Study of bacterial agents on external lesions of cultured Sturgeons in Sngar zone of Gilan Province
This study was carried out to isolate and determine bacterial agents in outer lesions of sturgeons in Shahid Dr. Beheshti sturgeon propagation and rearing center in Gilan province. Five species of sturgeons were studied from viewpoint of lesions. A number of 167 specimens of Beluga, 76 specimens of...
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Islamic Azad University, Science and Research Branch, Tehran, Aquatic Animal Health
2009
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ftoceandocs:oai:aquadocs.org:1834/35774 2023-05-15T15:41:55+02:00 Study of bacterial agents on external lesions of cultured Sturgeons in Sngar zone of Gilan Province Seyed Javad, Abolghasemi 2009 application/pdf 135 http://hdl.handle.net/1834/35774 fa per Islamic Azad University, Science and Research Branch, Tehran, Aquatic Animal Health http://www.srbiau.ac.ir/ http://hdl.handle.net/1834/35774 http://aquaticcommons.org/id/eprint/20912 17408 2016-07-15 09:17:40 20912 Islamic Azad University, Science and Research Branch, Tehran Aquaculture thesis 2009 ftoceandocs 2023-04-06T17:05:29Z This study was carried out to isolate and determine bacterial agents in outer lesions of sturgeons in Shahid Dr. Beheshti sturgeon propagation and rearing center in Gilan province. Five species of sturgeons were studied from viewpoint of lesions. A number of 167 specimens of Beluga, 76 specimens of Persian sturgeon, 27 specimens of Russian sturgeon, 42 specimens of stellate and finally 23 specimens of ship had bacterial lesions in different outer parts of their bodies. After sampling and purification, bacterial cultures and biochemical tests were done. After the isolation of bacteria from lesions, Edwardsiella tarda was selected by means of PCR. To obtain molecular acceptance, a pair of E. tarda special primer, forward primer ETa2-351 and reverse primer (Edwsp-780r) were reproduced. A number of 12 E. tarda DNA sample were identified by PCR. After molecular diagnosis, Persian sturgeon challenged with E. tarda for determination of pathogenesis. Challenge method was done by means of injection of different dilutes of E. tarda into dorsal muscle. Sampling of hematopoietic organs (kidney, spleen and liver) were carried out and located in Boin's fixator to perform pathology survey. Also, in order to survey of existence and effect of E. tarda, sampling of kidney for bacterial culture was done by molecular and biochemical methods. Results showed that the most lesions in all five species belonged to abdominal surface. Skin and scutes of this part were involved in comparison with other parts. Also, It was removed some samples from lesions to pathological survey. Microscopic observations showed some levels of destruction of epidermis layers, necrosis of dermis cells and destruction of muscular layer of skin. On the other hand, invasion of inflammatory cells and haemorrhagic in dermis were clear. Based on biochemical results, Aeromonas sobria, A cavia . A. hydrophila , Acinetobacter lowffii , A.baumanni , A.cakoaceticus, Pseudomonas putida , P fluorescens , P.aeruginosa , Serratia marcescens , Escherichia coli , Enterobacter ... Thesis Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications Gilan ENVELOPE(9.732,9.732,63.177,63.177) |
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Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
Persian |
topic |
Aquaculture |
spellingShingle |
Aquaculture Seyed Javad, Abolghasemi Study of bacterial agents on external lesions of cultured Sturgeons in Sngar zone of Gilan Province |
topic_facet |
Aquaculture |
description |
This study was carried out to isolate and determine bacterial agents in outer lesions of sturgeons in Shahid Dr. Beheshti sturgeon propagation and rearing center in Gilan province. Five species of sturgeons were studied from viewpoint of lesions. A number of 167 specimens of Beluga, 76 specimens of Persian sturgeon, 27 specimens of Russian sturgeon, 42 specimens of stellate and finally 23 specimens of ship had bacterial lesions in different outer parts of their bodies. After sampling and purification, bacterial cultures and biochemical tests were done. After the isolation of bacteria from lesions, Edwardsiella tarda was selected by means of PCR. To obtain molecular acceptance, a pair of E. tarda special primer, forward primer ETa2-351 and reverse primer (Edwsp-780r) were reproduced. A number of 12 E. tarda DNA sample were identified by PCR. After molecular diagnosis, Persian sturgeon challenged with E. tarda for determination of pathogenesis. Challenge method was done by means of injection of different dilutes of E. tarda into dorsal muscle. Sampling of hematopoietic organs (kidney, spleen and liver) were carried out and located in Boin's fixator to perform pathology survey. Also, in order to survey of existence and effect of E. tarda, sampling of kidney for bacterial culture was done by molecular and biochemical methods. Results showed that the most lesions in all five species belonged to abdominal surface. Skin and scutes of this part were involved in comparison with other parts. Also, It was removed some samples from lesions to pathological survey. Microscopic observations showed some levels of destruction of epidermis layers, necrosis of dermis cells and destruction of muscular layer of skin. On the other hand, invasion of inflammatory cells and haemorrhagic in dermis were clear. Based on biochemical results, Aeromonas sobria, A cavia . A. hydrophila , Acinetobacter lowffii , A.baumanni , A.cakoaceticus, Pseudomonas putida , P fluorescens , P.aeruginosa , Serratia marcescens , Escherichia coli , Enterobacter ... |
format |
Thesis |
author |
Seyed Javad, Abolghasemi |
author_facet |
Seyed Javad, Abolghasemi |
author_sort |
Seyed Javad, Abolghasemi |
title |
Study of bacterial agents on external lesions of cultured Sturgeons in Sngar zone of Gilan Province |
title_short |
Study of bacterial agents on external lesions of cultured Sturgeons in Sngar zone of Gilan Province |
title_full |
Study of bacterial agents on external lesions of cultured Sturgeons in Sngar zone of Gilan Province |
title_fullStr |
Study of bacterial agents on external lesions of cultured Sturgeons in Sngar zone of Gilan Province |
title_full_unstemmed |
Study of bacterial agents on external lesions of cultured Sturgeons in Sngar zone of Gilan Province |
title_sort |
study of bacterial agents on external lesions of cultured sturgeons in sngar zone of gilan province |
publisher |
Islamic Azad University, Science and Research Branch, Tehran, Aquatic Animal Health |
publishDate |
2009 |
url |
http://hdl.handle.net/1834/35774 |
long_lat |
ENVELOPE(9.732,9.732,63.177,63.177) |
geographic |
Gilan |
geographic_facet |
Gilan |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
http://aquaticcommons.org/id/eprint/20912 17408 2016-07-15 09:17:40 20912 Islamic Azad University, Science and Research Branch, Tehran |
op_relation |
http://www.srbiau.ac.ir/ http://hdl.handle.net/1834/35774 |
_version_ |
1766374800722231296 |