Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland
This study investigated the genotypes and sub-groups of infectious pancreatic necrosis virus (IPNV) present in farmed and wild salmonid fish in Ireland. An 1100-bp portion of the VP2 region of segment A from each of 55 IPNV isolates collected over 2003-2007 was amplified by reverse-transcription-pol...
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ftnuigalway:oai:https://researchrepository.universityofgalway.ie:10379/13720 2024-09-30T14:32:31+00:00 Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland Ruane, N M McCarthy, L J Swords, D Henshilwood, K 2009-12-01 http://hdl.handle.net/10379/13720 https://doi.org/10.13025/28655 https://doi.org/10.1111/j.1365-2761.2009.01080.x unknown Wiley-Blackwell Journal of Fish Diseases Ruane, N M; McCarthy, L J; Swords, D; Henshilwood, K (2009). Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland. Journal of Fish Diseases 32 (12), 979-987 0140-7775,1365-2761 http://hdl.handle.net/10379/13720 https://doi.org/10.13025/28655 doi:10.1111/j.1365-2761.2009.01080.x Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ epidemiology infectious pancreatic necrosis virus ireland phylogeny vp2 gene genome segment-a atlantic salmon aquatic birnaviruses salar l scotland fish ipnv identification virulence trout Article 2009 ftnuigalway https://doi.org/10.13025/2865510.1111/j.1365-2761.2009.01080.x 2024-09-17T14:44:30Z This study investigated the genotypes and sub-groups of infectious pancreatic necrosis virus (IPNV) present in farmed and wild salmonid fish in Ireland. An 1100-bp portion of the VP2 region of segment A from each of 55 IPNV isolates collected over 2003-2007 was amplified by reverse-transcription-polymerase chain reaction and the product directly sequenced. The nucleotide sequences of each isolate were aligned and compared with each other and with the corresponding sequences of a number of reference isolates. All the 55 sequenced isolates belonged to genogroup 5 (Sp serotype) and could be divided into two subgroups. Irish subgroup 1 consisted of isolates from farmed salmon originating from an Irish salmon broodstock. Irish subgroup 2 consisted of isolates from imported farmed stock and all reported clinical outbreaks of IPN were associated with isolates from subgroup 2. Isolates from wild fish were identical to some isolates from subgroup 2, and therefore are believed to have originated from infected farms. These results highlight the importance of import risk analysis for diseases not listed under current legislation. Article in Journal/Newspaper Atlantic salmon National University of Ireland (NUI), Galway: ARAN |
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National University of Ireland (NUI), Galway: ARAN |
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ftnuigalway |
language |
unknown |
topic |
epidemiology infectious pancreatic necrosis virus ireland phylogeny vp2 gene genome segment-a atlantic salmon aquatic birnaviruses salar l scotland fish ipnv identification virulence trout |
spellingShingle |
epidemiology infectious pancreatic necrosis virus ireland phylogeny vp2 gene genome segment-a atlantic salmon aquatic birnaviruses salar l scotland fish ipnv identification virulence trout Ruane, N M McCarthy, L J Swords, D Henshilwood, K Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland |
topic_facet |
epidemiology infectious pancreatic necrosis virus ireland phylogeny vp2 gene genome segment-a atlantic salmon aquatic birnaviruses salar l scotland fish ipnv identification virulence trout |
description |
This study investigated the genotypes and sub-groups of infectious pancreatic necrosis virus (IPNV) present in farmed and wild salmonid fish in Ireland. An 1100-bp portion of the VP2 region of segment A from each of 55 IPNV isolates collected over 2003-2007 was amplified by reverse-transcription-polymerase chain reaction and the product directly sequenced. The nucleotide sequences of each isolate were aligned and compared with each other and with the corresponding sequences of a number of reference isolates. All the 55 sequenced isolates belonged to genogroup 5 (Sp serotype) and could be divided into two subgroups. Irish subgroup 1 consisted of isolates from farmed salmon originating from an Irish salmon broodstock. Irish subgroup 2 consisted of isolates from imported farmed stock and all reported clinical outbreaks of IPN were associated with isolates from subgroup 2. Isolates from wild fish were identical to some isolates from subgroup 2, and therefore are believed to have originated from infected farms. These results highlight the importance of import risk analysis for diseases not listed under current legislation. |
format |
Article in Journal/Newspaper |
author |
Ruane, N M McCarthy, L J Swords, D Henshilwood, K |
author_facet |
Ruane, N M McCarthy, L J Swords, D Henshilwood, K |
author_sort |
Ruane, N M |
title |
Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland |
title_short |
Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland |
title_full |
Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland |
title_fullStr |
Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland |
title_full_unstemmed |
Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland |
title_sort |
molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland |
publisher |
Wiley-Blackwell |
publishDate |
2009 |
url |
http://hdl.handle.net/10379/13720 https://doi.org/10.13025/28655 https://doi.org/10.1111/j.1365-2761.2009.01080.x |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Journal of Fish Diseases Ruane, N M; McCarthy, L J; Swords, D; Henshilwood, K (2009). Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in ireland. Journal of Fish Diseases 32 (12), 979-987 0140-7775,1365-2761 http://hdl.handle.net/10379/13720 https://doi.org/10.13025/28655 doi:10.1111/j.1365-2761.2009.01080.x |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.13025/2865510.1111/j.1365-2761.2009.01080.x |
_version_ |
1811636668475637760 |