
Main Authors: | , , , , , |
---|---|
Format: | Book |
Language: | Persian |
Published: |
Iranian Fisheries Science Research Institute
2012
|
Subjects: | |
Online Access: | http://hdl.handle.net/1834/39790 |
id |
ftoceandocs:oai:aquadocs.org:1834/39790 |
---|---|
record_format |
openpolar |
spelling |
ftoceandocs:oai:aquadocs.org:1834/39790 2023-05-15T15:41:48+02:00 Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea Baradaran Noveiri, Shahrouz Alipoor, A.R. Mohseni, M. Pordehghani, M. Chakmedooz, F. Halajiyan, A. 2012 application/pdf 50 http://hdl.handle.net/1834/39790 fa per Iranian Fisheries Science Research Institute Tehran, Iran http://kmsu.ac.ir/ 40607 http://hdl.handle.net/1834/39790 http://aquaticcommons.org/id/eprint/25397 18721 2018-09-20 11:42:43 25397 Iranian Fisheries Science Research Institute Aquaculture Iran Caspian Sea Spermatozoa Cryopreservation Percentage of motility Sperm bank Sturgeon Brood stocks Spawning Fertilization Samples Density monograph 2012 ftoceandocs 2023-04-06T17:06:31Z Sturgeon stocks are drastically decreasing in Caspian Sea during recent decades. This project has been proposed to collect the excess sperm of Caspian Sea Southern part sturgeon breeders, to cryopreserve and hold them for further use. During two spawning seasons (2006-2007), sperm quality and quantity analysis were conducted on 34 different male sturgeons of which 12 Persian sturgeon, 4 stellate sturgeon, 1 beluga and 1 ship sturgeon with better quality were chosen to cryopreserve. After dilution of fresh sperm with sturgeon sperm diluent (115 mM Tris, 23.4 mM sucrose and 15% DMSO) at the ratio of 1:1, the 0.5 ml straws were filled using special filling and sealing apparatus and frozen with a multi step freezing rate. To access the further quality and fertilization test, samples were thawed at 40 °C for 20 seconds. During the project performance, 1010 ml of Persian sturgeon sperm , 110 ml of ship sturgeon sperm , 90 ml of stellate and 80 ml of beluga sperm were cryopreserved. The reduction of frozen-thawed sperm motility in comparison with fresh samples showed 10-52.3 % decrease, but there was not any significant differences between samples after 7 months preservation in liquid Nitrogen (P < 0.005). River caught Persian sturgeon samples showed higher sperm density in comparison with nonriverine breeders (P < 0.05). Data showed that stellate sturgeon sperm showed better resistance to freezing condition in comparison with Persian sturgeon spermatozoa. Fertilization tests (4 hours after fertilization) showed 27.4% and 58.2% fertilization rate in frozen-thawed stellate and Persian sturgeon spermatozoa, respectively. Book Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
Persian |
topic |
Aquaculture Iran Caspian Sea Spermatozoa Cryopreservation Percentage of motility Sperm bank Sturgeon Brood stocks Spawning Fertilization Samples Density |
spellingShingle |
Aquaculture Iran Caspian Sea Spermatozoa Cryopreservation Percentage of motility Sperm bank Sturgeon Brood stocks Spawning Fertilization Samples Density Baradaran Noveiri, Shahrouz Alipoor, A.R. Mohseni, M. Pordehghani, M. Chakmedooz, F. Halajiyan, A. Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
topic_facet |
Aquaculture Iran Caspian Sea Spermatozoa Cryopreservation Percentage of motility Sperm bank Sturgeon Brood stocks Spawning Fertilization Samples Density |
description |
Sturgeon stocks are drastically decreasing in Caspian Sea during recent decades. This project has been proposed to collect the excess sperm of Caspian Sea Southern part sturgeon breeders, to cryopreserve and hold them for further use. During two spawning seasons (2006-2007), sperm quality and quantity analysis were conducted on 34 different male sturgeons of which 12 Persian sturgeon, 4 stellate sturgeon, 1 beluga and 1 ship sturgeon with better quality were chosen to cryopreserve. After dilution of fresh sperm with sturgeon sperm diluent (115 mM Tris, 23.4 mM sucrose and 15% DMSO) at the ratio of 1:1, the 0.5 ml straws were filled using special filling and sealing apparatus and frozen with a multi step freezing rate. To access the further quality and fertilization test, samples were thawed at 40 °C for 20 seconds. During the project performance, 1010 ml of Persian sturgeon sperm , 110 ml of ship sturgeon sperm , 90 ml of stellate and 80 ml of beluga sperm were cryopreserved. The reduction of frozen-thawed sperm motility in comparison with fresh samples showed 10-52.3 % decrease, but there was not any significant differences between samples after 7 months preservation in liquid Nitrogen (P < 0.005). River caught Persian sturgeon samples showed higher sperm density in comparison with nonriverine breeders (P < 0.05). Data showed that stellate sturgeon sperm showed better resistance to freezing condition in comparison with Persian sturgeon spermatozoa. Fertilization tests (4 hours after fertilization) showed 27.4% and 58.2% fertilization rate in frozen-thawed stellate and Persian sturgeon spermatozoa, respectively. |
format |
Book |
author |
Baradaran Noveiri, Shahrouz Alipoor, A.R. Mohseni, M. Pordehghani, M. Chakmedooz, F. Halajiyan, A. |
author_facet |
Baradaran Noveiri, Shahrouz Alipoor, A.R. Mohseni, M. Pordehghani, M. Chakmedooz, F. Halajiyan, A. |
author_sort |
Baradaran Noveiri, Shahrouz |
title |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_short |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_full |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_fullStr |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_full_unstemmed |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_sort |
creating a sperm bank from sturgeon brood stocks in the south caspian sea |
publisher |
Iranian Fisheries Science Research Institute |
publishDate |
2012 |
url |
http://hdl.handle.net/1834/39790 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
http://aquaticcommons.org/id/eprint/25397 18721 2018-09-20 11:42:43 25397 Iranian Fisheries Science Research Institute |
op_relation |
http://kmsu.ac.ir/ 40607 http://hdl.handle.net/1834/39790 |
_version_ |
1766374695327760384 |