Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA)
In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive proced...
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ftoceandocs:oai:aquadocs.org:1834/10467 2023-05-15T15:41:40+02:00 Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. Iran 2014 pp.185-195 http://hdl.handle.net/1834/10467 en eng 1735-3033 http://hdl.handle.net/1834/10467 Huso huso Bulked segregant analysis (BSA) Beluga Genome Sex Sex markers Genetics Journal Contribution Refereed 2014 ftoceandocs 2023-04-06T17:00:19Z In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we employed Bulked Segregant Analysis (BSA) methodology to search for DNA markers associated with the sex of the beluga sturgeon (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 101 decamer primers associated with the sex-specific sequences in non-sturgeon species was used for targeted screening of the bulks, resulting in 2846 bands that all of them were present in both sexes. Our results showed that sex chromosomes are weakly differentiated in the sturgeon genome and comprised sequences not complementary to the sex-specific primers in non-sturgeon species. Published Book Beluga Beluga* IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
institution |
Open Polar |
collection |
IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications |
op_collection_id |
ftoceandocs |
language |
English |
topic |
Huso huso Bulked segregant analysis (BSA) Beluga Genome Sex Sex markers Genetics |
spellingShingle |
Huso huso Bulked segregant analysis (BSA) Beluga Genome Sex Sex markers Genetics Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
topic_facet |
Huso huso Bulked segregant analysis (BSA) Beluga Genome Sex Sex markers Genetics |
description |
In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we employed Bulked Segregant Analysis (BSA) methodology to search for DNA markers associated with the sex of the beluga sturgeon (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 101 decamer primers associated with the sex-specific sequences in non-sturgeon species was used for targeted screening of the bulks, resulting in 2846 bands that all of them were present in both sexes. Our results showed that sex chromosomes are weakly differentiated in the sturgeon genome and comprised sequences not complementary to the sex-specific primers in non-sturgeon species. Published |
format |
Book |
author |
Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. |
author_facet |
Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. |
author_sort |
Khodaparast, M. |
title |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_short |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_full |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_fullStr |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_full_unstemmed |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_sort |
searching the genome of beluga (huso huso) for sex markers based on targeted bulked segregant analysis (bsa) |
publishDate |
2014 |
url |
http://hdl.handle.net/1834/10467 |
op_coverage |
Iran |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
1735-3033 http://hdl.handle.net/1834/10467 |
_version_ |
1766374570198040576 |