The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique
The goal of this study was to find a sex-specific DNA sequences in the male and female genomes of beluga (Huso huso), by random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Of the 310 different primers was tested, 7 primers produced no band. The most likely possibility is...
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ftoceandocs:oai:aquadocs.org:1834/13019 2023-05-15T15:41:30+02:00 The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique Keyvan shokoo, S. Pourkazemi, M. Kalbasi, M.R. Iran Caspian Sea 2004 pp.149-162 http://hdl.handle.net/1834/13019 fa per http://isfj.areo.ir/ 1026-1354 http://hdl.handle.net/1834/13019 Beluga Huso huso Acipenseridae RAPD Sex marker DNA Male Female Samples Genome Journal Contribution Refereed 2004 ftoceandocs 2023-04-06T16:59:31Z The goal of this study was to find a sex-specific DNA sequences in the male and female genomes of beluga (Huso huso), by random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Of the 310 different primers was tested, 7 primers produced no band. The most likely possibility is that PCR conditions have not been suitable for these 7 primers. It is also possible that these RAPD primers did not find suitable binding position to generate fragments. A single band was produced by primer 295 (OPT -17) which was specific to the male DNA. Further analysis of individual male and female beluga demonstrated that this primer yeilded a product that were in fact variable and reproducible, but were the result of variation in some individual males and females and not male-specific. Therefore, of the 4146 RAPDs scored, none were tightly linked to a sex-determining gene. The results support the idea that sex chromosomes are either not present or they weakly differentiated in the genome of beluga. Published Book Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
Persian |
topic |
Beluga Huso huso Acipenseridae RAPD Sex marker DNA Male Female Samples Genome |
spellingShingle |
Beluga Huso huso Acipenseridae RAPD Sex marker DNA Male Female Samples Genome Keyvan shokoo, S. Pourkazemi, M. Kalbasi, M.R. The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
topic_facet |
Beluga Huso huso Acipenseridae RAPD Sex marker DNA Male Female Samples Genome |
description |
The goal of this study was to find a sex-specific DNA sequences in the male and female genomes of beluga (Huso huso), by random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Of the 310 different primers was tested, 7 primers produced no band. The most likely possibility is that PCR conditions have not been suitable for these 7 primers. It is also possible that these RAPD primers did not find suitable binding position to generate fragments. A single band was produced by primer 295 (OPT -17) which was specific to the male DNA. Further analysis of individual male and female beluga demonstrated that this primer yeilded a product that were in fact variable and reproducible, but were the result of variation in some individual males and females and not male-specific. Therefore, of the 4146 RAPDs scored, none were tightly linked to a sex-determining gene. The results support the idea that sex chromosomes are either not present or they weakly differentiated in the genome of beluga. Published |
format |
Book |
author |
Keyvan shokoo, S. Pourkazemi, M. Kalbasi, M.R. |
author_facet |
Keyvan shokoo, S. Pourkazemi, M. Kalbasi, M.R. |
author_sort |
Keyvan shokoo, S. |
title |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_short |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_full |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_fullStr |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_full_unstemmed |
The possibility of sex identification in beluga (Huso huso) by using PCR-RAPD technique |
title_sort |
possibility of sex identification in beluga (huso huso) by using pcr-rapd technique |
publishDate |
2004 |
url |
http://hdl.handle.net/1834/13019 |
op_coverage |
Iran Caspian Sea |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
http://isfj.areo.ir/ 1026-1354 http://hdl.handle.net/1834/13019 |
_version_ |
1766374394075021312 |