Priprava imidazolijevih ionskih tekućina i njihova primjena u biotransformacijama

• Main Author: Radoš, Ivan
• Other Authors: Vorkapić-Furač, Jasna
• Format: Master Thesis
• Language: Croatian
• Published: Sveučilište u Zagrebu. Prehrambeno-biotehnološki fakultet. Zavod za biokemijsko inženjerstvo. Laboratorij za biologiju i genetiku mikroorganizama. 2011
• Subjects: imidazolijeve ionske tekućine; izoamil-acetat; mikroreaktorski sustav; mikromiješalo; Novozym 435; imidazolium ionic liquids; isoamyl acetate; micro mixer; micro reactor system; BIOTEHNIČKE ZNANOSTI. Biotehnologija; BIOTECHNICAL SCIENCES. Biotechnology; Antarc*; Antarctica
• Online Access: https://zir.nsk.hr/islandora/object/pbf:1390; https://urn.nsk.hr/urn:nbn:hr:159:261004; https://repozitorij.unizg.hr/islandora/object/pbf:1390; https://repozitorij.unizg.hr/islandora/object/pbf:1390/datastream/PDF

Cilj ovog rada bila je sinteza imidazolijevih ionskih tekućina te njihovo ispitivanje kao novih medija za biotransformacije. Ionske tekućine pripravljene su reakcijama kvaternizacije 1,2-dimetilimidazola s 1-brompentanom, 1-bromheksanom i 1-bromheptanom te reakcijama izmjene aniona s litijevim bis[(trifluormetil)sulfonil]imidom. Procesi sinteze provedeni su šaržno, klasičnim postupcima organske sinteze te kontinuirano u mikroreaktorskom sustavu s mikromješačem. Novosintetizirane ionske tekućine karakterizirane su 1H- i 13C-NMR-om. Također, ionske tekućine ispitane su kao novi mediji u reakciji sinteze izoamil-acetata s imobiliziranom lipazom B izoliranom iz kvasca Candida antarctica (Novozym 435), a za usporedbu, reakcija je provedena u komercijalnoj ionskoj tekućini [BMIm][Tf2N]. The aim of this study was the synthesis of imidazolium ionic liquids and their testing as new media for biotransformations. Ionic liquids were prepared by quaternization of 1,2-dimethylimidazole with 1-bromopentane, 1-bromohexane and 1-bromoheptane and anion exchange reactions with lithium bis[(trifluoromethyl)sulfonyl]imide. The syntheses were carried out by batch conventional organic synthesis methods, and continuously in a micro reactor system with micro mixer. The novel ionic liquids were characterized by 1H-and 13CNMR spectra. Also, ionic liquids have been examined as new media for the isoamyl acetate synthesis with immobilized lipase B isolated from Candida antarctica (Novozym 435). For the comparison the reaction in commercial ionic liquid [BMIm][Tf2N] was carried out.