The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique
The goal of this study was to find a sex-specific DNA sequences in the male and female genomes of beluga (Huso huso), by random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Of the 310 different primers was tested, 7 primers produced no band. The most likely possibility is...
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ftoceandocs:oai:aquadocs.org:1834/39242 2023-05-15T15:41:28+02:00 The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique Keyvan Shokoo, S. Pourkazemi, M. Kalbasi, M.R. 2004 application/pdf 149-162 http://hdl.handle.net/1834/39242 fa per http://www.ifro.ir 1026-1354 http://hdl.handle.net/1834/39242 http://aquaticcommons.org/id/eprint/24828 18721 2018-07-17 01:43:20 24828 Iranian Fisheries Science Research Institute Biology Beluga Huso huso Acipenseridae RAPD Sex marker Caspian Sea Iran article TRUE 2004 ftoceandocs 2023-04-06T17:06:23Z The goal of this study was to find a sex-specific DNA sequences in the male and female genomes of beluga (Huso huso), by random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Of the 310 different primers was tested, 7 primers produced no band. The most likely possibility is that PCR conditions have not been suitable for these 7 primers. It is also possible that these RAPD primers did not find suitable binding position to generate fragments. A single band was produced by primer 295 (OPT -17) which was specific to the male DNA. Further analysis of individual male and female beluga demonstrated that this primer yielded a product that were in fact variable and reproducible, but were the result of variation in some individual males and females and not male-specific. Therefore, of the 4146 RAPDs scored, none were tightly linked to a sex-determining gene. The results support the idea that sex chromosomes are either not present or they weakly differentiated in the genome of beluga. Article in Journal/Newspaper Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
Persian |
topic |
Biology Beluga Huso huso Acipenseridae RAPD Sex marker Caspian Sea Iran |
spellingShingle |
Biology Beluga Huso huso Acipenseridae RAPD Sex marker Caspian Sea Iran Keyvan Shokoo, S. Pourkazemi, M. Kalbasi, M.R. The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
topic_facet |
Biology Beluga Huso huso Acipenseridae RAPD Sex marker Caspian Sea Iran |
description |
The goal of this study was to find a sex-specific DNA sequences in the male and female genomes of beluga (Huso huso), by random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Of the 310 different primers was tested, 7 primers produced no band. The most likely possibility is that PCR conditions have not been suitable for these 7 primers. It is also possible that these RAPD primers did not find suitable binding position to generate fragments. A single band was produced by primer 295 (OPT -17) which was specific to the male DNA. Further analysis of individual male and female beluga demonstrated that this primer yielded a product that were in fact variable and reproducible, but were the result of variation in some individual males and females and not male-specific. Therefore, of the 4146 RAPDs scored, none were tightly linked to a sex-determining gene. The results support the idea that sex chromosomes are either not present or they weakly differentiated in the genome of beluga. |
format |
Article in Journal/Newspaper |
author |
Keyvan Shokoo, S. Pourkazemi, M. Kalbasi, M.R. |
author_facet |
Keyvan Shokoo, S. Pourkazemi, M. Kalbasi, M.R. |
author_sort |
Keyvan Shokoo, S. |
title |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_short |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_full |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_fullStr |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_full_unstemmed |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_sort |
possibility of sex identification in beluga (huso huso) by using pcr-rapd technique |
publishDate |
2004 |
url |
http://hdl.handle.net/1834/39242 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
http://aquaticcommons.org/id/eprint/24828 18721 2018-07-17 01:43:20 24828 Iranian Fisheries Science Research Institute |
op_relation |
http://www.ifro.ir 1026-1354 http://hdl.handle.net/1834/39242 |
_version_ |
1766374371858841600 |