Detection of salmonid alphavirus rna in celtic and irish sea flatfish
Pancreas disease (PD) caused by the salmonid alphavirus (SAV) has been the most significant cause of mortalities in Irish farmed salmon Salmo salar L. over the past decade. SAV is a single-strand positive-sense RNA virus, originally thought to be unique to salmonids, but has recently been detected u...
Main Authors: | , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Inter-Research Science Center
2014
|
Subjects: | |
Online Access: | http://hdl.handle.net/10379/12730 https://doi.org/10.13025/26354 https://doi.org/10.3354/dao02719 |
id |
ftnuigalway:oai:https://researchrepository.universityofgalway.ie:10379/12730 |
---|---|
record_format |
openpolar |
spelling |
ftnuigalway:oai:https://researchrepository.universityofgalway.ie:10379/12730 2024-09-30T14:32:25+00:00 Detection of salmonid alphavirus rna in celtic and irish sea flatfish McCleary, S Giltrap, M Henshilwood, K Ruane, NM 2014-04-23 http://hdl.handle.net/10379/12730 https://doi.org/10.13025/26354 https://doi.org/10.3354/dao02719 unknown Inter-Research Science Center Diseases of Aquatic Organisms McCleary, S; Giltrap, M; Henshilwood, K; Ruane, NM (2014). Detection of salmonid alphavirus rna in celtic and irish sea flatfish. Diseases of Aquatic Organisms 109 (1), 1-7 0177-5103,1616-1580 http://hdl.handle.net/10379/12730 https://doi.org/10.13025/26354 doi:10.3354/dao02719 Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ sav pancreas disease wild fish horizontal transmission atlantic salmon aquaculture farmed atlantic salmon pancreas disease virus wild marine fish salar l molecular epidemiology nucleotide-sequences north-atlantic ireland waters Article 2014 ftnuigalway https://doi.org/10.13025/2635410.3354/dao02719 2024-09-17T14:44:29Z Pancreas disease (PD) caused by the salmonid alphavirus (SAV) has been the most significant cause of mortalities in Irish farmed salmon Salmo salar L. over the past decade. SAV is a single-strand positive-sense RNA virus, originally thought to be unique to salmonids, but has recently been detected using real-time RT-PCR in a number of wild non-salmonid fish. In the present report, 610 wild flatfish (common dab Limanda limanda, plaice Pleuronectes platessa and megrim Lepidorhombus whiffiagonis) were caught from the Irish and Celtic Seas and screened for SAV using real-time RT-PCR and sequencing. In general, a very low prevalence was recorded in common dab and plaice, except for 1 haul in Dublin Bay where 25% of common dab were SAV-positive. SAV sequence analysis supported the fact that real-time RT-PCR detections were specific and further characterised the detected viruses within SAV Subtype I, the predominant subtype found in farmed salmon in Ireland. Article in Journal/Newspaper Atlantic salmon North Atlantic Salmo salar National University of Ireland (NUI), Galway: ARAN Sav’ ENVELOPE(156.400,156.400,68.817,68.817) |
institution |
Open Polar |
collection |
National University of Ireland (NUI), Galway: ARAN |
op_collection_id |
ftnuigalway |
language |
unknown |
topic |
sav pancreas disease wild fish horizontal transmission atlantic salmon aquaculture farmed atlantic salmon pancreas disease virus wild marine fish salar l molecular epidemiology nucleotide-sequences north-atlantic ireland waters |
spellingShingle |
sav pancreas disease wild fish horizontal transmission atlantic salmon aquaculture farmed atlantic salmon pancreas disease virus wild marine fish salar l molecular epidemiology nucleotide-sequences north-atlantic ireland waters McCleary, S Giltrap, M Henshilwood, K Ruane, NM Detection of salmonid alphavirus rna in celtic and irish sea flatfish |
topic_facet |
sav pancreas disease wild fish horizontal transmission atlantic salmon aquaculture farmed atlantic salmon pancreas disease virus wild marine fish salar l molecular epidemiology nucleotide-sequences north-atlantic ireland waters |
description |
Pancreas disease (PD) caused by the salmonid alphavirus (SAV) has been the most significant cause of mortalities in Irish farmed salmon Salmo salar L. over the past decade. SAV is a single-strand positive-sense RNA virus, originally thought to be unique to salmonids, but has recently been detected using real-time RT-PCR in a number of wild non-salmonid fish. In the present report, 610 wild flatfish (common dab Limanda limanda, plaice Pleuronectes platessa and megrim Lepidorhombus whiffiagonis) were caught from the Irish and Celtic Seas and screened for SAV using real-time RT-PCR and sequencing. In general, a very low prevalence was recorded in common dab and plaice, except for 1 haul in Dublin Bay where 25% of common dab were SAV-positive. SAV sequence analysis supported the fact that real-time RT-PCR detections were specific and further characterised the detected viruses within SAV Subtype I, the predominant subtype found in farmed salmon in Ireland. |
format |
Article in Journal/Newspaper |
author |
McCleary, S Giltrap, M Henshilwood, K Ruane, NM |
author_facet |
McCleary, S Giltrap, M Henshilwood, K Ruane, NM |
author_sort |
McCleary, S |
title |
Detection of salmonid alphavirus rna in celtic and irish sea flatfish |
title_short |
Detection of salmonid alphavirus rna in celtic and irish sea flatfish |
title_full |
Detection of salmonid alphavirus rna in celtic and irish sea flatfish |
title_fullStr |
Detection of salmonid alphavirus rna in celtic and irish sea flatfish |
title_full_unstemmed |
Detection of salmonid alphavirus rna in celtic and irish sea flatfish |
title_sort |
detection of salmonid alphavirus rna in celtic and irish sea flatfish |
publisher |
Inter-Research Science Center |
publishDate |
2014 |
url |
http://hdl.handle.net/10379/12730 https://doi.org/10.13025/26354 https://doi.org/10.3354/dao02719 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon North Atlantic Salmo salar |
genre_facet |
Atlantic salmon North Atlantic Salmo salar |
op_relation |
Diseases of Aquatic Organisms McCleary, S; Giltrap, M; Henshilwood, K; Ruane, NM (2014). Detection of salmonid alphavirus rna in celtic and irish sea flatfish. Diseases of Aquatic Organisms 109 (1), 1-7 0177-5103,1616-1580 http://hdl.handle.net/10379/12730 https://doi.org/10.13025/26354 doi:10.3354/dao02719 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.13025/2635410.3354/dao02719 |
_version_ |
1811636589999161344 |