Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data
Journal article This study set out to investigate whether differential expression of genes for acute phase proteins in rainbow trout (Oncorhynchus mykiss) could be induced by confinement stress, a non-invasive method of activating the neuroendocrine stress response. In addition, a second objective w...
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ftnuigalway:oai:https://researchrepository.universityofgalway.ie:10379/4671 2024-09-30T14:42:10+00:00 Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data Talbot, Anita T. Smith, Terry Cairns, Michael T. |~| 2014-10-23T13:10:57Z application/pdf http://hdl.handle.net/10379/4671 https://doi.org/10.13025/21620 en eng Elsevier Fish & Shellfish Immunology Talbot, AT,Pottinger, TG,Smith, TJ,Cairns, MT (2009) 'Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data'. Fish & Shellfish Immunology, 27 :309-317. 1050-4648 http://hdl.handle.net/10379/4671 https://doi.org/10.13025/21620 DOI 10.1016/j.fsi.2009.05.016 Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ Gene expression Confinement Stress Acute phase response Salmon salmo salar Suppression subtractive hybridization Carp cyprinus carpio Serum amyloid A Aeromonas salmonicida Handling stress Immune response Haptoglobin Infection Identification Article 2014 ftnuigalway https://doi.org/10.13025/21620 2024-09-17T14:44:29Z Journal article This study set out to investigate whether differential expression of genes for acute phase proteins in rainbow trout (Oncorhynchus mykiss) could be induced by confinement stress, a non-invasive method of activating the neuroendocrine stress response. In addition, a second objective was to assess the variation in gene expression between individual fish within the population of stressed fish in an attempt to identify APP genes having uniform and consistent changes in expression during stress. The liver was chosen for this investigation as it is the primary site of acute phase protein synthesis. Relative expression of the eight genes including transferrin, fibrinogen-like protein 2 (flp2), alpha-1-anti-proteinase-like protein (alpha 1-antiprot), leukocyte cell-derived chemotaxin 2 (LECT2), pentraxin, serum amyloid A (SAA), haptoglobin (Hp), and differentially regulated trout protein 1 (DRTP1) was analysed by quantitative real-time PCR (qPCR) over 5 experimental time points spanning the course of a week. The results showed that the expression of three genes, SAA, haptoglobin and DRTP1, were most altered as a result of exposure to confinement stress. A correlation was identified between the expression of haptoglobin and DRTP1. Gene expression analyses in individual fish found that the transcript levels of haptoglobin and DRTP1 genes varied much less between individuals than was the case for SAA. The increase of haptoglobin and DRTP1 gene expression and its uniformity in response to stress make these genes potential biomarkers for stress in trout. Enterprise Ireland - Basic Research Grant (SC/2003/0461) peer-reviewed Article in Journal/Newspaper Salmo salar National University of Ireland (NUI), Galway: ARAN |
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Open Polar |
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National University of Ireland (NUI), Galway: ARAN |
op_collection_id |
ftnuigalway |
language |
English |
topic |
Gene expression Confinement Stress Acute phase response Salmon salmo salar Suppression subtractive hybridization Carp cyprinus carpio Serum amyloid A Aeromonas salmonicida Handling stress Immune response Haptoglobin Infection Identification |
spellingShingle |
Gene expression Confinement Stress Acute phase response Salmon salmo salar Suppression subtractive hybridization Carp cyprinus carpio Serum amyloid A Aeromonas salmonicida Handling stress Immune response Haptoglobin Infection Identification Talbot, Anita T. Smith, Terry Cairns, Michael T. Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data |
topic_facet |
Gene expression Confinement Stress Acute phase response Salmon salmo salar Suppression subtractive hybridization Carp cyprinus carpio Serum amyloid A Aeromonas salmonicida Handling stress Immune response Haptoglobin Infection Identification |
description |
Journal article This study set out to investigate whether differential expression of genes for acute phase proteins in rainbow trout (Oncorhynchus mykiss) could be induced by confinement stress, a non-invasive method of activating the neuroendocrine stress response. In addition, a second objective was to assess the variation in gene expression between individual fish within the population of stressed fish in an attempt to identify APP genes having uniform and consistent changes in expression during stress. The liver was chosen for this investigation as it is the primary site of acute phase protein synthesis. Relative expression of the eight genes including transferrin, fibrinogen-like protein 2 (flp2), alpha-1-anti-proteinase-like protein (alpha 1-antiprot), leukocyte cell-derived chemotaxin 2 (LECT2), pentraxin, serum amyloid A (SAA), haptoglobin (Hp), and differentially regulated trout protein 1 (DRTP1) was analysed by quantitative real-time PCR (qPCR) over 5 experimental time points spanning the course of a week. The results showed that the expression of three genes, SAA, haptoglobin and DRTP1, were most altered as a result of exposure to confinement stress. A correlation was identified between the expression of haptoglobin and DRTP1. Gene expression analyses in individual fish found that the transcript levels of haptoglobin and DRTP1 genes varied much less between individuals than was the case for SAA. The increase of haptoglobin and DRTP1 gene expression and its uniformity in response to stress make these genes potential biomarkers for stress in trout. Enterprise Ireland - Basic Research Grant (SC/2003/0461) peer-reviewed |
author2 |
|~| |
format |
Article in Journal/Newspaper |
author |
Talbot, Anita T. Smith, Terry Cairns, Michael T. |
author_facet |
Talbot, Anita T. Smith, Terry Cairns, Michael T. |
author_sort |
Talbot, Anita T. |
title |
Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data |
title_short |
Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data |
title_full |
Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data |
title_fullStr |
Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data |
title_full_unstemmed |
Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data |
title_sort |
acute phase gene expression in rainbow trout (oncorhynchus mykiss) after exposure to a confinement stressor: a comparison of pooled and individual data |
publisher |
Elsevier |
publishDate |
2014 |
url |
http://hdl.handle.net/10379/4671 https://doi.org/10.13025/21620 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
Fish & Shellfish Immunology Talbot, AT,Pottinger, TG,Smith, TJ,Cairns, MT (2009) 'Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data'. Fish & Shellfish Immunology, 27 :309-317. 1050-4648 http://hdl.handle.net/10379/4671 https://doi.org/10.13025/21620 DOI 10.1016/j.fsi.2009.05.016 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.13025/21620 |
_version_ |
1811644390743998464 |