Microsatellite primers for red drum (Sciaenops ocellatus)

• Main Authors: Karlsson, Sten, Renshaw, Mark A., Rexroad III, Caird E., Gold, John R.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2008
• Subjects: Biology; Ecology; Fisheries; Red drum; Sciaenops ocellatus
• Online Access: http://hdl.handle.net/1834/25477

In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101 nuclear-encoded microsatellitesdesigned and developed from a genomic library for red drum (Sciaenops ocellatus). Details of the genomic library construction, the sequencing of positive clones, primer design, and PCR protocols may be found in Karlsson et al. (2008). The 101 microsatellites (GENBA NK Accession NumbersEU015882-EU015982) were amplified successfully and used to genotype 24 red drum obtained from Galveston Bay, Texas (Table 1). A total of 69 of the microsatellites had an uninterrupted (perfect) dinucleotide motif, and 30 had an imperfect dinucleotide motif; one microsatellite had animperfect tetranucleotide motif, and one had an imperfect and compound motif (Table 1 ). Sizes of the cloned alleles ranged from 84 to 252 base pairs. A ‘blast’ search of the GENBANK database indicated that all of the primers and the cloned alleles were unique (i.e., not duplicated).