Upotreba molekularnih biljega u identifikaciji različitih vrsta kamenica u Jadranu

• Main Author: Vondrak, Tihana
• Other Authors: Mravinac, Brankica, Matulić, Maja
• Format: Master Thesis
• Language: Croatian
• Published: Sveučilište u Zagrebu. Prirodoslovno-matematički fakultet. Biološki odsjek. 2017
• Subjects: Crassostrea gigas; Ostrea edulis; 16S rDNA; COI; ITS1; ITS2; 5S rDNA; PRIRODNE ZNANOSTI. Biologija; NATURAL SCIENCES. Biology
• Online Access: https://zir.nsk.hr/islandora/object/pmf:4522; https://urn.nsk.hr/urn:nbn:hr:217:444893; https://repozitorij.unizg.hr/islandora/object/pmf:4522; https://repozitorij.unizg.hr/islandora/object/pmf:4522/datastream/PDF

Školjkaš Crassostrea gigas (Thunberg 1793) prirodno je rasprostranjen na obalama Japana te jugoistočne Azije. Ova vrsta uvedena je na područje Europe 1960-tih godina kako bi zamijenila izlovljene populacije autohtone vrste Ostrea edulis (Linnaeus 1758). Vrsta O. edulis je gospodarski vrlo važan školjkaš široko prisutan u Jadranu, dok je vrsta C. gigas u hrvatski dio Jadrana unesena relativno nedavno. Isključivo morfološko razlikovanje vrsta O. edulis i C. gigas može biti nepouzdano. Stoga su u ovom radu korišteni mitohondrijski biljezi 16S rDNA i COI kako bi se potvrdila prisutnost alohtone vrste C. gigas u području sjevernog Jadrana kao i njezino širenje u područje srednjeg Jadrana. Ovim radom dokazano je također da se nuklearni biljezi 5S rDNA, ITS1 i ITS2 mogu koristiti u svrhu uspješnog razlikovanja jedinki vrsta C. gigas i O. edulis. The clam Crassostrea gigas (Thunberg 1793) is a native species on the coast of Japan and Southeast Asia. C. gigas was introduced into European coastal waters in the 1960’s in an attempt to replace the overexploited populations of the native clam Ostrea edulis (Linnaeus 1758). The species O. edulis is economically very important and is widely present on the coast of the Adriatic sea, while the species C. gigas has been recently introduced onto the Croatian coast of the Adriatic. Discrimination between species O. edulis and C. gigas based only on morphology can be unreliable. Therefore the mitochondrial markers 16S rDNA and COI were used to confirm the presence of the invasive species C. gigas in the northern Adriatic as well as its propagation to the central Adriatic. Furthermore this thesis proved that nuclear markers 5S rDNA, ITS1 and ITS2 can be used for successful differentiation between the species C. gigas and O. edulis.