Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique
In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, ge...
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ftoceandocs:oai:aquadocs.org:1834/37291 2023-05-15T15:41:42+02:00 Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. 2012 application/pdf 415-423 http://hdl.handle.net/1834/37291 en eng http://www.ifro.ir 1562-2916 http://hdl.handle.net/1834/37291 http://aquaticcommons.org/id/eprint/22524 18721 2018-04-17 20:06:54 22524 Iranian Fisheries Science Research Institute Biology Fisheries AFLP Hybrid identification Bester Beluga Iran article TRUE 2012 ftoceandocs 2023-04-06T17:05:52Z In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster analyses (UPGMA) allow a correct identification. We used 8 primer combinations (Eco+3, Mse+4) and a total of 250 bands (size range 40-1000 bp) were generated. Primer combinations of (E-AAT, M-CGAT) and (E-AAG, M-CGAT) produced diagnostic bands in hybrids and parental species. Moreover, the results of genetic identification showed that Bester hybrids are more similar to beluga (Huso huso) (0.68) in comparison with sterlet (Acipenser ruthenus) (0.45).The results suggested that this technique could be suitable for precise identification of species and inter-generic hybrids like bester. Article in Journal/Newspaper Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
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Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
English |
topic |
Biology Fisheries AFLP Hybrid identification Bester Beluga Iran |
spellingShingle |
Biology Fisheries AFLP Hybrid identification Bester Beluga Iran Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
topic_facet |
Biology Fisheries AFLP Hybrid identification Bester Beluga Iran |
description |
In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster analyses (UPGMA) allow a correct identification. We used 8 primer combinations (Eco+3, Mse+4) and a total of 250 bands (size range 40-1000 bp) were generated. Primer combinations of (E-AAT, M-CGAT) and (E-AAG, M-CGAT) produced diagnostic bands in hybrids and parental species. Moreover, the results of genetic identification showed that Bester hybrids are more similar to beluga (Huso huso) (0.68) in comparison with sterlet (Acipenser ruthenus) (0.45).The results suggested that this technique could be suitable for precise identification of species and inter-generic hybrids like bester. |
format |
Article in Journal/Newspaper |
author |
Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. |
author_facet |
Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. |
author_sort |
Yarmohammadi, M. |
title |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_short |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_full |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_fullStr |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_full_unstemmed |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_sort |
identification of bester hybrids (female huso huso linnaeus, 1758 and male sterlet acipenser ruthenus linnaeus, 1758) using aflp molecular technique |
publishDate |
2012 |
url |
http://hdl.handle.net/1834/37291 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
http://aquaticcommons.org/id/eprint/22524 18721 2018-04-17 20:06:54 22524 Iranian Fisheries Science Research Institute |
op_relation |
http://www.ifro.ir 1562-2916 http://hdl.handle.net/1834/37291 |
_version_ |
1766374598872399872 |