Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish
The aim of the present study was to detect polymorphism in Insulin like growth factor-I (IGF-I) gene of beluga (Huso huso) fish using PCR-SSCP technique and also investigation of its association with growth traits (condition factor, body length and weight). A total of 150 specimens of beluga were ra...
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ftoceandocs:oai:aquadocs.org:1834/12272 2023-05-15T15:41:33+02:00 Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish Zeinaddini Meymand, Z. Yeganeh, S. Rahimi-Mianji, Gh. Farhadi, A. Iran 2017 pp.1257-1266 http://hdl.handle.net/1834/12272 en eng 1562-2916 http://hdl.handle.net/1834/12272 Huso huso Polymorphism Beluga 3'-UTR 5'-UTR PCR-SSCP Growth traits Fish Journal Contribution Refereed 2017 ftoceandocs 2023-04-06T16:59:20Z The aim of the present study was to detect polymorphism in Insulin like growth factor-I (IGF-I) gene of beluga (Huso huso) fish using PCR-SSCP technique and also investigation of its association with growth traits (condition factor, body length and weight). A total of 150 specimens of beluga were randomly selected and DNA was isolated from caudal fin using modified salting out method. Then two fragments of 171 and 362 bp from 5'-UTR and 3'-UTR regions of IGF-I gene were amplified, respectively. Genotyping of individuals by SSCP technique showed five banding patterns of A, B, C, D and E for 5'-UTR region with the frequencies of 29.2, 0.76, 16.92, 51.53 and 10% respectively in one year-old and three banding patterns of A, C and D with the frequency of 45, 10 and 45% for two year-old fish. Also three banding patterns (A, B and C) were seen for 3'-UTR region with the frequency of 62.3, 27.69 and 10.76% in one-year-old and 20, 60 and 20% in two year-old fish. The A banding pattern in 3'-UTR and D banding pattern in 5'-UTR sites were the most frequent pattern in the studied beluga population. The association analysis using SAS statistical software indicated no significant association between observed banding patterns and growth traits (body length, weight, and condition factor) in beluga. Considering the important role of IGF-I as a probable candidate gene affecting growth related traits, these marker sites should be studied more in larger sample sizes and also in other regions of the gene. Published Book Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
English |
topic |
Huso huso Polymorphism Beluga 3'-UTR 5'-UTR PCR-SSCP Growth traits Fish |
spellingShingle |
Huso huso Polymorphism Beluga 3'-UTR 5'-UTR PCR-SSCP Growth traits Fish Zeinaddini Meymand, Z. Yeganeh, S. Rahimi-Mianji, Gh. Farhadi, A. Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish |
topic_facet |
Huso huso Polymorphism Beluga 3'-UTR 5'-UTR PCR-SSCP Growth traits Fish |
description |
The aim of the present study was to detect polymorphism in Insulin like growth factor-I (IGF-I) gene of beluga (Huso huso) fish using PCR-SSCP technique and also investigation of its association with growth traits (condition factor, body length and weight). A total of 150 specimens of beluga were randomly selected and DNA was isolated from caudal fin using modified salting out method. Then two fragments of 171 and 362 bp from 5'-UTR and 3'-UTR regions of IGF-I gene were amplified, respectively. Genotyping of individuals by SSCP technique showed five banding patterns of A, B, C, D and E for 5'-UTR region with the frequencies of 29.2, 0.76, 16.92, 51.53 and 10% respectively in one year-old and three banding patterns of A, C and D with the frequency of 45, 10 and 45% for two year-old fish. Also three banding patterns (A, B and C) were seen for 3'-UTR region with the frequency of 62.3, 27.69 and 10.76% in one-year-old and 20, 60 and 20% in two year-old fish. The A banding pattern in 3'-UTR and D banding pattern in 5'-UTR sites were the most frequent pattern in the studied beluga population. The association analysis using SAS statistical software indicated no significant association between observed banding patterns and growth traits (body length, weight, and condition factor) in beluga. Considering the important role of IGF-I as a probable candidate gene affecting growth related traits, these marker sites should be studied more in larger sample sizes and also in other regions of the gene. Published |
format |
Book |
author |
Zeinaddini Meymand, Z. Yeganeh, S. Rahimi-Mianji, Gh. Farhadi, A. |
author_facet |
Zeinaddini Meymand, Z. Yeganeh, S. Rahimi-Mianji, Gh. Farhadi, A. |
author_sort |
Zeinaddini Meymand, Z. |
title |
Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish |
title_short |
Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish |
title_full |
Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish |
title_fullStr |
Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish |
title_full_unstemmed |
Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish |
title_sort |
insulin-like growth factor i gene polymorphism associated with growth traits in beluga (huso huso) fish |
publishDate |
2017 |
url |
http://hdl.handle.net/1834/12272 |
op_coverage |
Iran |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
1562-2916 http://hdl.handle.net/1834/12272 |
_version_ |
1766374444178079744 |