Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso)
The influence of dissolved oxygen concentration on gill histopathology of great sturgeon (Huso huso) was evaluated in two weight classes (initial weight 280.9±49.2 g and 1217.9±138.1 g respectively). Oxygen treatments included hypoxia (2-3 mg/l), normoxia (5-6 mg/l) and hyperoxia (9-10 mg/l). The fi...
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ftoceandocs:oai:aquadocs.org:1834/10481 2023-05-15T15:41:53+02:00 Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) Bagherzadeh Lakani, F. Sattari, M. Sharifpour, I. Kazemi, R. Iran 2013 pp.77-84 http://hdl.handle.net/1834/10481 en eng 1735-3033 http://hdl.handle.net/1834/10481 Huso huso Normoxia Hypoxia Gill Histopathology Weight Hyperoxia Fish Sturgeon Journal Contribution Refereed 2013 ftoceandocs 2023-04-06T17:00:17Z The influence of dissolved oxygen concentration on gill histopathology of great sturgeon (Huso huso) was evaluated in two weight classes (initial weight 280.9±49.2 g and 1217.9±138.1 g respectively). Oxygen treatments included hypoxia (2-3 mg/l), normoxia (5-6 mg/l) and hyperoxia (9-10 mg/l). The fish were acclimated to experimental tanks for one week then randomly distributed into 9 tanks in each of the initial weight classes (3 and 6 fish per tank in higher and lower initial weight classes respectively) for 8 weeks. In order to find the histopathological changes, gill samples were collected, dehydrated through ethanol series, embedded in paraffin , sectioned at 7 µm thickness using a Leitz microtome and stained with H & E. No mortality was observed over the 8 weeks of the experimental period. There were significant differences in weight and feed intake between treatments in the both weight classes (P<0.05). Fork length showed significant differences in lower initial weight class (P<0.05). The main histopathological changes were observed in gills including: Hyperplasia, loss of secondary lamellae, hemorrhage and congestion in primary and secondary lamellae, lamellar fusion, epithelial lifting in secondary lamellae, clubbing of secondary lamellae, telangiectases, increase in melanin pigments and numerous vacuoles in primary and secondary lamellae (in hyperoxia treatment). All these lesions may reduce gill functional surface of gaseous exchange, impairing respiratory function. Published Book Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
English |
topic |
Huso huso Normoxia Hypoxia Gill Histopathology Weight Hyperoxia Fish Sturgeon |
spellingShingle |
Huso huso Normoxia Hypoxia Gill Histopathology Weight Hyperoxia Fish Sturgeon Bagherzadeh Lakani, F. Sattari, M. Sharifpour, I. Kazemi, R. Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
topic_facet |
Huso huso Normoxia Hypoxia Gill Histopathology Weight Hyperoxia Fish Sturgeon |
description |
The influence of dissolved oxygen concentration on gill histopathology of great sturgeon (Huso huso) was evaluated in two weight classes (initial weight 280.9±49.2 g and 1217.9±138.1 g respectively). Oxygen treatments included hypoxia (2-3 mg/l), normoxia (5-6 mg/l) and hyperoxia (9-10 mg/l). The fish were acclimated to experimental tanks for one week then randomly distributed into 9 tanks in each of the initial weight classes (3 and 6 fish per tank in higher and lower initial weight classes respectively) for 8 weeks. In order to find the histopathological changes, gill samples were collected, dehydrated through ethanol series, embedded in paraffin , sectioned at 7 µm thickness using a Leitz microtome and stained with H & E. No mortality was observed over the 8 weeks of the experimental period. There were significant differences in weight and feed intake between treatments in the both weight classes (P<0.05). Fork length showed significant differences in lower initial weight class (P<0.05). The main histopathological changes were observed in gills including: Hyperplasia, loss of secondary lamellae, hemorrhage and congestion in primary and secondary lamellae, lamellar fusion, epithelial lifting in secondary lamellae, clubbing of secondary lamellae, telangiectases, increase in melanin pigments and numerous vacuoles in primary and secondary lamellae (in hyperoxia treatment). All these lesions may reduce gill functional surface of gaseous exchange, impairing respiratory function. Published |
format |
Book |
author |
Bagherzadeh Lakani, F. Sattari, M. Sharifpour, I. Kazemi, R. |
author_facet |
Bagherzadeh Lakani, F. Sattari, M. Sharifpour, I. Kazemi, R. |
author_sort |
Bagherzadeh Lakani, F. |
title |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_short |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_full |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_fullStr |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_full_unstemmed |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_sort |
effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (huso huso) |
publishDate |
2013 |
url |
http://hdl.handle.net/1834/10481 |
op_coverage |
Iran |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
1735-3033 http://hdl.handle.net/1834/10481 |
_version_ |
1766374766994784256 |