Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique
In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, ge...
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ftoceandocs:oai:aquadocs.org:1834/11510 2023-05-15T15:41:43+02:00 Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. Iran Rasht 2012 pp.415-423 http://hdl.handle.net/1834/11510 en eng 1562-2916 http://hdl.handle.net/1834/11510 Huso huso Acipenser ruthenus AFLP Hybrid identification Bester Beluga Biochemistry Identification Journal Contribution Refereed 2012 ftoceandocs 2023-04-06T16:59:14Z In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster analyses (UPGMA) allow a correct identification. We used 8 primer combinations (Eco+3, Mse+4) and a total of 250 bands (size range 40-1000 bp) were generated. Primer combinations of (E-AAT, M-CGAT) and (E-AAG, M-CGAT) produced diagnostic bands in hybrids and parental species. Moreover, the results of genetic identification showed that Bester hybrids are more similar to beluga (Huso huso) (0.68) in comparison with sterlet (Acipenser ruthenus) (0.45).The results suggested that this technique could be suitable for precise identification of species and inter-generic hybrids like bester. Published Book Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
English |
topic |
Huso huso Acipenser ruthenus AFLP Hybrid identification Bester Beluga Biochemistry Identification |
spellingShingle |
Huso huso Acipenser ruthenus AFLP Hybrid identification Bester Beluga Biochemistry Identification Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
topic_facet |
Huso huso Acipenser ruthenus AFLP Hybrid identification Bester Beluga Biochemistry Identification |
description |
In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster analyses (UPGMA) allow a correct identification. We used 8 primer combinations (Eco+3, Mse+4) and a total of 250 bands (size range 40-1000 bp) were generated. Primer combinations of (E-AAT, M-CGAT) and (E-AAG, M-CGAT) produced diagnostic bands in hybrids and parental species. Moreover, the results of genetic identification showed that Bester hybrids are more similar to beluga (Huso huso) (0.68) in comparison with sterlet (Acipenser ruthenus) (0.45).The results suggested that this technique could be suitable for precise identification of species and inter-generic hybrids like bester. Published |
format |
Book |
author |
Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. |
author_facet |
Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. |
author_sort |
Yarmohammadi, M. |
title |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_short |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_full |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_fullStr |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_full_unstemmed |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_sort |
identification of bester hybrids (female huso huso linnaeus, 1758 and male sterlet acipenser ruthenus linnaeus, 1758) using aflp molecular technique |
publishDate |
2012 |
url |
http://hdl.handle.net/1834/11510 |
op_coverage |
Iran Rasht |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
1562-2916 http://hdl.handle.net/1834/11510 |
_version_ |
1766374617824362496 |