The determination of LC50 of diazinon and its sub-lethal effect on haematological indices of beluga (Huso huso)

The acute toxicity and effects of diazinon on some hematological indices of Beluga (Huso huso) juveniles weighting 4.3±0.12 grams was assessed following the O.E.C.D. direction in a static temperature in the range 20.27±2.05 ֯C. The 96h LC50 value of diazinon for beluga juveniles was 5.821. Also, the...

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Main Authors: Shamloofar, M., Kamali, A., Piri, M., Yaghmaie, F., Makhtoomi, N.
Format: Book
Language:Persian
Published: 2007
Subjects:
Online Access:http://hdl.handle.net/1834/12881
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record_format openpolar
spelling ftoceandocs:oai:aquadocs.org:1834/12881 2023-05-15T15:41:26+02:00 The determination of LC50 of diazinon and its sub-lethal effect on haematological indices of beluga (Huso huso) Shamloofar, M. Kamali, A. Piri, M. Yaghmaie, F. Makhtoomi, N. Iran 2007 pp.69-78 http://hdl.handle.net/1834/12881 fa per http://isfj.areo.ir/ 1026-1354 http://hdl.handle.net/1834/12881 Experimental LC50 sub-lethal Beluga Huso huso Diazinon Hematological indices Toxicity Temperature Symptom Syndrome specimens hemoglobin hematocrit lymphocyte Specimens Hemoglobin Hematocrit Lymphocyte Journal Contribution Refereed 2007 ftoceandocs 2023-04-06T16:59:28Z The acute toxicity and effects of diazinon on some hematological indices of Beluga (Huso huso) juveniles weighting 4.3±0.12 grams was assessed following the O.E.C.D. direction in a static temperature in the range 20.27±2.05 ֯C. The 96h LC50 value of diazinon for beluga juveniles was 5.821. Also, the maximum allowable concentration of diazinon in natural waters for beluga was determined to be 0.5821mg.l-1. Based on the toxicity table of insecticides, diazinon was listed as toxic for beluga. The clinical symptoms that were observed in this study consisted of lordosis and neural paralytic syndrome in fish exposed to the pesticide. Some abnormal reactions such as losing the balance when swimming and swimming in a half circle; expressive pigmentation mainly on the dorsal part were seen in the juveniles. Examination of hematological indices was performed on control and experimental specimens of beluga weighting 16.08 grams on average that were treated with 96h exposure to diazinon in a concentration lower than LC50 96h. The experimental group of beluga showed significantly lower value (P<0.05) of ,erythrocyte (RBC) and leukocyte count, hemoglobin content (Hb), and hematocrit (PCV), MCV, MCH and relative lymphocyte and eosinophil counts compared to the control group .1n comparison, the relative heterophil count in the juveniles of the experimental group was significantly higher than the control group. By observing a decrease in the amount of leukocyte profile specially lymphocytes which are important in non-specific immunity of the fish, it can be said that diazinon may cause a decrease in the non-specific immunity of beluga. Published Book Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications
institution Open Polar
collection IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications
op_collection_id ftoceandocs
language Persian
topic Experimental
LC50
sub-lethal
Beluga
Huso huso
Diazinon
Hematological indices
Toxicity
Temperature
Symptom
Syndrome
specimens hemoglobin hematocrit lymphocyte
Specimens
Hemoglobin
Hematocrit
Lymphocyte
spellingShingle Experimental
LC50
sub-lethal
Beluga
Huso huso
Diazinon
Hematological indices
Toxicity
Temperature
Symptom
Syndrome
specimens hemoglobin hematocrit lymphocyte
Specimens
Hemoglobin
Hematocrit
Lymphocyte
Shamloofar, M.
Kamali, A.
Piri, M.
Yaghmaie, F.
Makhtoomi, N.
The determination of LC50 of diazinon and its sub-lethal effect on haematological indices of beluga (Huso huso)
topic_facet Experimental
LC50
sub-lethal
Beluga
Huso huso
Diazinon
Hematological indices
Toxicity
Temperature
Symptom
Syndrome
specimens hemoglobin hematocrit lymphocyte
Specimens
Hemoglobin
Hematocrit
Lymphocyte
description The acute toxicity and effects of diazinon on some hematological indices of Beluga (Huso huso) juveniles weighting 4.3±0.12 grams was assessed following the O.E.C.D. direction in a static temperature in the range 20.27±2.05 ֯C. The 96h LC50 value of diazinon for beluga juveniles was 5.821. Also, the maximum allowable concentration of diazinon in natural waters for beluga was determined to be 0.5821mg.l-1. Based on the toxicity table of insecticides, diazinon was listed as toxic for beluga. The clinical symptoms that were observed in this study consisted of lordosis and neural paralytic syndrome in fish exposed to the pesticide. Some abnormal reactions such as losing the balance when swimming and swimming in a half circle; expressive pigmentation mainly on the dorsal part were seen in the juveniles. Examination of hematological indices was performed on control and experimental specimens of beluga weighting 16.08 grams on average that were treated with 96h exposure to diazinon in a concentration lower than LC50 96h. The experimental group of beluga showed significantly lower value (P<0.05) of ,erythrocyte (RBC) and leukocyte count, hemoglobin content (Hb), and hematocrit (PCV), MCV, MCH and relative lymphocyte and eosinophil counts compared to the control group .1n comparison, the relative heterophil count in the juveniles of the experimental group was significantly higher than the control group. By observing a decrease in the amount of leukocyte profile specially lymphocytes which are important in non-specific immunity of the fish, it can be said that diazinon may cause a decrease in the non-specific immunity of beluga. Published
format Book
author Shamloofar, M.
Kamali, A.
Piri, M.
Yaghmaie, F.
Makhtoomi, N.
author_facet Shamloofar, M.
Kamali, A.
Piri, M.
Yaghmaie, F.
Makhtoomi, N.
author_sort Shamloofar, M.
title The determination of LC50 of diazinon and its sub-lethal effect on haematological indices of beluga (Huso huso)
title_short The determination of LC50 of diazinon and its sub-lethal effect on haematological indices of beluga (Huso huso)
title_full The determination of LC50 of diazinon and its sub-lethal effect on haematological indices of beluga (Huso huso)
title_fullStr The determination of LC50 of diazinon and its sub-lethal effect on haematological indices of beluga (Huso huso)
title_full_unstemmed The determination of LC50 of diazinon and its sub-lethal effect on haematological indices of beluga (Huso huso)
title_sort determination of lc50 of diazinon and its sub-lethal effect on haematological indices of beluga (huso huso)
publishDate 2007
url http://hdl.handle.net/1834/12881
op_coverage Iran
genre Beluga
Beluga*
genre_facet Beluga
Beluga*
op_relation http://isfj.areo.ir/
1026-1354
http://hdl.handle.net/1834/12881
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