Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga, Huso huso Linnaeus, 1754
In this study the LC50 96h values of water-soluble fractions (WSF) of the Caspian Sea – exploited crude oil were determined in beluga, Huso huso fingerlings. The fingerlings averaged 2.42 ± 0.11 g in weight were exposed to WSF crude oil at concentrations 24.75, 27, 29.25, 31.5, 33.75, 36, 38.25 ppm....
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ftoceandocs:oai:aquadocs.org:1834/36556 2023-05-15T15:41:41+02:00 Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga, Huso huso Linnaeus, 1754 Khabakhsh, E. Jamili, Sh. Motalebi, A. Mashinchian moradi, A. Nasrolahzade Saravi, H. 2014 application/pdf 63-72 http://hdl.handle.net/1834/36556 en eng http://cjes.guilan.ac.ir/ 1735-3033 http://hdl.handle.net/1834/36556 http://aquaticcommons.org/id/eprint/21733 18721 2017-11-30 03:19:16 21733 University of Guilan, Faculty of Natural Resources, Iran Aquaculture Biology Fisheries histopathological water crude oil liver tissue fingerling beluga Huso huso Caspian sea Iran article TRUE 2014 ftoceandocs 2023-04-06T17:05:41Z In this study the LC50 96h values of water-soluble fractions (WSF) of the Caspian Sea – exploited crude oil were determined in beluga, Huso huso fingerlings. The fingerlings averaged 2.42 ± 0.11 g in weight were exposed to WSF crude oil at concentrations 24.75, 27, 29.25, 31.5, 33.75, 36, 38.25 ppm. The treatments were performed in three replicates and all changes in the specimens were determined for each concentration. Water quality parameters of the seawater were tested and recorded. The LC50 96h value was found to be 34.87 ppm. In the other step, the fingerling beluga was exposed to three sub-lethal concentrations (13.93, 20.92, 27.90 ppm) of WSF of the Caspian crude oil representing 40, 60 and 80% of LC50 96h respectively, and control without crude oil. All treatments and control were conducted in triplicate. Three specimens of each treatment were sampled for histological studies every day. Results revealed that the fish liver tissue of all the treatments showed histological changes, in comparison to the control after 2 days. Even if the changes in the treatment exposed to 27.90 ppm (80% LC50 96h) WSF occurred in 24h after exposure. Histopathological findings in liver included cloudy swelling, pyknotic nuclei, Karyorrhectic nuclei, fatty vacuolation, fatty degeneration, hypertrophy of hepatocyte, necrosis, hypertrophy of bile ducts, cholestasis and bile stagnation, inflammation, fibrosis and cirrhosis. These alterations were more conspicuous with increasing the soluble fraction of crude oil concentrations and exposing time. The results are of importance since much attention has been paid to the oil slicks, particulate or sedimentary pollutions, instead of potential toxic effects of water-soluble fractions of oil, which are more available to marine biota. Article in Journal/Newspaper Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
English |
topic |
Aquaculture Biology Fisheries histopathological water crude oil liver tissue fingerling beluga Huso huso Caspian sea Iran |
spellingShingle |
Aquaculture Biology Fisheries histopathological water crude oil liver tissue fingerling beluga Huso huso Caspian sea Iran Khabakhsh, E. Jamili, Sh. Motalebi, A. Mashinchian moradi, A. Nasrolahzade Saravi, H. Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga, Huso huso Linnaeus, 1754 |
topic_facet |
Aquaculture Biology Fisheries histopathological water crude oil liver tissue fingerling beluga Huso huso Caspian sea Iran |
description |
In this study the LC50 96h values of water-soluble fractions (WSF) of the Caspian Sea – exploited crude oil were determined in beluga, Huso huso fingerlings. The fingerlings averaged 2.42 ± 0.11 g in weight were exposed to WSF crude oil at concentrations 24.75, 27, 29.25, 31.5, 33.75, 36, 38.25 ppm. The treatments were performed in three replicates and all changes in the specimens were determined for each concentration. Water quality parameters of the seawater were tested and recorded. The LC50 96h value was found to be 34.87 ppm. In the other step, the fingerling beluga was exposed to three sub-lethal concentrations (13.93, 20.92, 27.90 ppm) of WSF of the Caspian crude oil representing 40, 60 and 80% of LC50 96h respectively, and control without crude oil. All treatments and control were conducted in triplicate. Three specimens of each treatment were sampled for histological studies every day. Results revealed that the fish liver tissue of all the treatments showed histological changes, in comparison to the control after 2 days. Even if the changes in the treatment exposed to 27.90 ppm (80% LC50 96h) WSF occurred in 24h after exposure. Histopathological findings in liver included cloudy swelling, pyknotic nuclei, Karyorrhectic nuclei, fatty vacuolation, fatty degeneration, hypertrophy of hepatocyte, necrosis, hypertrophy of bile ducts, cholestasis and bile stagnation, inflammation, fibrosis and cirrhosis. These alterations were more conspicuous with increasing the soluble fraction of crude oil concentrations and exposing time. The results are of importance since much attention has been paid to the oil slicks, particulate or sedimentary pollutions, instead of potential toxic effects of water-soluble fractions of oil, which are more available to marine biota. |
format |
Article in Journal/Newspaper |
author |
Khabakhsh, E. Jamili, Sh. Motalebi, A. Mashinchian moradi, A. Nasrolahzade Saravi, H. |
author_facet |
Khabakhsh, E. Jamili, Sh. Motalebi, A. Mashinchian moradi, A. Nasrolahzade Saravi, H. |
author_sort |
Khabakhsh, E. |
title |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga, Huso huso Linnaeus, 1754 |
title_short |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga, Huso huso Linnaeus, 1754 |
title_full |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga, Huso huso Linnaeus, 1754 |
title_fullStr |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga, Huso huso Linnaeus, 1754 |
title_full_unstemmed |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga, Huso huso Linnaeus, 1754 |
title_sort |
histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga, huso huso linnaeus, 1754 |
publishDate |
2014 |
url |
http://hdl.handle.net/1834/36556 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
http://aquaticcommons.org/id/eprint/21733 18721 2017-11-30 03:19:16 21733 University of Guilan, Faculty of Natural Resources, Iran |
op_relation |
http://cjes.guilan.ac.ir/ 1735-3033 http://hdl.handle.net/1834/36556 |
_version_ |
1766374575857205248 |