Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.)

• Published in: BMC Genomics
• Main Authors: Davey, Grace, Houeix, Benoit, Talbot, Anita, Cairns, Michael T.
• Other Authors: |~|
• Format: Article in Journal/Newspaper
• Language: English
• Published: BioMed Central 2016
• Subjects: Trout oncorhynchus-mykiss; Suppression subtractive hybridization; Unfolded protein response; Gene expression profile; Retinol binding protein; Rainbow trout; Cortisol response; Oxidative stress; Atlantic salmon; Plasma cortisol; Biomedical science
• Online Access: http://hdl.handle.net/10379/5666; https://doi.org/10.1186/1471-2164-11-193

Background: Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream ( Sparus aurata) is the main aquacultured species in the Mediterranean area and there is considerable interest in the genetic improvement of this species. With the aim of increasing the genomic resources in gilthead sea bream and identifying genes and mechanisms underlying the physiology of the stress response, we developed a cDNA microarray for gilthead sea bream that is enriched by suppression substractive hybridization with stress and immunorelevant genes. This microarray is used to analyze the dynamics of gilthead sea bream liver expression profile after confinement exposure.Results: Groups of confined and control juvenile fish were sampled at 6, 24, 72 and 120 h post exposure. GeneSpring analyses identified 202 annotated genes that appeared differentially expressed at least at one sampling time (P This work was funded by EU (SSP98-CT-2004-513692; Combined genetic and functional genomic approaches for stress and disease resistance marker assisted selection in fish and shellfish, AQUAFIRST) and Spanish (Ingenio-2010 Programme; Improvement of Aquaculture Production by the use of biotechnological tools, AQUAGENOMICS) projects. AS-V was recipient of a Spanish PhD fellowship from the Diputación Provincial de Castellón. peer-reviewed