Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey
Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases in farmed rainbow trout, in Turkey, which is often associated...
Main Authors: | , , , , |
---|---|
Format: | Book |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | http://hdl.handle.net/1834/12226 |
id |
ftoceandocs:oai:aquadocs.org:1834/12226 |
---|---|
record_format |
openpolar |
spelling |
ftoceandocs:oai:aquadocs.org:1834/12226 2023-05-15T18:15:44+02:00 Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey Albayrak, H. Isidan, H. Kalayci, G. Ozan, E. Vakharia, V.N. Turkey 2018 pp.67-73 http://hdl.handle.net/1834/12226 en eng 1562-2916 http://hdl.handle.net/1834/12226 Novirhabdovirus Oncorhynchus mykiss Scophthalmus maximus Genotype Rainbow trout Turbot VHSV Genetic Gene Journal Contribution Refereed 2018 ftoceandocs 2023-04-06T16:59:19Z Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases in farmed rainbow trout, in Turkey, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout (Oncorhynchus mykiss) samples were collected during an outbreak of VHSV in a rainbow trout fish farm in Bolu Province of Turkey in 2006. In addition, two VHSV strains were isolated from wild turbot (Scophthalmus maximus) in Trabzon Province of the Black Sea region of Turkey during a field survey. We have sequenced the full-length glycoprotein (G) gene of three VHSV isolates and compared them with 25 previously published gene sequences. Based on a complete gene nucleotide sequence, Turkish VHSV isolates were classified into class Ie of genotype I, which is closely related to GE-1.2 isolate (97.1-97.5% nucleotide identity and 98.2-98.4% amino acid identity) found in Georgia more than 30 years ago. These isolates could be an indigenous type of VHSV distributed in the Black Sea. On the other hand, Turkish isolates have 97.5-97.6% nucleotide identity and 98.8-99% amino acid identity with Finnish, Danish, and Norwegian isolates which are classified under Ib and Id. These results suggest that Turkish VHSV isolates may have orginated from Europe and co-circulated with indigenous strains which can threaten the aquaculture industry in Turkey. Published Book Scophthalmus maximus Turbot IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
English |
topic |
Novirhabdovirus Oncorhynchus mykiss Scophthalmus maximus Genotype Rainbow trout Turbot VHSV Genetic Gene |
spellingShingle |
Novirhabdovirus Oncorhynchus mykiss Scophthalmus maximus Genotype Rainbow trout Turbot VHSV Genetic Gene Albayrak, H. Isidan, H. Kalayci, G. Ozan, E. Vakharia, V.N. Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
topic_facet |
Novirhabdovirus Oncorhynchus mykiss Scophthalmus maximus Genotype Rainbow trout Turbot VHSV Genetic Gene |
description |
Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases in farmed rainbow trout, in Turkey, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout (Oncorhynchus mykiss) samples were collected during an outbreak of VHSV in a rainbow trout fish farm in Bolu Province of Turkey in 2006. In addition, two VHSV strains were isolated from wild turbot (Scophthalmus maximus) in Trabzon Province of the Black Sea region of Turkey during a field survey. We have sequenced the full-length glycoprotein (G) gene of three VHSV isolates and compared them with 25 previously published gene sequences. Based on a complete gene nucleotide sequence, Turkish VHSV isolates were classified into class Ie of genotype I, which is closely related to GE-1.2 isolate (97.1-97.5% nucleotide identity and 98.2-98.4% amino acid identity) found in Georgia more than 30 years ago. These isolates could be an indigenous type of VHSV distributed in the Black Sea. On the other hand, Turkish isolates have 97.5-97.6% nucleotide identity and 98.8-99% amino acid identity with Finnish, Danish, and Norwegian isolates which are classified under Ib and Id. These results suggest that Turkish VHSV isolates may have orginated from Europe and co-circulated with indigenous strains which can threaten the aquaculture industry in Turkey. Published |
format |
Book |
author |
Albayrak, H. Isidan, H. Kalayci, G. Ozan, E. Vakharia, V.N. |
author_facet |
Albayrak, H. Isidan, H. Kalayci, G. Ozan, E. Vakharia, V.N. |
author_sort |
Albayrak, H. |
title |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_short |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_full |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_fullStr |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_full_unstemmed |
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey |
title_sort |
genetic analysis of the complete g gene of viral hemorrhagic septicemia virus (vhsv) genotype ie isolates from turkey |
publishDate |
2018 |
url |
http://hdl.handle.net/1834/12226 |
op_coverage |
Turkey |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
1562-2916 http://hdl.handle.net/1834/12226 |
_version_ |
1766188942179172352 |